MHC class II alpha, beta and MHC class II-associated invariant chains from Chinese sturgeon (Acipenser sinensis) and their response to immune stimulation

Li, Xiuyu; Du, Hejun; Liu, Liu; You, Xiuling; Wu, Mingjiang; Liao, Zhiyong

doi:10.1016/j.fsi.2017.08.042

Cited by 23 publications

(12 citation statements)

References 47 publications

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“…Compared to that, in the octoploid A. schrenckii spleen, 490 unigenes of this KEGG pathway were identified after Y. ruckeri stimulation (Li et al . 2017b).…”

Section: Resultsmentioning

confidence: 99%

“…Li et al . (2017b) characterized MHC II α‐, β‐, and γ‐chain from molecular features in A. sinensis . Expression analysis upon stimulation with poly I:C and with the inactivated Gram‐negative bacterium Vibrio anguillarum revealed a wide tissue distribution, as MHC II α, β and γ genes were expressed in brain, heart, intestine, head kidney, liver, muscle and spleen, with highest expression levels in spleen followed by intestine and head kidney.…”

Section: Resultsmentioning

confidence: 99%

“…In the tetraploid A. dabryanus head kidney, 163 unigenes of the KEGG complement and coagulation pathway were identified upon bacterial Aeromonas hydrophila infection (Luo et al 2018a). Compared to that, in the octoploid A. schrenckii spleen, 490 unigenes of this KEGG pathway were identified after Y. ruckeri stimulation (Li et al 2017b).…”

Section: Complement Systemmentioning

confidence: 99%

See 2 more Smart Citations

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Höhne

Prokopov

Kuhl

et al. 2021

Reviews in Aquaculture

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Sturgeon immunity is relevant for basic evolutionary and applied research, including caviar-and meat-producing aquaculture, protection of wild sturgeons and their re-introduction through conservation aquaculture. Starting from a comprehensive overview of immune organs, we discuss pathways of innate and adaptive immune systems in a vertebrate phylogenetic and genomic context. The thymus as a key organ of adaptive immunity in sturgeons requires future molecular studies. Likewise, data on immune functions of sturgeon-specific pericardial and meningeal tissues are largely missing. Integrating immunological and endocrine functions, the sturgeon head kidney resembles that of teleosts. Recently identified pattern recognition receptors in sturgeon require research on downstream regulation. We review first acipenseriform data on Toll-like receptors (TLRs), type I transmembrane glycoproteins expressed in membranes and endosomes, initiating inflammation and host defence by molecular pattern-induced activation. Retinoic acid-inducible gene-I-like (RIG-like) receptors of sturgeons present RNA and key sensors of virus infections in most cell types. Sturgeons and teleosts share major components of the adaptive immune system, including B cells, immunoglobulins, major histocompatibility complex and the adaptive cellular response by T cells. The ontogeny of the sturgeon innate and onset of adaptive immune genes in different organs remain understudied. In a genomics perspective, our new data on 100 key immune genes exemplify a multitude of evolutionary trajectories after the sturgeon-specific genome duplication, where some single-copy genes contrast with many duplications, allowing tissue specialization, sub-functionalization or both. Our preliminary conclusion should be tested by future evolutionary bioinformatics, involving all >1000 immunity genes. This knowledge update about the acipenseriform immune system identifies several important research gaps and presents a basis for future applications.

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“…Compared to that, in the octoploid A. schrenckii spleen, 490 unigenes of this KEGG pathway were identified after Y. ruckeri stimulation (Li et al . 2017b).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Complement Systemmentioning

confidence: 99%

See 1 more Smart Citation

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Höhne

Prokopov

Kuhl

et al. 2021

Reviews in Aquaculture

View full text Add to dashboard Cite

show abstract

“…Some studies have shown that tuna showed significant upregulation of the CD8 gene after inoculation with alloantigens ( 56 , 57 ), and in zebrafish, the CD8 gene also showed significant upregulation after challenge ( 58 ). MHC-II molecule is mainly to deliver processed antigen fragments to CD4+ T cells during the initiation phase of the immune response and to modulate humoral immunity ( 59 ). In a previous report, it was shown that vaccination upregulated MHC-II mRNA expression level in the head kidney ( 60 ), and in another experiment the number of MHC-II positive cells was increased at the site of infection, suggesting that these cells enhance the immune response by antigen presentation capacity to induce antibody responses ( 61 ).…”

Section: Discussionmentioning

confidence: 99%

An Oral Microencapsulated Vaccine Loaded by Sodium Alginate Effectively Enhances Protection Against GCRV Infection in Grass Carp (Ctenopharyngodon idella)

Qiao

Huo

et al. 2022

Front. Immunol.

