Michael-Type Addition of Gelatin on Electrospun Nanofibrils for Self-Assembly of Cell Sheets Composed of Human Dermal Fibroblasts

Lee, Ju Won; Yoo, Hyuk Sang

doi:10.1021/acsomega.9b02602

Cited by 10 publications

(14 citation statements)

References 46 publications

(86 reference statements)

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“…20 Seeded cells were self-assembled into cell sheets with the fibrils in several ways according to the ratio between the cells and the matrix. 21 These cell sheets could be directly transferred to many defect sites, including deepithelized wound sites, osteochondral defect sites, and myogenic defect sites. 22,23 In the present study, we electrospun fibrous meshes with a coaxial nozzle to coeject the gelatin core and the PCL shell, and the gelatin core was cross-linked for further fragmentation into nanofibrils.…”

Section: Introductionmentioning

confidence: 99%

“…When surface-functionalized PCL nanofibers were fragmented in an optimized condition, the fragmented fibrils exhibited features that were significantly different from that of nanofibrous meshes in terms of cell attachment, migration, and proliferation . Seeded cells were self-assembled into cell sheets with the fibrils in several ways according to the ratio between the cells and the matrix . These cell sheets could be directly transferred to many defect sites, including de-epithelized wound sites, osteochondral defect sites, and myogenic defect sites. , …”

Section: Introductionmentioning

confidence: 99%

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Coaxial Electrospun Nanofibers with Different Shell Contents to Control Cell Adhesion and Viability

2020

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Electrospun nanofibers are widely employed as cell culture matrices because their biomimetic structures resemble a natural extracellular matrix. However, due to the limited cell infiltration into nanofibers, three-dimensional (3D) construction of a cell matrix is not easily accomplished. In this study, we developed a method for the partial digestion of a nanofiber into fragmented nanofibers composed of gelatin and polycaprolactone (PCL). The PCL shells of the coaxial fragments were subsequently removed with different concentrations of chloroform to control the remaining PCL on the shell. The swelling and exposure of the gelatin core were manipulated by the remaining PCL shells. When cells were cultivated with the fragmented nanofibers, they were spontaneously assembled on the cell sheets. The cell adhesion and proliferation were significantly affected by the amount of PCL shells on the fragmented nanofibers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Coaxial Electrospun Nanofibers with Different Shell Contents to Control Cell Adhesion and Viability

2020

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“…They have modified PCL by hydrolysis and, subsequently, the aminolysis procedure. Afterward, surface modified PCL was modified by GelMA through Michael-type addition [42]. Michael addition reaction is a popular reaction for the preparation of hydrogel due to their controlling reaction time, different types of bonding with biomolecules, and mild reactivity [12].…”

Section: Michael Addition Reactionmentioning

confidence: 99%

Gelatin Based Hydrogels for Tissue Engineering and Drug Delivery Applications

Amirian¹

2021

Materials Research Foundations

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Gelatin is one of the most popular natural polymers which is widely used in food, pharmaceutical, biomedical and cosmetic industries. The gelatin has been prepared from different sources such as porcine skin, cattle bone, and fish, etc. Depending on the type of acidic and alkali extraction processes the type of gelatin A and B were obtained. This chapter provides a comprehensive overview of preparation of gelatin base hydrogel. Furthermore, we evaluate their method of crosslinking through Schift-base, Mischeal-addition-based, light, UV, chemical, and physical crosslinking. Moreover, due to the unique properties of gelatin they have the ability to immobilize cells and can be applied for stem cell and drug delivery in biomedical applications.

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“…5 For example, GelMA can be cross-linked into covalent hydrogels via nucleophilic Michael type addition with multifunctional thiol linkers. 6 Although Michael-type addition reaction proceeds in a step-growth manner, methacrylate-thiol reactions lack spatial tunability in cross-linking kinetics. In this regard, light-mediated thiol-norbornene chemistry is increasingly used to orthogonal cross-linking of cell-laden hydrogels.…”

Section: Introductionmentioning

confidence: 99%

“…The disadvantages of chain-polymerization can be addressed by forming hydrogels with orthogonal “click chemistry” . For example, GelMA can be cross-linked into covalent hydrogels via nucleophilic Michael type addition with multifunctional thiol linkers . Although Michael-type addition reaction proceeds in a step-growth manner, methacrylate-thiol reactions lack spatial tunability in cross-linking kinetics.…”

Section: Introductionmentioning

confidence: 99%

Dual Functionalization of Gelatin for Orthogonal and Dynamic Hydrogel Cross-Linking

Kim

Nguyen

Chang

et al. 2021

ACS Biomater. Sci. Eng.

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Gelatin-based hydrogels are widely used in biomedical fields because of their abundance of bioactive motifs that support cell adhesion and matrix remodeling. Although inherently bioactive, unmodified gelatin exhibits temperature-dependent rheology and solubilizes at body temperature, making it unstable for three-dimensional (3D) cell culture. Therefore, the addition of chemically reactive motifs is required to render gelatin-based hydrogels with highly controllable cross-linking kinetics and tunable mechanical properties that are critical for 3D cell culture. This article provides a series of methods toward establishing orthogonally cross-linked gelatin-based hydrogels for dynamic 3D cell culture. In particular, we prepared dually functionalized gelatin macromers amenable for sequential, orthogonal covalent cross-linking. Central to this material platform is the synthesis of norbornenefunctionalized gelatin (GelNB), which forms covalently cross-linked hydrogels via orthogonal thiol-norbornene click cross-linking. Using GelNB as the starting material, we further detail the methods for synthesizing gelatin macromers susceptible to hydroxyphenylacetic acid (HPA) dimerization (i.e., GelNB-HPA) and hydrazone bonding (i.e., GelNB-CH) for on-demand matrix stiffening. Finally, we outline the protocol for synthesizing a gelatin macromer capable of adjusting hydrogel stress relaxation via boronate ester bonding (i.e., GelNB-BA). The combination of these orthogonal chemistries affords a wide range of gelatin-based hydrogels as biomimetic matrices in tissue engineering and regenerative medicine applications.

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Michael-Type Addition of Gelatin on Electrospun Nanofibrils for Self-Assembly of Cell Sheets Composed of Human Dermal Fibroblasts

Cited by 10 publications

References 46 publications

Coaxial Electrospun Nanofibers with Different Shell Contents to Control Cell Adhesion and Viability

Coaxial Electrospun Nanofibers with Different Shell Contents to Control Cell Adhesion and Viability

Gelatin Based Hydrogels for Tissue Engineering and Drug Delivery Applications

Dual Functionalization of Gelatin for Orthogonal and Dynamic Hydrogel Cross-Linking

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