MG160, a Membrane Protein of the Golgi Apparatus Which Is Homologous to a Fibroblast Growth Factor Receptor and to a Ligand for E-selectin, Is Found Only in the Golgi Apparatus and Appears Early in Chicken Embryo Development

Stieber, Anna; Mourelatos, Z; Chen, Y J; Douarin, Nicole Le; Gonatas, N. K.

doi:10.1006/excr.1995.1265

Cited by 30 publications

(19 citation statements)

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“…35,36). ESL-1 is a major E-selectin ligand on murine myelocytes and has high homology to the type 1 sialo-membrane protein of the medial cisternae of the Golgi apparatus, MG-160 (35)(36)(37). To confirm the localization of ESL-1 at 150 kDa, we immunoblotted MDA PCa 2b membrane protein and membrane protein from ESL-1-positive WEHI-3 cells with rabbit immunoglobulin G antisera raised against ESL-1.…”

Section: Resultsmentioning

confidence: 99%

Identification of Leukocyte E-Selectin Ligands, P-Selectin Glycoprotein Ligand-1 and E-Selectin Ligand-1, on Human Metastatic Prostate Tumor Cells

et al. 2005

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Prostate tumor cells, which characteristically metastasize to bone, initiate binding interactions with bone marrow endothelium under blood flow conditions through binding interactions with E-selectin. We hypothesized that E-selectin ligands on prostate tumor cells are directly associated with bone-metastatic potential. In this report, we elucidate the identity of E-selectin ligands on human metastatic prostate tumor cells and examine their association with prostate tumor progression and metastasis in vivo. To our surprise, we found that the E-selectin-binding form of P-selectin glycoprotein ligand-1 (PSGL-1) is expressed on the human bone-metastatic prostate tumor MDA PCa 2b cell line. Interestingly, we also found that human prostate tumor cells derived from bone, lymph node, and brain metastases expressed another leukocyte E-selectin ligand, E-selectin ligand-1 (ESL-1). Immunohistochemical analysis of PSGL-1 and ESL-1 in normal prostate tissue and in localized and metastatic prostate tumors revealed that ESL-1 was principally localized to intracellular cell membrane and expressed on all normal and malignant prostate tissue, whereas PSGL-1 was notably detected on the surfaces of bone-metastatic prostate tumor cells. These findings implicate a functional role of PSGL-1 in the bone tropism of prostate tumor cells and establish a new perspective into the molecular mechanism of human prostate tumor metastasis. (Cancer Res 2005; 65(13): 5750-60)

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Section: Resultsmentioning

confidence: 99%

Identification of Leukocyte E-Selectin Ligands, P-Selectin Glycoprotein Ligand-1 and E-Selectin Ligand-1, on Human Metastatic Prostate Tumor Cells

et al. 2005

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“…MG160 is a marker of the medial Golgi that has homology to basic fibroblast growth factor receptor. It is sialylated but has not been shown to cycle to the plasma membrane (Gonatas et al, 1989;Stieber et al, 1995). In control cells, ( Figure 7A), TGN38 is localized to the three trans-most cisternae.…”

Section: Comparison Of Medial-and Trans-reporter Molecules In Controlmentioning

confidence: 95%

Structure of the Golgi and Distribution of Reporter Molecules at 20°C Reveals the Complexity of the Exit Compartments

Ladinsky

McIntosh

et al. 2002

MBoC

126

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Incubating cells at 20°C blocks transport out of the Golgi complex and amplifies the exit compartments. We have used the 20°C block, followed by EM tomography and serial section reconstruction, to study the structure of Golgi exit sites in NRK cells. The dominant feature of Golgi structure in temperature-blocked cells is the presence of large bulging domains on the three trans-most cisternae. These domains extend laterally from the stack and are continuous with "cisternal" domains that maintain normal thickness and alignment with the other stacked Golgi cisternae. The bulging domains do not resemble the perpendicularly extending tubules associated with the trans-cisternae of control cells. Such tubules are completely absent in temperatureblocked cells. The three cisternae with bulging domains can be identified as trans by their association with specialized ER and the presence of clathrin-coated buds on the trans-most cisterna only. Immunogold labeling and immunoblots show a significant degradation of a medialand a trans-Golgi marker with no evidence for their redistribution within the Golgi or to other organelles. These data suggest that exit from the Golgi occurs directly from three trans-cisternae and that specialized ER plays a significant role in trans-Golgi function.

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“…COMP is normally expressed at high levels in the territorial matrix of chondrocytes and is believed to belong to the family of thrombospondin genes (Newton et al 1994); mutations of COMP have been found in patients with pseudoachondroplasia or multiple epiphyseal dysplasia (Ikegawa et al 1998), indicating its important role in maintaining cartilaginous structure (Thur et al 2001). GLG1 encodes a conserved membrane sialoglycoprotein present in the Golgi apparatus of most cells; this protein binds with basic fibroblast growth factor (FGFB) and is expressed during chondrogenesis (Stieber et al 1995). GPS2 encodes a 327-amino-acid polypeptide with no similarity to other proteins or known motifs; however, this molecule potently regulates RAS-and mitogenactivated protein kinase (MAPK)-mediated signals and interferes with JNK activity in yeast and mammalian cells (Spain et al 1996).…”

Section: Identification Of Separate Marker Genes For Hyaline Cartilagmentioning

confidence: 99%

Expression profiles of two types of human knee-joint cartilage

et al. 2003

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We have performed a comprehensive analysis of gene-expression profiles in human articular cartilage (hyaline cartilage) and meniscus (fibrocartilage) by means of a cDNA microarray consisting of 23,040 human genes. Comparing the profiles of the two types of cartilage with those of 29 other normal human tissues identified 24 genes that were specifically expressed in both cartilaginous tissues; these genes might be involved in maintaining phenotypes common to cartilage. We also compared the cartilage profiles with gene expression in human mesenchymal stem cells (hMSC), and detected 22 genes that were differentially expressed in cells representing the two cartilaginous lineages, 11 specific to each type, which could serve as markers for predicting the direction of chondrocyte differentiation. Our data should also provide useful information about regeneration of cartilage, especially in support of efforts to identify cartilage-specific molecules as potential agents for therapeutic approaches to joint repair.

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MG160, a Membrane Protein of the Golgi Apparatus Which Is Homologous to a Fibroblast Growth Factor Receptor and to a Ligand for E-selectin, Is Found Only in the Golgi Apparatus and Appears Early in Chicken Embryo Development

Cited by 30 publications

References 0 publications

Identification of Leukocyte E-Selectin Ligands, P-Selectin Glycoprotein Ligand-1 and E-Selectin Ligand-1, on Human Metastatic Prostate Tumor Cells

Identification of Leukocyte E-Selectin Ligands, P-Selectin Glycoprotein Ligand-1 and E-Selectin Ligand-1, on Human Metastatic Prostate Tumor Cells

Structure of the Golgi and Distribution of Reporter Molecules at 20°C Reveals the Complexity of the Exit Compartments

Expression profiles of two types of human knee-joint cartilage

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