Micellar electrokinetic capillary chromatography of haematoporphyrin, protoporphyrin and their copper and zinc complexes

Kiyohara, Chikara; Saitoh, Koichi; Suzuki, N.

doi:10.1016/0021-9673(93)83353-t

Cited by 32 publications

(10 citation statements)

References 12 publications

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“…Similar problems could be observed in other papers [19,21,22], particularly for free porphyrins and their copper complexes, but they were not discussed or eliminated. The possible explanation of the phenomenon is the tendency of some metalloporphyrins to form aggregates.…”

Section: Effect Of Using Cdsmentioning

confidence: 53%

“…5B) of application of CD was explored by Andrighetto et al [22] but only with copper and zinc complexes. Compared to other papers with more metalloporphyrins [19,21,23], the proposed procedure shows better efficiency and a much shorter time of analysis.…”

Section: Effect Of Using Cdsmentioning

confidence: 92%

“…This hypothesis is supported with results obtained by Pasternack et al [31], where the increase of dimerization in the sequence ZnTCPP a TCPP = CuTCPP was observed. The negative effects can be removed by addition of CDs, which form inclusion complexes with porphyrins [21,24,26,32]. Porphyrin molecule is located in the inclusion gap of CD and it reduces the possibility of interaction with other porphyrins.…”

Section: Effect Of Using Cdsmentioning

confidence: 98%

“…The most probable hypothesis for peak broadening is the tendency of porphyrins to form aggregates or the differences in electrophoretic mobility, which result in peak asymmetry. The aggregation and dimerization of water-soluble porphyrins could be reduced by addition of surfactants [19,24], DMF [21], or CDs [22, 25 -27]. The hydrophobic cavity of the CD molecule possesses the ability to form inclusion complexes with a variety of guests, which exerts remarkable effects on important properties of the complexed substances without formation of chemical bonds.…”

Section: Introductionmentioning

confidence: 99%

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Application of cyclodextrins as modifiers in electrophoretic separation of metalloporphyrins

Pyrzyńska¹,

Kilian²

2006

J. Sep. Science

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Several metallocomplexes of tetrakis-carboxyphenylporphyrin (TCPP) were separated on fused-silica capillary using CZE with UV-VIS detection. Metalloporphyrins of Co(II), Cu(II), Mn(II), Ni(II), and Zn(II) were formed directly in TCPP solution with addition of Cd(II) to increase the formation reaction rate. The composition of BGE, its concentration, and pH were optimized to ensure the stability of complexes and proper resolution. In particular, the problem of signals' shape was investigated and discussed. The presence of beta-CD in borate buffer significantly improved separation efficiency and signal shapes due to formation of inclusion complexes. Under the best separation conditions (50 mM borate running buffer at pH 9 with addition of 2 mM beta-CD, 30 kV applied voltage) a separation of metal complexes with TCPP was accomplished in 16 min.

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Section: Effect Of Using Cdsmentioning

confidence: 53%

Section: Effect Of Using Cdsmentioning

confidence: 92%

Section: Effect Of Using Cdsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Application of cyclodextrins as modifiers in electrophoretic separation of metalloporphyrins

Pyrzyńska¹,

Kilian²

2006

J. Sep. Science

View full text Add to dashboard Cite

show abstract

“…The porphyrins form an important group of macrocycles and some of them have been used as electroactive constituents of the membranes [24 ± 26] employed in preparing ISEs. Hematoporphyrin IX is an important porphyrin and shows complexation with Zn 2 [27] and therefore it was thought desirable to prepare its membranes and study them as Zn 2 -selective sensor. The results of these investigations are incorporated in the present communication.…”

Section: Introductionmentioning

confidence: 99%