2009
DOI: 10.1016/j.bpj.2008.12.1824
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MG53 Nucleates Assembly Of Cell Membrane Repair Machinery

Abstract: Ca 2þ -triggered membrane fusion, the defining step of exocytosis, enables temporal/spatial control over the release of biologically active compounds. The mechanism by which Ca 2þ triggers and modulates native membrane fusion is still poorly understood. As an unbiased approach to investigating this process, the effects of several thiol-reactive reagents on the homotypic fusion of isolated cortical vesicles (a stage-specific preparation for analyses of native Ca 2þ -triggered fusion) have been characterized. Su… Show more

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Cited by 62 publications
(159 citation statements)
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“…In regards to the Kd values, phosphatidylserine was determined to be the most tightly bound lipid. This result is consistent with data from the previous lipid profiling experiments (2). In order to better understand the binding activation of the phospholipid head group for TRIM72, we conducted an additional binding experiment with ethylenediamine, which is a head analog group of phosphatidylethanolamine.…”
Section: Lipid Binding Of Trim72supporting
confidence: 88%
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“…In regards to the Kd values, phosphatidylserine was determined to be the most tightly bound lipid. This result is consistent with data from the previous lipid profiling experiments (2). In order to better understand the binding activation of the phospholipid head group for TRIM72, we conducted an additional binding experiment with ethylenediamine, which is a head analog group of phosphatidylethanolamine.…”
Section: Lipid Binding Of Trim72supporting
confidence: 88%
“…However, the biochemical features of molecules that bind to TRIM72 have not been explicitly determined through in vitro binding experiments. To date, in vitro analyses have been carried out using only water-soluble constructs such as tagged TRIM72 proteins or deletion mutants (2,8). In the current study, the binding properties of untagged/undeleted and intact TRIM72 were evaluated.…”
Section: Discussionmentioning
confidence: 99%
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