Mhc-B haplotypes in “Campero-Inta” chicken synthetic line

Iglesias, Gabriela; Canet, Zulma Edith; Cantaro, Horacio Luis; Miquel, M. C.; Melo, Julián E.; Miller, Marcia M.; Berres, Mark E.; Fulton, Janet E.

doi:10.3382/ps/pez431

Cited by 13 publications

(22 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The SNP composition of the remaining 19 novel haplotypes is also provided in Figure 1 . Comparison of these 19 novel Silkie haplotypes with novel haplotypes reported in the Finnish Landrace ( Fulton et al., 2017 ) and Argentinian Campero ( Iglesisa et al., 2019 ) showed no shared haplotypes, and thus, these are unique to the Silkie population evaluated in this study. Alignment of BSNP-SLK-Hap13 with the other haplotypes found within the Silkie population shows identical SNP genotypes with BSNP-O03 for the first 64 SNPs, followed by identical SNP genotypes with BSNP-SLK-Hap01 for the last 27 SNPs.…”

Section: Resultsmentioning

confidence: 50%

“…The remaining 21 haplotypes were found at less than 3% in the population, and in many cases, only one individual was identified with that haplotype. More haplotypes were identified within the Silkie population than have been previously reported in individual populations ( Fulton et al., 2016a , Fulton et al., 2017 , Iglesias et al., 2019 ), with the exception of the considerably diverse MHC region in wild caught jungle fowl ( Nguyen-Phuc et al., 2016 ).…”

Section: Resultsmentioning

confidence: 64%

“…Two of the haplotypes from the heritage broiler populations, BSNP-C05 and BSNP-T04, were also found in the Silkie breed. The Argentina Campero chicken synthetic breed was developed by crossing multiple established breeds and was found to contain 11 BSNP haplotypes, sharing BSNP-A08 and BSNP-M01 (both initially found in RIR and WPR breeds) with the Silkie population ( Iglesias et al., 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…Heterozygous individuals were then compared with these initial haplotypes and used to identify additional alternative haplotypes present in the population within heterozygote individuals. Finally, each haplotype found was compared to common haplotypes reported in previous studies using the same SNP panel ( Fulton et al., 2016a , Fulton et al., 2016b , Fulton et al., 2017 , Iglesias et al., 2019 ) to determine if the haplotypes had been previously reported or were novel.…”

Section: Methodsmentioning

confidence: 99%

“…The SNP panel was initially validated using DNA of known serologically defined MHC haplotypes from multiple research lines, as well as standard breeds, heritage breeds, and elite commercial egg production lines from multiple sources. Subsequently, additional haplotypes have been identified in Finnish Landrace populations ( Fulton et al., 2017 ) and an Argentinian Campero population ( Iglesias et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Assessing MHC-B diversity in Silkie chickens

Tarrant

López

Loper³

et al. 2020

Poultry Science

View full text Add to dashboard Cite

The major histocompatibility complex ( MHC ) is a highly polymorphic region on chromosome 16, which contains numerous immune response genes, and is known to influence disease susceptibility and resistance in chickens. Variability of MHC- B haplotypes in various well-known and commercially utilized breeds has previously been identified. This study aims to understand MHC- B diversity in the Silkie breed using a high-density SNP panel that encompasses the chicken MHC- B region. DNA was obtained from 74 females and 27 males from a commercial Silkie breeder colony that is maintained through minimal genetic selection practices. A previously described panel of 90 SNPs, all located within the MHC- B region, was used to evaluate MHC- B variability in the commercial Silkie breeder colony. MHC- B haplotypes identified from the individual SNP information in the Silkie colony were compared to published haplotypes from the same region. Of the 27 haplotypes identified in the Silkie population, 8 have been previously described. Nineteen haplotypes are unique to the Silkie population and include one novel recombinant and 2 additional possible novel recombinants. Six haplotypes were found at a frequency greater than 5% of the population, of which 4 are novel. Finally, Hardy Weinberg Equilibrium ( HWE ) was calculated for the observed haplotypes, which were found to be in HWE. This study shows considerable MHC- B diversity in the Silkie breed and adds further information on variability of the MHC- B region in the chicken.

show abstract

Section: Resultsmentioning

confidence: 50%

Section: Resultsmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Assessing MHC-B diversity in Silkie chickens

Tarrant

López

Loper³

et al. 2020

Poultry Science

View full text Add to dashboard Cite

show abstract

Development and optimization of a hybridization technique to type the classical class I and class II B genes of the chicken MHC

et al. 2019

View full text Add to dashboard Cite

The classical class I and class II molecules of the major histocompatibility complex (MHC) play crucial roles in immune responses to infectious pathogens and vaccines as well as being important for autoimmunity, allergy, cancer and reproduction. These classical MHC genes are the most polymorphic known, with roughly 10,000 alleles in humans. In chickens, the MHC (also known as the BF-BL region) determines decisive resistance and susceptibility to infectious pathogens, but relatively few MHC alleles and haplotypes have been described in any detail. We describe a typing protocol for classical chicken class I (BF) and class II B (BLB) genes based on a hybridization method called reference strand-mediated conformational analysis (RSCA). We optimize the various steps, validate the analysis using well-characterized chicken MHC haplotypes, apply the system to type some experimental lines and discover a new chicken class I allele. This work establishes a basis for typing the MHC genes of chickens worldwide and provides an opportunity to correlate with microsatellite and with single nucleotide polymorphism (SNP) typing for approaches involving imputation.

show abstract