Micellar High-Performance Liquid Chromatographic Separation of Serum Bilirubin Species with Direct Sample Injection

Mei, Lin; Wu, Nian; Aiken, Joseph H.

doi:10.1080/10826079508009286

Cited by 9 publications

(3 citation statements)

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“…Total UCB, sodium, potassium, urea and creatinine were determined by routine spectrophotometry on a P800 unit of a modular analytics serum work area from Roche Diagnostics (Basel, Switzerland). UCB concentrations were confirmed by reverse-phase high-performance liquid chromatography (HPLC) after chloroform extraction as described previously 17 25 26…”

Section: Methodsmentioning

confidence: 99%

“…Bile samples were protected from light, stored at −80 °C under argon directly after collection and processed within 24 h. UCB concentrations were determined by reverse-phased HPLC after chloroform extraction 17 25 26. The biliary bile salt concentration was determined with the 3α-hydroxysteroid dehydrogenase method,27 and bile acid composition was measured by capillary gas chromatography after conversion of bile acids to methyl ester-trimethylsilyl derivatives 10.…”

Section: Methodsmentioning

confidence: 99%

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Acceleration of the gastrointestinal transit by polyethylene glycol effectively treats unconjugated hyperbilirubinaemia in Gunn rats

Cuperus

Iemhoff

Wulp

et al. 2009

Gut

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Gastrointestinal transit time and plasma UCB concentrations are linearly related in Gunn rats. This relationship can be exploited by pharmacologically accelerating the gastrointestinal transit, which increases transmucosal UCB diffusion and thereby effectively treats unconjugated hyperbilirubinaemia. Present results support the feasibility of PEG treatment, either solitary or combined with phototherapy, in patients with severe unconjugated hyperbilirubinaemia.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Acceleration of the gastrointestinal transit by polyethylene glycol effectively treats unconjugated hyperbilirubinaemia in Gunn rats

Cuperus

Iemhoff

Wulp

et al. 2009

Gut

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“…Selectivity in MLC not only depends on the eluent composition but also the solute type and its location on/in the micelles [127]. MLC has been used for the quantitative structure-activity relationship study [128,129], in direct body fluid injection for therapeutic drug monitoring [130][131][132][133][134], for the analysis of nucleotides and bases [135] and for the quantitative determination of bilirubin species [136], in protein separations [137], in the determination of inorganic cations and anions [138,139], and for isomer separations [140].…”

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confidence: 99%

Method development for high performance liquid chromatography: novel organic modifiers and column packing conditions

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This manuscript has been reproduced from the microfilm master. UMl films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer.The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction.In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beguming at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6" x 9" black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. 1996Signature was redacted for privacy.Signature was redacted for privacy.Signature was redacted for privacy. Dissertation OrganizationThis dissertation begins with a general introduction containing a literature review.References cited in the literature review are compiled in the reference section within the general introduction. This is then followed by four chapters all of which are in the format of research papers. They have been accepted, have been submitted or will soon be submitted for publication. A general conclusion section follows the four chapters. The second paper is similar to the publication version, although additional figures and tables have been added.Figures and tables are contained in the text of the paper at the appropriate location.References cited within each paper are listed after the conclusions of each paper. [70]. Indeed, it is amazing just to think about the popularity of HPLC from major components to trace analysis, from food, soil and water to poison, drug and trace evidence, from organic to inorganic analysis, firom natural to synthetic products, from peptides and proteins to RNA and DNA, from industry to clinics, from separation and pxirification to structure elucidation. The future of HPLC seems to be even brighter with gas chromatography, supercritical fluid chromatography, field flow fractionation and capillary electrophoresis to complement it. Higfai Performance Liquid Chromatography 4Although HPLC has become a well established technique, advances are still being made in the fundamental understanding of various separation mechanisms as well as improvements in equipment capabilities.In this dissertation, HPLC methods were developed for the separations of various org...

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Effective oral treatment of unconjugated hyperbilirubinemia in Gunn rats

et al. 2005

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We sought to develop an oral treatment for unconjugated hyperbilirubinemia. In the Gunn rat model of unconjugated hyperbilirubinemia, dietary supplementation with the lipase inhibitor orlistat (Orl) or with calcium phosphate (CaP) decreases plasma unconjugated bilirubin (UCB) levels. We determined whether Orl, CaP, or their combination is superior to phototherapy, the conventional treatment, and whether the effects of Orl and CaP are influenced by dietary fat content. Gunn rats were treated with Orl (200 mg/kg chow), CaP (20 g/kg chow), Orl ؉ CaP, or continuous phototherapy (19 W/cm 2 /nm) during a low-fat (LF) diet (13 energy%) or high-fat (HF) diet (35 energy%). Plasma UCB and fecal fat excretion were measured before, during, and/or at the end of treatment. Orl treatment for 2 weeks (HF diet) reduced plasma UCB concentrations similar to phototherapy (؊34% and ؊28%, respectively); the combination of both was more effective than either treatment alone (؊48%; P < .001). After 3 weeks of a HF diet, plasma UCB was 46% lower compared with the LF diet (P < .001). Plasma UCB concentrations were negatively correlated with fecal fat excretion (r ‫؍‬ ؊0.96; P < .001). Irrespective of dietary fat content, 3 weeks of combined treatment (Orl ؉ CaP) decreased plasma UCB by approximately 50% (P < .01) and was more effective than phototherapy (P < .05) at the intensity provided. C rigler-Najjar disease is characterized by a permanent unconjugated hyperbilirubinemia due to absent (type I) or decreased (type II) activity of the hepatic enzyme bilirubin-UDP-glucuronosyltransferase. 1 Severe unconjugated hyperbilirubinemia can lead to bilirubin encephalopathy, kernicterus, and death. 2,3 Phenobarbital treatment can usually control unconjugated hyperbilirubinemia in Crigler-Najjar type II patients via residual enzyme induction. 4,5 Phenobarbital is not effective in Crigler-Najjar disease type I, however, so these patients have to undergo daily phototherapy, which has considerable disadvantages. Phototherapy becomes less effective with age, probably due to skin alterations, 6,7 a decrease in the surface area to body mass ratio, 8 and a diminishing compliance to the intensive phototherapy regimen, which may take up to 12 hours per day. 6 To prevent irreversible brain damage due to kernicterus, many patients with CriglerNajjar disease type I undergo liver transplantation in their second decade. 9,10 We sought to develop an alternative treatment for unconjugated hyperbilirubinemia based on oral administration and with equal or higher efficacy than phototherapy. The oral treatment strategy used in the present study is based on reducing the reabsorption of UCB 11,12 through intestinal capture. Reabsorption of UCB can contribute substantially to the pathogenesis

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Micellar High-Performance Liquid Chromatographic Separation of Serum Bilirubin Species with Direct Sample Injection

Cited by 9 publications

References 9 publications

Acceleration of the gastrointestinal transit by polyethylene glycol effectively treats unconjugated hyperbilirubinaemia in Gunn rats

Acceleration of the gastrointestinal transit by polyethylene glycol effectively treats unconjugated hyperbilirubinaemia in Gunn rats

Method development for high performance liquid chromatography: novel organic modifiers and column packing conditions

Effective oral treatment of unconjugated hyperbilirubinemia in Gunn rats

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