Micro- and nanofluidic systems in devices for biological, medical and environmental research

Abstract

Abstract. The use of micro-and nanofluidic systems in modern analytical instruments allow you to implement a number of unique opportunities and achieve ultra-high measurement sensitivity. The possibility of manipulation of the individual biological objects (cells, bacteria, viruses, proteins, nucleic acids) in a liquid medium caused the development of devices on microchip platform for methods: chromatographic and electrophoretic analyzes; polymerase chain reaction; sequencing of nucleic acids; immunoassay; cytometric studies. Development of micro and nano fabrication technologies, materials science, surface chemistry, analytical chemistry, cell engineering have led to the creation of a unique systems such as "lab-on-achip", "human-on-a-chip" and other. This article discusses common in microfluidics materials and methods of making functional structures. Examples of integration of nanoscale structures in microfluidic devices for the implementation of new features and improve the technical characteristics of devices and systems are shown.

Creation of a device for detecting fluorescence from microfluidic chips for studying biosystems

“…In recent years, polymers have taken the leading position as substrate materials for microfluidic devices [42][43][44][45][46]. Them have excellent physical and chemical properties, enabling the creation of micro-sized structures with desired characteristics, which provide microscopic design features that cannot be realized in any other class of materials [49][50][51][52][53][54][55][56].…”