Michael Heidelberger and the demystification of antibodies

Epps, Heather L. Van

doi:10.1084/jem.2031fta

Cited by 6 publications

(1 citation statement)

References 7 publications

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“…It was then possible to measure antibodies, which verified for the first time that circulating antibodies were modified serum globulins and not substances of unknown composition merely associated with the globulins of serum. Heidelberger, who was also a talented musician and linguist as well as a very close friend of the 1959 Nobel Prize winner Severo Ochoa de Albornoz, worked in his laboratory until his death at the age of 103 [14]. The biochemical antigen-antibody complex formation was described by Professor John Richardson Marrack, MA, MD in 1934 [15].…”

Section: History Of Immunohistochemistrymentioning

confidence: 99%

Immunohistochemistry in Historical Perspective: Knowing the Past to Understand the Present

Hidalgo

2021

Methods in Molecular Biology

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Immunohistochemistry is an extraordinary and extensively used technique whereby antibodies are used to detect antigens in cells within a tissue section. It has numerous applications in medicine, particularly in cancer diagnosis. It was Albert Hewett Coons, Hugh J Creech, Norman Jones, and Ernst Berliner who conceptualized and first implemented the procedure of immunofluorescence in 1941. They used fluorescein isothiocyanate (FITC)-labelled antibodies to localize pneumococcal antigens in infected tissues. Since then, with improvement and development of protein conjugation, enzyme labels have been introduced, such as peroxidase and alkaline phosphatase. The history of immunohistochemistry (IHC) combines physiology, immunology, biochemistry, and the work of various Nobel Prize laureates. From von Behring who was awarded de first Nobel Prize in 1901 for his work on serum therapy to the 1984 Nobel Prize for the discovery of monoclonal antibodies by Milstein, Kohler, and Jerne, IHC is a story of cooperation and collaboration which led to the development of this magnificent technique that is used daily in anatomical pathology laboratories worldwide.

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Section: History Of Immunohistochemistrymentioning

confidence: 99%