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Grass carp reovirus (GCRV) is highly infectious and lethal to grass carp, causing huge economic losses to the aquaculture industry annually. Currently, vaccination is the most effective method against viral infections. Among the various vaccination methods, the oral vaccination is an ideal way in aquaculture. However, low protective efficiency is the major problem for oral vaccination owing to some reasons, such as antigen degradation and low immunogenicity. In our study, we screened the antigenic epitopes of GCRV-II and prepared an oral microencapsulated vaccine using sodium alginate (SA) as a carrier and flagellin B (FlaB) as an adjuvant, and evaluated its protective effects against GCRV-II infection in grass carp. The full length and three potential antigenic epitope regions of GCRV-II VP56 gene were expressed in Escherichia coli and purified by glutathione affinity column respectively. The optimal antigen (VP56-3) was screened by enzyme-linked immunosorbent assay (ELISA). Adjuvant FlaB was also expressed in E. coli and purified by Ni2+ affinity column. Subsequently, we prepared the oral vaccines using sodium alginate as a carrier. The vaccine (SA-VP56-3/FlaB) forms microsphere (1.24 ± 0.22 μm), examined by transmission electron microscopy, scanning electron microscopy, and dynamic light scattering assay. SA-VP56-3/FlaB vaccine has excellent stability, slow-release, and low toxicity by dynamic light scattering assay, release dynamic assay, in vivo fluorescence imaging system, hemolytic activity and cytotoxicity. Then we vaccinated grass carp orally with SA-VP56-3/FlaB and measured immune-related parameters (serum neutralizing antibody titer, serum enzyme activity (TSOD, LZM, C3), immune-related genes ((IgM, IFN1, MHC-II, CD8 in head kidney and spleen), IgZ in hindgut)). The results showed that SA-VP56-3/FlaB significantly induced strong immune responses, compared to other groups. The highest survival rate achieved in SA-VP56-3/FlaB microencapsulated vaccine (56%) in 2 weeks post GCRV challenge, while 10% for the control group. Meanwhile, the tissue virus load in survival grass carp is lowest in SA-VP56-3/FlaB group. These results indicated that SA-VP56-3/FlaB could be a candidate oral vaccine against GCRV-II infection in aquaculture.

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“…Then, the signal is transmitted to IFN regulatory factor 3 (IRF3) to activate MITA (also known as STING, ERIS or MYPS) and TANK binding kinase 1 (TBK1). Major histocompatibility complex class II (MHCII), MHCII, plays an important role in the adaptive immune response by presenting antigens to CD4 + T lymphocytes [72], and CD4L is a subset of T helper cells and [73] is a key marker molecule, which provides a basis for solving the participation of T cells [74]. Consistent with the immune response, the grass carp taking B. s -CotC-VP56-2 rapidly increased IFN1 and MHC-II on the third day, which was significantly higher than the other groups.…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Bacillus subtilis Subunit Vaccine Significantly Enhances the Immune Protection of Grass Carp against GCRV-II Infection

Gao

Huo

Wang

et al. 2021

Viruses

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Grass carp reovirus (GCRV) is a severe virus that causes great losses to grass carp culture every year, and GCRV-II is the current popular and fatal strain. VP56, fibrin on the outer surface of GCRV-II, mediates cell attachment. In this study, we firstly divided the VP56 gene into four fragments to screen the optimal antigen by enzyme-linked immunosorbent assay and neutralizing antibody methods. The second fragment VP56-2 demonstrates the optimal efficiency and was employed as an antigen in the following experiments. Bacillus subtilis were used as a carrier, and VP56-2 was expressed on the surface of the spores. Then, we performed the oral immunization for grass carp and the challenge with GCRV-II. The survival rate was remarkably raised, and mRNA expressions of IgM were significantly up-regulated in spleen and head kidney tissues in the B. s-CotC-VP56-2 group. Three crucial immune indexes (complement C3, lysozyme and total superoxide dismutase) in the sera were also significantly enhanced. mRNA expressions of four important genes (TNF-α, IL-1β, IFN1 and MHC-II) were significantly strengthened. Tissue lesions were obviously attenuated by histopathological slide examination in trunk kidney and spleen tissues. Tissue viral burdens were significantly reduced post-viral challenge. These results indicated that the oral recombinant B. subtilis VP56-2 subunit vaccine is effective for controlling GCRV infection and provides a feasible strategy for the control of fish virus diseases.

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MHC class II alpha, beta and MHC class II-associated invariant chains from Chinese sturgeon (Acipenser sinensis) and their response to immune stimulation

Cited by 23 publications

References 47 publications

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

An Oral Microencapsulated Vaccine Loaded by Sodium Alginate Effectively Enhances Protection Against GCRV Infection in Grass Carp (Ctenopharyngodon idella)

Oral Administration of Bacillus subtilis Subunit Vaccine Significantly Enhances the Immune Protection of Grass Carp against GCRV-II Infection

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