MgO nanoparticles coated with Polyethylene Glycol as carrier for 2-Methoxyestradiol anticancer drug

Abstract

Novel Magnesium Oxide (MgO) nanoparticles (NPs) modified with the polymer poliethylene glycol (PEG) were synthesized as carrier for the anticancer drug 2-Methoxyestradiol (2ME) to improve its clinical application. The functionalized NPs were characterized by Infrared spectroscopy with Fourier transform to elucidate the vibration modes of this conjugate, indicating the formation of the MgO-PEG-2ME nanocomposite. The studies of absorption and liberation determined that MgO-PEG-2ME NPs incorporated 98.51 % of 2ME while liberation of 2ME was constant during 7 days at pH 2, 5 and 7.35. Finally, the MgO-PEG-2ME NPs decreased the viability of the prostate cancer cell line LNCap suggesting that this nanocomposite is suitable as a drug delivery system for anticancer prostate therapy. 12March 15, 2019 1/9 2-Metoxyestradiol (2ME) has antitumor activity in several types of cancer of the 13 reproductive tract as prostate, cervix, ovary or endometrium. 2ME exerts its anticancer 14 activity via anti-proliferative, apoptotic or antiangiogenic effects on tumor cells [7]. 15 Despite to be considerate as a promising anticancer drug it has an unfavorable kinetic 16 with a low solubility in water; Thus, it is necessary to find new ways to facilitate its 17 administration to the human body. In this context, the nanoparticles (NPs) as drug 18 carriers can play a fundamental role to improvement biological parameters. Actually, it 19 has been proposed that polymeric NPs [8] or TiO 2 NPs coated with polyethylene glycol 20 (PEG) could be useful tools to load 2ME [9]. In the searching for new NPs suitable for 21 medical use, MgO NPs are also an excellent candidate because they are bio-friendly [10]. 22 It has been shown that MgO NPs are not toxic for a variety of human cell lines at 23 concentrations under 300 µg/ml [10]. MgO NPs has been also used as a carrier for the 24 antincancer drug doxorubicin indicating its utility for a controlled system of drugs 25 delivery [11, 12]. In this work, we first performed the physicochemical characterization 26 of MgO NPs coated with PEG and loaded with 2ME (MgO-PEG-2ME). The efficiency 27 of absortion and liberation of 2ME was then analyzed. Finally, the effect of 28 MgO-PEG-2ME NPs on the prostate cell line LnCap was assessed. 29 Materials and Methods 30 Synthesis MgO nanoparticles 31 The MgO NPs were obtained by the sol-gel method route assisted with cetyltrimethyl 32 ammonium bromide C 19 H 4 2BrN (CTAB) as a surfactant to reduce the agglomeration of 33 the NPs [13]. 1:1 molar solution of magnesium acetate, Mg(CH 3 COO) 2 4H 2 O (99,5 %, 34 MERK, USA) and tartaric acid C 4 H 6 O 6 to (99,5 %, MERK, USA) was prepared in 35 ethanol and added dropwise over 10 ml of a 0.001 M of CTAB in water at 60 • C. The 36solution was stirred vigorously for 20 hours to achieve gel formation. Once the gel is 37 formed, it was dried and before calcined at 600 • C for 6 hours to give MgO [14]. 38MgO nanoparticles functionalized with PEG and 2ME loading. 39MgO NPs were covered with poliethylene glycol 600 (PEG600; Sigma...

Precipitation of modified Nano molecular Magnesium oxide from Bittern and Ammonia

“…This suggests that the interaction of PEG or EG may occur through hydrogen bonding of the hydroxyl groups on nano Mg(OH) 2 surface. Our results agreed with Aline et al [9].…”

“…Recently, poly ethylene glycols are cheap and have beneficial effect on the microstructure and surface character of Mg(OH) 2 [8]. The nanostructure materials have unique properties due to their interesting physico-chemical properties and wide range of application in Nanodevices [9][10][11] and [12].…”

Injectable niclosamide nanohybrid as an anti-SARS-CoV-2 strategy

“…The band at 1281 cm −1 corresponds to C-O stretching vibration in PEG molecule, which was less intensified after coating with BSA-NIC NPs. The band at 1242 cm −1 in PEG corresponds to CH 2 twisting, and it has got less intensified in PEG coated BSA-NIC NPs, possibly due to a hydrogen bonding characteristic property [ 50 ].…”

Impact of Magnesium Oxide Nanoparticles on Erythropoietin Hormone Levels in Sera of Patients with Anemia Accompanied with Diabetic Kidney Disease

“…Alfaro et al [37] showed that magnesium was an effective drug dlivery after coating with polymer polyethylene glycol as the carrier for the 2-methoxyestradiol cancer treatment drug. Small particle size of MgO NPs as shown from TEM was an significant determinant of their biocompatibility which could be model for internalization in the intracellular [37].…”

“…Alfaro et al [37] showed that magnesium was an effective drug dlivery after coating with polymer polyethylene glycol as the carrier for the 2-methoxyestradiol cancer treatment drug. Small particle size of MgO NPs as shown from TEM was an significant determinant of their biocompatibility which could be model for internalization in the intracellular [37]. Protein therapeutics such as rhuEPO injected in the skin may see internalization and biological activity restricted due to uneasy absorbance into the skin because of the very low permeability; therefore, conjugated nanoparticles with rhuEPO enhanced skin penetration properties and gave transcutaneous protein delivery efficiency [38].…”