MGr1-Ag is associated with multidrug-resistant phenotype of gastric cancer cells

Shi, Yongquan; Han, Ying; Wang, Xin; Zhao, Yanli; Ning, Xiaoxuan; Xiao, Bing; Fan, Daiming

doi:10.1007/s101200200027

Cited by 30 publications

(26 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…19 Briefly, cells in log phase were plated into 6-well plates (1 ϫ 10 6 cells/well) and cultured overnight at 37°C. After addition of ADR to a final concentration of 5 g/ml, cells continued to be cultured for 1 hr.…”

Section: Fluorescence Intensity Assay Of Intracellular Adrmentioning

confidence: 99%

Overexpression and significance of prion protein in gastric cancer and multidrug‐resistant gastric carcinoma cell line SGC7901/ADR

Pan

Shi

et al. 2004

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

In our previous work, cellular prion protein (PrPc) was identified as an upregulated gene in adriamycin-resistant gastric carcinoma cell line SGC7901/ADR compared to its parental cell line SGC7901. Here we investigate the expression of PrPc in gastric cancer and whether it was involved in multidrug resistance (MDR) of gastric cancer. We demonstrated that PrPc was ubiquitously expressed in gastric cancer cell lines and tissues. PrPc conferred resistance of both P-glycoprotein (P-gp)-related and P-gp-nonrelated drugs on SGC7901, which was accompanied by decreased accumulation and increased releasing amount of adriamycin in PrPc-overexpressing cell line. Inhibition of PrPc expression by antisense or RNAi technology could partially reverse multidrugresistant phenotype of SGC7901/ADR. PrPc significantly upregulated the expression of the classical MDR-related molecule P-gp but not multidrug resistance associated protein and glutathione S-transferase pi. The PrPc-induced MDR could be partially reversed by P-gp inhibitor verapamil. PrPc could also suppress adriamycin-induced apoptosis and alter the expression of Bcl-2 and Bax, which might be another pathway contributing to PrPcrelated MDR. The further study of the biological functions of PrPc may be helpful for understanding the mechanisms of occurrence and development of clinical gastric carcinoma and PrPc-related MDR and developing possible strategies to treat gastric cancer.

show abstract

Section: Fluorescence Intensity Assay Of Intracellular Adrmentioning

confidence: 99%

Overexpression and significance of prion protein in gastric cancer and multidrug‐resistant gastric carcinoma cell line SGC7901/ADR

Pan

Shi

et al. 2004

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several molecules have been found to confer MDR phenotype of tumor cells, such as P-gp, MRP, LRP, BCRP, GSH/GSH, TopoII, apoptosis associated proteins and antiapoptosis proteins. We found the MDR markers are differently over-expressed and no coexpression exists in gastric cancer, MGr1-Ag was a novel MDR protein [5][6][7] . However, these molecules could not interpret completely how tumor cells develop MDR.…”

Section: Discussionmentioning

confidence: 99%

Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells

Zhang

Zhao²,

Pan³

et al. 2003

WJG

View full text Add to dashboard Cite

AIM:To investigate the expression level of ZNRD1 gene in gastric cancer cells SGC7901 and gastric cancer MDR (multidrug resistant) cells SGC7901/VCR, and to observe the drug sensitizing and proliferation effect of ZNRD1 antisense nucleic acid transduction on SGC7901/VCR cells. METHODS:Amplification of sequences encoding ZNRD1 from SGC7901/VCR cDNA by PCR. The levels of ZNRD1 mRNA expression were demonstrated using semiquantitative reverse transcription polymerase chain reaction (RT-PCR). Eukaryotic expression vector pcDNA3.1-anti ZNRD1 was constructed and transfected into SGC7901/VCR cells by lipofectamine. Immunochemical method was used to detect the expression of protein in SGC7901/VCR cells and transfectants. The cell cycle alteration and the intracellular adriamycin (ADM) accumulation were observed by FACS. Growth curve and drug sensitization of cells for vincristine (VCR) were analyzed with MTT assay. RESULTS:We cloned the open reading frame of full-length ZNRD1. The expression of ZNRD1 showed higher in SGC7901/VCR than in SGC7901 cells. The antisense ZNRD1 drug-resistant clones were selected after gene transfection. Immunochemical results showed that the expression level of ZNRD1 protein was lower in anti ZNRD1-SGC7901/VCR cells than that in non-transfectants. Comparing to SGC7901/ VCR and pcDNA3

show abstract

“…Fluorescence intensity of intracellular ADR was determined by flow cytometry as previously reported. 24 Cells at log phase were trypsinized, resuspended and plated into 6-well plates at a cell density of 1 x 10 6 /well. After overnight culture at 37˚C, ADR was added to the medium at a final concentration of 5 µg/ml.…”

Section: Methodsmentioning

confidence: 99%

CIAPIN1 confers multidrug resistance by upregulating the expression of MDR-1 and MRP-1 in gastric cancer cells

Hao

Li²,

Qiao

et al. 2006

Cancer Biology & Therapy

Self Cite

View full text Add to dashboard Cite

In a previous study, we observed that cytokine-induced apoptosis inhibitor 1 (CIAPIN1), a newly identified apoptosis inhibitor, was upregulated at the mRNA level in a multidrugresistant gastric cancer cell line SGC7901/VCR. The aim of this study was to explore the role of CIAPIN1 in the development of multidrug resistance (MDR) in gastric cancer cells. Upregulation of CIAPIN1 in MDR gastric cancer cells was confirmed by semiquantitative RT-PCR and Western blotting. Using cDNA transfection and RNA interference, we successfully established stable transfectants with upregulation (i.e., SGC7901-pCIAPIN1) or downregulation (i.e., SGC7901-pSiCIAPIN1 and SGC7901/ADR-pSiCIAPIN1) of CIAPIN1 expression, respectively. In vitro drug sensitivity assay demonstrated that overexpression of CIAPIN1 conferred MDR in SGC7901 cells whereas downregulation of CIAPIN1 sensitized SGC7901 and SGC7901/ADR cells to anticancer drugs. CIAPIN1 protected both SGC7901 and SGC7901/ADR cells from ADR-induced apoptosis and reduced intracellular accumulation and retention of adriamycin. Moreover, expression of P-glycoprotein (P-gp or MDR-1, a product of MDR-1 gene) and MDR-related protein-1 (MRP-1) was upregulated by CIAPIN1. In addition, Western blotting revealed that CIAPIN1 decreased the expression of Bcl-2, Bax and p53. Therefore, it is concluded that CIAPIN1 confers MDR in gastric cancer cells, likely by upregulating MDR-1 and MRP-1.

show abstract

MGr1-Ag is associated with multidrug-resistant phenotype of gastric cancer cells

Cited by 30 publications

References 14 publications

Overexpression and significance of prion protein in gastric cancer and multidrug‐resistant gastric carcinoma cell line SGC7901/ADR

Overexpression and significance of prion protein in gastric cancer and multidrug‐resistant gastric carcinoma cell line SGC7901/ADR

Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells

CIAPIN1 confers multidrug resistance by upregulating the expression of MDR-1 and MRP-1 in gastric cancer cells

Contact Info

Product

Resources

About