Micelle‐enhanced spectrofluorimetric method for determination of lacidipine in tablet form; application to content uniformity testing

Abstract

A highly sensitive, simple and rapid spectrofluorimetric method was developed for the determination of lacidipine (LCP) in tablets. The proposed method is based on the investigation of the fluorescence spectral behavior of LCP in both sodium dodecyl sulphate (SDS) and the tween-80 micellar system. In aqueous solutions of acetate buffer (pH 4.5), the fluorescence intensities of LCP were greatly enhanced (ca. 2.4 and 4.3 folds) in the presence of either SDS or tween-80, respectively. The fluorescence intensity was measured at 444 nm after excitation at 277 nm using either SDS or tween-80 as a surfactant. The fluorescence-concentration plots were rectilinear over the ranges of 50.0-500.0 ng/ml and 5.0-200.0 ng/ml with lower detection limits of 5.11 and 2.06 ng/ml and lower quantification limits of 17 and 6.87 ng/ml using SDS and tween-80, respectively. The method was successfully applied to the analysis of LCP in commercial tablets and the results were in good agreement with those obtained with the comparison method. Furthermore, content uniformity testing of pharmaceutical tablets was also conducted.

Factorial design optimization of micelle enhanced synchronous spectrofluorimetric assay of Omarigliptin: Applied to content uniformity testing and in vitro drug release

“…It is well known that the addition of a surfactant at a concentration above its critical micelle concentration increases its fluorescence quantum yield . This has been used to enhance the fluorescence of many drugs . The spectrophotometric scan of OMG showed λ maximum at 268 nm that was expected to be close to the excitation wavelength while the spectrofluorimetric scan showed overlapped excitation and emission spectra at 263 and 292 nm, respectively (Figure ).…”

“…The aim of our work is to present a new affordable design of experiment (DOE) optimized micelle enhanced spectrofluorimetric method for determination of OMG. The use of surfactants as fluorescence enhancers was reported with spectrofluorimetry for the pharmaceutical analysis of many drugs because of its inherent sensitivity, low cost and easy operation . Moreover, factorial design has been used by analysts for the analytical method development predicting the best parameters by investigating the combined effect of different factors simultaneously.…”

Micelle‐enhanced direct spectrofluorimetric method for the determination of linifanib: Application to stability studies

“…The quenching effect of methanol, acetone and acetonitrile on the RFI of the micellar system could be attributed to their denaturation effect on the micelles and the micellization process by changing the solvent properties. Also, the influence of short‐chain alcohols on the breakdown of surfactant aggregates has been previously reported …”

Micellar Enhanced Synchronous Fluorescence for the Assay of Sumatriptan Succinate in Pharmaceutical Tablets

“…It is well known that the presence of surfactants may increase the rigidity of the molecules being surrounded by the surfactant which restricts the movement of the fluorophore and so decrease the energy transfer to the surrounding environment, As a result, increases the quantum yield and enhance the fluorescence intensity of the guest molecules [21,23]. The anionic surfactant sodium lauryl sulfate (SLS) has recently found many applications in emission fluorescence enhancement and applications for the determination of some pharmaceutical compounds [24].…”

“…The developed method is considered as a low cost, a time-saving and sensitive method that has the advantages of not using derivatization reactions using toxic chemicals. Moreover, another method of the conventional spectrofluorimetric technique was applied using the synchronous scanning fluorescence at (∆λ = 120 nm), which allowed a simpler spectrum with less overlapping [24].…”