Mg-dechelation of chlorophyll a by Stay-Green activates chlorophyll b degradation through expressing Non-Yellow Coloring 1 in Arabidopsis thaliana

Sato, Tomoaki; Shimoda, Yousuke; Matsuda, Kaori; Tanaka, Ayumi; Itô, Hisashi

doi:10.1016/j.jplph.2018.01.010

Cited by 54 publications

(30 citation statements)

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“…The chlorina mutant of barley lacks chlorophyll b; the thylakoid membrane polypeptide [51]. When Stay-Green is transiently overexpressed in mutant of arabidopsis , both chlorophyll a and b are degraded, and an NYC1 -deficient mutant did not degrade chlorophyll b and LHCII under SGR overexpression [50, 52]. In this study, LHCs (20-24) were significantly decreased in YY when compared to the wild type plants.…”

Section: Discussionmentioning

confidence: 77%

“…The LHC is an important component of light harvesting complex in the thylakoid membrane, as the protein can capture and transduct the light energy, increasing the light energy capture efficiency and adjusting the light energy distribution [49]. All the chlorophyll-binding proteins decreased in NYC1 like mutant [50]. The chlorina mutant of barley lacks chlorophyll b; the thylakoid membrane polypeptide [51].…”

Section: Discussionmentioning

confidence: 99%

“…Photosynthesis in leaves provides the basis for grain formation in crop production [35]. Leaf color mutants are valuable materials for understanding chloroplast development, chlorophyll biosynthesis and metabolism, photosynthesis, and for identifying the functions of genes [37, 50, 55]. Biosynthesis and expression of genes might be strongly perturbed by mutation [59].…”

Section: Discussionmentioning

confidence: 99%

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Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

Gao

Sang

Chen

et al. 2018

Preprint

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11Leaf color mutation in sesame always affects the growth and development of plantlets, and their 12 yield. To clarify the mechanisms underlying leaf color regulation in sesame, we analyzed a 13 yellow-green leaf mutant. Genetic analysis of the mutant selfing revealed 3 phenotypes-YY, 14 light-yellow (lethal); Yy, yellow-green; and yy, normal green-controlled by an incompletely 15 dominant nuclear gene, Siyl-1. In YY and Yy, the number and morphological structure of the 16 chloroplast changed evidently, with disordered inner matter, and significantly decreased 17 chlorophyll content. To explore the regulation mechanism of leaf color mutation, the proteins 18 expressed among YY, Yy, and yy were analyzed. All 98 differentially expressed proteins (DEPs) 19 were classified into 5 functional groups, in which photosynthesis and energy metabolism 20 (82.7%) occupied a dominant position. Our findings provide the basis for further molecular 21 mechanism and biochemical effect analysis of yellow leaf mutants in plants. 22 24which also contain numerous beneficial minerals, antioxidants, and multi-vitamins [1]. 25Compared with other oilseed crops, sesame is still a low yield crop with low harvest index. 26The key research objectives in sesame are to increase the photosynthesis efficiency and the 27 yield per square area. 28Leaf color is an important trait related with the chlorophyll content, and always affects the 29 photosynthesis efficiency and the final productivity [2]. In plants, the chloroplast structure 30 always alters the composition and content of photosynthetic pigments. Several studies on the 31 leaf color mutants have uncovered a wide range of leaf color mutation types [3]. Most 32 mutagenesis of leaf color were related to the structure and function of the chloroplast [4], 33 chlorophyll biosynthesis and degradation mechanisms [5], photosynthesis [6], chloroplast 34 developmental characteristics [7], genetics [8], and molecular mechanisms [9]. Furthermore, 35some mutants were used for crop breeding to higher biomass, quality, and/or other specific 36 characteristics [10]. There is a rich diversity of leaf color mutation types in plants [11][12][13][14]. 37Leaf color mutants were divided into five types according to the color classification albino, 38 yellowing, light-green, stripe, spot, etc [15, 16]. Falbel [12] divided the chlorophyll mutant 39 into two categories: (1) mutants that lack chlorophyll b, such as arabidopsis mutants [17], and 40(2) mutants with reduced synthesis of total chlorophyll and chlorophyll b; currently most 41 mutants belong to the latter category. Leaf color characteristics controlled by both nuclear 42 inheritance and cytoplasmic inheritance, may be a quantitative or a quality trait [18]. Most of 43 the leaf color variations are caused by nuclear gene mutation inducing a type of chlorophyll 44 deficiency in higher plants. The leaf color variations are mostly monogenic mutation, while a 45 handful of them are polygenic mutation. Among these mutations, the recessive mutations are 4...

