Micacocidin A, B and C, Novel Antimycoplasma Agents from Pseudomonas sp. I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Biological Activities.

Kobayashi, Shinobu; Hidaka, Shigetada; Kawamura, Yoshihiro; Ozaki, Mamoru; Hayase, Yoshio

doi:10.7164/antibiotics.51.323

Cited by 43 publications

(20 citation statements)

References 8 publications

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“…A pentyl chain on the salicylate moiety of Ybt is the only structural difference between micacocidin and Ybt (Fig. A) (Kobayashi et al ., 1998a,b; 2000; Kreutzer et al ., ). Moreover, two primary siderophores of Pseudomonads, Pyoverdine I (PVDI) and Pyochelin (Pch) have been shown to bind a wide range of divalent cations including Zn 2+ .…”

Section: Discussionmentioning

confidence: 99%

The Yersinia pestis siderophore, yersiniabactin, and the ZnuABC system both contribute to zinc acquisition and the development of lethal septicaemic plague in mice

Bobrov

Kirillina

Fetherston

et al. 2014

Molecular Microbiology

127

157

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Summary Bacterial pathogens must overcome host sequestration of zinc (Zn2+), an essential micronutrient, during the infectious disease process. While the mechanisms to acquire chelated Zn2+ by bacteria are largely undefined, many pathogens rely upon the ZnuABC family of ABC transporters. Here we show that in Yersinia pestis, irp2, a gene encoding the synthetase (HMWP2) for the siderophore yersiniabactin (Ybt) is required for growth under Zn2+-deficient conditions in a strain lacking ZnuABC. Moreover, growth stimulation with exogenous, purified apo-Ybt provides evidence that Ybt may serve as a zincophore for Zn2+ acquisition. Studies with the Zn2+-dependent transcriptional reporter znuA∷lacZ indicate that the ability to synthesize Ybt affects the levels of intracellular Zn2+. However, the outer membrane receptor Psn and TonB as well as the inner membrane (IM) ABC transporter YbtPQ, that are required for Fe3+ acquisition by Ybt, are not needed for Ybt-dependent Zn2+ uptake. In contrast, the predicted IM protein YbtX, a member of the Major Facilitator Superfamily, was essential for Ybt-dependent Zn2+ uptake. Finally, we show that the ZnuABC system and the Ybt synthetase HMWP2, presumably by Ybt synthesis, both contribute to the development of a lethal infection in a septicemic plague mouse model.

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Section: Discussionmentioning

confidence: 99%

The Yersinia pestis siderophore, yersiniabactin, and the ZnuABC system both contribute to zinc acquisition and the development of lethal septicaemic plague in mice

Bobrov

Kirillina

Fetherston

et al. 2014

Molecular Microbiology

127

157

View full text Add to dashboard Cite

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“…57–250, complexes zinc as well as iron and copper. 73,74 The structure of micacocidin is identical to that of yersiniabactin with the exception of methylation of the nitrogen atom in the saturated thiazolidine ring and substitution of the phenolic ring at the 3-position with an n -pentyl group. Micacocidin forms a complex with Fe(III), known as micacocidin C, but was not investigated as a siderophore until recently.…”

Section: Siderophores As Zincophoresmentioning

confidence: 97%

Beyond iron: non-classical biological functions of bacterial siderophores

2015

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Bacteria secrete small molecules known as siderophores to acquire iron from their surroundings. For over 60 years, investigations into the bioinorganic chemistry of these molecules, including fundamental coordination chemistry studies, have provided insight into the crucial role that siderophores play in bacterial iron homeostasis. The importance of understanding the fundamental chemistry underlying bacterial life has been highlighted evermore in recent years because of the emergence of antibiotic-resistant bacteria and the need to prevent the global rise of these superbugs. Increasing reports of siderophores functioning in capacities other than iron transport have appeared recently, but reports of “non-classical” siderophore functions have long paralleled those of iron transport. One particular non-classical function of these iron chelators, namely antibiotic activity, was even documented before the role of siderophores in iron transport was established. In this Perspective, we present an exposition of past and current work into non-classical functions of siderophores and highlight the directions in which we anticipate that this research is headed. Examples include the ability of siderophores to function as zincophores, chalkophores, and metallophores for a variety of other metals, sequester heavy metal toxins, transport boron, act as signalling molecules, regulate oxidative stress, and provide antibacterial activity.

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“…2B) is a copper-containing compound produced by Pseudomonas sp. with potent activity against various Mycoplasma species [16]. IKD-8344 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Identification of synergists that potentiate the action of polymyxin B against Burkholderia cenocepacia

Loutet

El-Halfawy

Jassem

et al. 2015

International Journal of Antimicrobial Agents

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Burkholderia cenocepacia and other members of the Burkholderia cepacia complex (Bcc) are highly multidrug-resistant bacteria that cause severe pulmonary infections in patients with cystic fibrosis. A screen of 2686 compounds derived from marine organisms identified molecules that could synergize with polymyxin B to inhibit growth of B. cenocepacia. At 1 µg/ml, five compounds synergized with polymyxin B and inhibited the growth of B. cenocepacia by more than 70% compared to growth in polymyxin B alone. Follow-up testing revealed that one compound from the screen, the aminocoumarin antibiotic novobiocin, synergized with polymyxin B and colistin against tobramycin-resistant clinical isolates of B. cenocepacia and Burkholderia multivorans. In parallel, we show that novobiocin sensitivity is common among Bcc species and these bacteria are even more susceptible to an alternative aminocoumarin, clorobiocin, which also had an additive effect with polymyxin B against B. cenocepacia. These studies support using aminocoumarin antibiotics to treat Bcc infections and show that synergizers can be found to increase the efficacy of antimicrobial peptides and polymyxins against Bcc bacteria.

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Micacocidin A, B and C, Novel Antimycoplasma Agents from Pseudomonas sp. I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Biological Activities.

Cited by 43 publications

References 8 publications

The Yersinia pestis siderophore, yersiniabactin, and the ZnuABC system both contribute to zinc acquisition and the development of lethal septicaemic plague in mice

The Yersinia pestis siderophore, yersiniabactin, and the ZnuABC system both contribute to zinc acquisition and the development of lethal septicaemic plague in mice

Beyond iron: non-classical biological functions of bacterial siderophores

Identification of synergists that potentiate the action of polymyxin B against Burkholderia cenocepacia

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