Michaelis-Menten kinetics for enzymatic depolymerization of a linear polyester synthesized from Z-protected glutamic acid

Abstract

A biodegradable polyester resin was polymerized from N‐benzyloxycarbonyl‐L‐glutamic acid and ethylene glycol. Rhizopus delemar lipase was used as a biocatalyst for the rupture of ester bonds during the hydrolysis studies. Depolymerization was observed to follow a Michaelis–Menten mechanism, with the maximum rate of monomer formation dP/dtmax = 1.12 × 10−8 mol/s and the rate constant Km × 2.03 × 10−4 mol. Subject to initial conditions described by the most probable distribution and Michaelis–Menten–type depolymerization rate expressions, population density distribution dynamics of the polymeric molecules that formed the resin were explicitly described using a deterministic approach. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 80: 514–520, 2001

Susceptibility of a Polycaprolactone-based Root Canal–filling Material to Degradation. III. Turbidimetric Evaluation of Enzymatic Hydrolysis

“…The initial velocities of OD reduction V 0 (ie, analogous to that in product-time curves) were deducted from the slopes of the initially linear portion of the OD reduction-time curves (25). Because the reduction in OD was measured rather than the molar concentration of a specific hydrolytic product, V 0 was expressed as "%sec -1 ".…”

Monitoring Biopolymer Degradation by Taylor Dispersion Analysis

“…In the past decade, biodegradable polymers gained interest, especially in the biomedical field, as exemplified by polylactic acid for biomaterial applications, or synthetic polypeptides for biomedical applications, including in vivo drug delivery. − The understanding of the degradation of biopolymers is essential for developing these kinds of applications. Generally, the degradation is monitored by chromatographic methods (e.g., size exclusion chromatography, − HPLC), by electrophoretic methods, , by fluorescence methods or by dynamic light scattering (DLS) …”