Micellar electrokinetic chromatography employing sodium alkyl sulfates and Brij 35®

Rasmussen, Henrik T.; Goebel, Lisa K.; McNair, Harold M.

doi:10.1016/s0021-9673(01)95746-2

Cited by 64 publications

(19 citation statements)

References 27 publications

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“…We assume that bulk viscosity will be more influenced by Joule heating than wall surface viscosity. As a result, a small increase of the elution window is observed at higher surfactant concentrations as also reported by others [4,25]. Terabe et al [20], however, reported a small decrease of the elution window, Table 5 Eiectroosmotic mobility, mEo F (10 -5 cm2/Vs), effective mobility of the micelles,mmc (10 5 cm2/Vs), values for the elution window, tMC/tEOV, and measured electric current, I (/xA), at different SDS concentrations, CSD s (mM which may be due to differences in thermoregulating the capillary.…”

Section: Surfactant Concentrationsupporting

confidence: 83%

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Muijselaar

Claessens

Cramers

1995

Journal of Chromatography A

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Section: Surfactant Concentrationsupporting

confidence: 83%

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Muijselaar

Claessens

Cramers

1995

Journal of Chromatography A

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“…Thus they provide greater capacity factors and smaller retention windows [13]. The experimental results obtained with Brij 35, a fatty alcohol ethoxylate with 23 ethyleneoxy groups, showed no separation of the four nitroaromatic compounds.…”

Section: -Nt (20) 23-dnt (21) 2-a-46-dnt (22) 4-a-26-dnt (23) mentioning

confidence: 92%

Separation strategies for the determination of residues of explosives in soils using micellar electrokinetic capillary chromatography

Mussenbrock

Kleiböhmer

1995

J Microcolumn Separations

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Abstract. Micellar electrokinetic capillary chromatography (MEKC) separation strategies for the determination of residues of explosives in soils are presented. Compared to former publications, the number of explosives determined was increased up to 24 compounds in which all EPA priority substances are included. Separation characteristics were investigated as a function of different organic modifiers. Using 1 vol% of methanol, separation was realized for all 24 compounds, including all of the isomers of the monoand dinitrotoluenes. Furthermore, the lipophilic and hydrophilic parts of the surfactant were varied, and the best separation characteristics were obtained with sodium dodecyl sulphate (SDS). Even the suitability of mixed micelles for the analysis of nitroaromatics was tested. With the help of mixed micelles consisting of SDS and the amphoteric surfactant SB-12, separation of 22 out of 24 explosives was realized. With significantly changed selectivities, complete separation required less than 12 minutes.

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“…Operating in such a mode allowed some 10 samples per hour to be analysed. A number of compounds e. g. BRIJ-35 [8], long chain alcohols [9], cyclohexanol [10], 1,2-hexanediol [11], alcohols [12] have been reported to enhanced the separation of standard mixtures of non-polar molecules in MEKC by increasing the migration time window.…”

Section: Originalmentioning

confidence: 99%

Further studies on the MEKC and MEEKC of UV-Absorbing compounds in human urine

et al. 2000

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SummaryMicellar electrokinetic capillary chromatography (MEKC), a mode of capillary electrophoresis, can separate cations, anions and uncharged molecules simultaneously and with high resolution. So it is ideal for separating endogenous and exogenous analytes in complex biofluids, such as urine. Our previous system used 13-CD-enhanced SDS MEKC to aid resolution and could resolve a large numberof peaks from human urine in about 12 minutes.In this study we have investigated a number of alternative additives to enhance resolution even further. Various compounds have been claimed, in the literature, to improve MEKC e.g. mixed micelles, organic solvents, and urea. We investigated a number of such compounds as additives in ourstandard urine system butwithout much success. However some modified cyclodextrins were found to increase both resolution and the migration window. In particular sulphated 13-cyclodextrin gave excellent improvements in both resolution and selectivif,/. The optimised conditions (6.2 mM S-13-CD in 25 mM sodium tetraborate, pH 10, 75 mM SDS) resolved 70 components in a normal urine pool in < 15 minutes using < 10 nl of urine. Faster separations butwith some loss of resolution were obtained by injection from the detector end of the capillary (short-end injection).In addition we have investigated the use of microemulsion electrokinetic chromatography MEEKC but found that it offered no real advantages over MEKC for urine analysis.This optimised MEKC separation is simpler, faster and gives higher resolution than gradient RP-LC.

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Micellar electrokinetic chromatography employing sodium alkyl sulfates and Brij 35®

Cited by 64 publications

References 27 publications

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Separation strategies for the determination of residues of explosives in soils using micellar electrokinetic capillary chromatography

Further studies on the MEKC and MEEKC of UV-Absorbing compounds in human urine

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