MHC/peptide tetramer-based studies of T cell function

Xu, Xiao-Ning; Screaton, Gavin

doi:10.1016/s0022-1759(02)00196-5

Cited by 29 publications

(20 citation statements)

References 20 publications

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“…24 Tetramers have been described to activate T cells in vivo and to induce activation induced cell death in vitro. 34,35 In our study, priming of HY CTLs must have taken place before tetramer selection. First, unmanipulated cells stained CD45RO positive and gave rise to functional HY-specific T cells.…”

Section: Discussionmentioning

confidence: 99%

Transmaternal cell flow leads to antigen-experienced cord blood

Dierselhuis

Blokland

Pool

et al. 2012

Blood

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Section: Discussionmentioning

confidence: 99%

Transmaternal cell flow leads to antigen-experienced cord blood

Dierselhuis

Blokland

Pool

et al. 2012

Blood

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“…Other MHC class I tetramers; HLA-A2.1-MART-1 [26][27][28][29][30][31][32][33][34][35] , HLA-A2.1-HIVgag 77-85 and HLA-A2.1-EBV 280-288 were purchased from Immunomics. Each MHC class I tetramer was validated by staining of a CTL line speciWc for HLA-A2.1 in association with the peptide of interest.…”

Section: Mhc Class I Tetramersmentioning

confidence: 99%

“…Development and validation of immunologically based treatment strategies is, however, critically dependent on appropriate monitoring of both the immunological response and the clinical response to deWne the correlates of treatment eYcacy [6,25]. The availability of soluble multimeric MHC-peptide complexes has broadened the options for immune monitoring since speciWc CTLs can be identiWed and isolated directly [1,22,34]. In most studies MHC class I tetramers were only eVective for analysis of T cells that are in suspension [24,26].…”

Section: Introductionmentioning

confidence: 99%

In situ detection of antigen-specific T cells in cryo-sections using MHC class I tetramers after dendritic cell vaccination of melanoma patients

Vries

Bernsen

Geloof

et al. 2007

Cancer Immunol Immunother

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Application of tetrameric MHC class I-peptide complexes has signiWcantly improved the monitoring of antigen-speciWc T cell immune responses in mouse models as well as in clinical studies. Especially MHC class I tetramer analysis of tumor-speciWc T cells in suspension or on thick vibratome sections from viable tissue has been proven extremely useful. Using the well-characterized mouse tyrosinase-related-protein-2 speciWc cytotoxic T cell (CTL) clone LP9, we now developed a method that allows for speciWc identiWcation of T cells with MHC class I tetramers in 8 m thick, chemically Wxed cryosections. The protocol was validated in a murine inXuenza virus-infection model. Moreover, analysis of delayed type hypersensitivity (DTH) skin biopsies from melanoma patients vaccinated with peptide-loaded mature dendritic cells, revealed the presence and location of anti-tumor CTLs. The speciWcity of the CTLs detected in situ correlated with both the DTH challenge speciWcity and reactivity of cell suspensions derived from the same biopsies. Collectively, our data demonstrate that in situ MHC class I tetramer staining provides a valuable tool to reveal the presence and anatomical location of speciWc CTLs in frozen tissue following immunebased treatment strategies in cancer patients.

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“…However, this is not a limiting factor in the ex vivo detection system described here. Another structure-based ex vivo detection system is flow cytometric analysis using specific MHC tetramers [25][26][27]. However, thus far there is no suitable MHC class II tetramer that can be used to sensitively detect MBP-reactive T cells.…”

Section: Discussionmentioning

confidence: 99%

Ex vivo detection of myelin basic protein‐reactive T cells in multiple sclerosis and controls using specific TCR oligonucleotide probes

Hong

Zang

et al. 2004

Eur J Immunol

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T cell reactivity to candidate myelin autoantigens, such as myelin basic protein (MBP), may play an important role in the pathogenesis of multiple sclerosis (MS). Although MBP-reactive T cells have been found to undergo in vivo activation in patients with MS, their true precursor frequency in MS is unknown as current frequency analysis is commonly based on the T cell functional responses to MBP. In this study, we developed a TCR sequence-based ex vivo detection system using colony hybridization with oligonucleotide probes specific for CDR3 of selected T cell clones for the analysis of true T cell precursor frequency in PBMC. The results revealed that the precursor frequency of five independent T cell clones recognizing the immunodominant MBP 83-99 region was found to be in the range of 1.6×10 -4 in total T cells in three HLA-DR2 patients with MS compared to that of 0.25×10 -4 in HLA-DR2 healthy individuals. The observed frequency of MBP 83-99 -reactive T cells in MS patients was considerably higher than those measured in parallel by cell culture-based analysis (2.3×10 ) in the same peripheral blood mononuclear cell specimens. Furthermore, the study showed that MBP 83-99 -reactive T cells detected ex vivo belonged to CD45RA + , CD25 + and CD95 -T cell subsets as evidenced by preferential expression of specific TCR transcripts in these cell fractions.

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MHC/peptide tetramer-based studies of T cell function

Cited by 29 publications

References 20 publications

Transmaternal cell flow leads to antigen-experienced cord blood

Transmaternal cell flow leads to antigen-experienced cord blood

In situ detection of antigen-specific T cells in cryo-sections using MHC class I tetramers after dendritic cell vaccination of melanoma patients

Ex vivo detection of myelin basic protein‐reactive T cells in multiple sclerosis and controls using specific TCR oligonucleotide probes

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