Mice vaccinated with a non-virulent, aromatic-dependent mutant of Salmonella choleraesuis die from challenge with its virulent parent but survive challenge with Salmonella typhimurium

Nnalue, Ndubisi A.

doi:10.1099/00222615-31-4-225

Cited by 8 publications

(8 citation statements)

References 35 publications

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“…Generally, homologous protection is better than heterologous protection [8,23] but sometimes heterologous challenge with Typhimurium gives better protection than against homologous Choleraesuis [31]. Typhimurium live attenuated strains are also recommended to protect hens against Enteritidis [17,18], or sheep against Abortusovis [24].…”

Section: Discussionmentioning

confidence: 99%

Cross-protection of Salmonella abortusovis, S. choleraesuis, S. dublin and S. gallinarum in mice induced by S. abortusovis and S. gallinarum: bacteriology and humoral immune response

Bernard¹,

Boivin²,

Menanteau³

et al. 2002

Vet. Res.

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Section: Discussionmentioning

confidence: 99%

Cross-protection of Salmonella abortusovis, S. choleraesuis, S. dublin and S. gallinarum in mice induced by S. abortusovis and S. gallinarum: bacteriology and humoral immune response

Bernard¹,

Boivin²,

Menanteau³

et al. 2002

Vet. Res.

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“…Vaccination is one of the most promising control strategies for the reduction of Salmonella in chickens [10]. Cross-protection can enhance the clearance of pathogens through the acquired immune response [10,11]. Salmonella serovars have conserved antigens and including conserved antigens in vaccines might induce cross-protection against multiple serovars [12].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

et al. 2020

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Two experiments were conducted to evaluate the immune response of broilers vaccinated with Salmonella chitosan-nanoparticle (CNP) vaccine and challenged with Salmonella. The Salmonella CNP vaccine was synthesized with Salmonella enterica outer membrane proteins (OMPs) and flagellin proteins. In Experiment I, birds were orally gavaged with PBS or 500, 1000, or 2000μg of CNP vaccine 1 and 7d-of-age. At 14d-ofage, birds were orally challenged with 1 X 10 5 CFU/bird of live S. Enteritidis (SE). Macrophage-nitrite production 11d-post-challenge was higher (P<0.05) in the 500μg group when compared to the control. At d14 (8h-post-challenge), broilers vaccinated with 1000μg CNP had higher (P<0.05) serum anti-OMPs IgG and IgA and cloacal anti-OMP IgA amounts. At 11d-post-challenge, birds vaccinated with 1000μg CNP vaccine had greater (P<0.05) bile anti-OMP and anti-flagellin IgA amounts. At 11d-post-challenge, birds administered 1000μg CNP vaccine has increased (P<0.05) IL-1β and IL-10 mRNA in cecal tonsils. In Experiment II, birds were orally gavaged with PBS or 1000μg CNP or a live commercial vaccine at 1 and 7d-of-age. At 14d-of-age, birds were orally challenged with 1 X 10 5 CFU/bird of live SE or S. Heidelberg (SH). Birds vaccinated with CNP showed higher (P<0.05) serum anti-OMPs IgG amounts at 8h-post-challenge. At 4d-post-SH challenge, birds vaccinated with CNP had higher (P<0.05) bile anti-flagellin IgA amounts. CNP decreased (P<0.05) anti-OMPs IgG levels in serum at 2d-post-SE challenge and 4d-post-SH or SE challenge. Salmonella Enteritidis loads in cecal content at 2d-post-challenge was decreased (P<0.05) by 65.9% in birds vaccinated with CNP, when compared to the control. Chitosan-nanovaccine had no adverse effects on bird's production performance. In conclusion, 1000μg CNP vaccine can induce a specific immune response against Salmonella and has the potential to mitigate SE cecal colonization in broiler birds.

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“…aro mutants are so attenuated that these mutants are avirulent even for a sensitive model such as gnotobiotic pigs (33). The extreme attenuation was probably a reason why in at least some cases the aro mutants were not immunogenic and did not efficiently protect animals from subsequent infection, especially when highly virulent Salmonella strains were used for the challenge (17,20). Despite this, inactivation of aro genes is one of the most frequently used methods for S. enterica attenuation.…”

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confidence: 99%

aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity

et al. 2008

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In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.

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Mice vaccinated with a non-virulent, aromatic-dependent mutant of Salmonella choleraesuis die from challenge with its virulent parent but survive challenge with Salmonella typhimurium

Cited by 8 publications

References 35 publications

Cross-protection of Salmonella abortusovis, S. choleraesuis, S. dublin and S. gallinarum in mice induced by S. abortusovis and S. gallinarum: bacteriology and humoral immune response

Cross-protection of Salmonella abortusovis, S. choleraesuis, S. dublin and S. gallinarum in mice induced by S. abortusovis and S. gallinarum: bacteriology and humoral immune response

Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity

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