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

Gao

Sang

Chen

et al. 2018

Preprint

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show abstract

“…The LHC is an important component of the light harvesting complex in the thylakoid membrane, because the protein can capture and transduce light energy, increasing the light energy capture efficiency and adjusting the light energy distribution (Xu et al 2012). All the chlorophyllbinding proteins decreased in the NYC1-like mutant (Sato et al 2018). The chlorina mutant of barley lacks chlorophyll b and the thylakoid membrane polypeptide (Bellemare et al 1982).…”

Section: Functional Analysis Of Depsmentioning

confidence: 99%

Cytological, genetic, and proteomic analysis of a sesame (Sesamum indicum L.) mutant Siyl-1 with yellow–green leaf color

et al. 2019

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Background Both photosynthetic pigments and chloroplasts in plant leaf cells play an important role in deciding on the photosynthetic capacity and efficiency in plants. Systematical investigating the regulatory mechanism of chloroplast development and chlorophyll (Chl) content variation is necessary for clarifying the photosynthesis mechanism for crops. Objective This study aims to explore the critical regulatory mechanism of leaf color mutation in a yellow-green leaf sesame mutant Siyl-1. Methods We performed the genetic analysis of the yellow-green leaf color mutation using the F 2 population of the mutant Siyl-1. We compared the morphological structure of the chloroplasts, chlorophyll content of the three genotypes of the mutant F 2 progeny. We performed the two-dimensional gel electrophoresis (2-DE) and compared the protein expression variation between the mutant progeny and the wild type. Results Genetic analysis indicated that there were 3 phenotypes of the F 2 population of the mutant Siyl-1, i.e., YY type with light-yellow leaf color (lethal); Yy type with yellow-green leaf color, and yy type with normal green leaf color. The yellowgreen mutation was controlled by an incompletely dominant nuclear gene, Siyl-1. Compared with the wild genotype, the chloroplast number and the morphological structure in YY and Yy mutant lines varied evidently. The chlorophyll content also significantly decreased (P < 0.05). The 2-DE comparison showed that there were 98 differentially expressed proteins (DEPs) among YY, Yy, and yy lines. All the 98 DEPs were classified into 5 functional groups. Of which 82.7% DEPs proteins belonged to the photosynthesis and energy metabolism group. Conclusion The results revealed the genetic character of yellow-green leaf color mutant Siyl-1. 98 DEPs were found in YY and Yy mutant compared with the wild genotype. The regulation pathway related with the yellow leaf trait mutation in sesame was analyzed for the first time. The findings supplied the basic theoretical and gene basis for leaf color and chloroplast development mechanism in sesame. Keywords Sesame • 2-DE • Proteomic • Yellow-green leaf mutant • Photosynthesis Abbreviations ATPase Adenosine triphosphate synthase ATPQ ATP synthase subunit d Chl Chlorophyll Cm Chloroplast outer membrane Ct Chloroplast CYP 38 Cyclophilin 38 Cyt b6-f Cytochrome b6-f complex iron-sulfur subunit 2-DE Two-dimensional gel electrophoresis DEPs Differentially expressed proteins EMS Ethylmethylsulfone FBA Fructose-bisphosphate aldolase FNR Ferredoxin-NADP reductase GAPA Glyceraldehyde-3-phosphate dehydrogenase A GAPDH Glyceraldehyde-3-phosphate dehydrogenase Gr Grana GS2 Glutamine synthetase

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“…However, Fe 3+ , Mg 2+ , Ca 2+ , and Mn 2+ as impurities in WPA are necessary medium trace elements for the growth of plants. It is well‐known that magnesium is an essential element in chlorophyll, iron is an important plant material of aerobic respiration enzymes (it also participates in the formation of chlorophyll), and calcium can promote a variety of metabolic processes in the plant body . On the one hand, the removal of impurities (Fe 3+ , Mg 2+ , Ca 2+ , and Mn 2+ ) is necessary to produce high purity fertilizer; however, on the other hand, these medium trace elements (Fe, Mg, Ca, and Mn elements of chelating state) are added to base high purity fertilizer to produce compound fertilizer .…”

Section: Introductionmentioning

confidence: 99%

Chelation of metal ions with citric acid in the ammoniation process of wet‐process phosphoric acid

Wan

Luo

et al. 2019

Can J Chem Eng

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A new process is proposed in which citric acid is added during the ammoniation process of wet-process phosphoric acid (WPA) to chelate metal ions to produce fertilizer ammonium dihydrogen phosphate (MAP) containing medium trace elements. The parameters of the chelation reaction were studied systematically and the results show that the optimal reaction condition is pH 3.0, reaction time 60 minutes, temperature 50.0 C, and citric acid content 1.48%. The XRD patterns show that the waterinsoluble compounds are complex mixtures, and the composition of the waterinsoluble compounds are changed through the addition of citric acid. FTIR and XPS analyes further demonstrate that water-insoluble compounds are complex mixtures. The XRD patterns of the MAP products indicate that the structure and morphology of the MAP were changed by the addition of citric acid. In addition, the contents of major elements, medium elements, and trace elements in the MAP product are 68.72%, 0.24%, and 0.50%, respectively, which is consistent with the relevant China national standard. K E Y W O R D Sammonium dihydrogen phosphate, chelation, citric acid, wet-process phosphoric acid

show abstract

Mg-dechelation of chlorophyll a by Stay-Green activates chlorophyll b degradation through expressing Non-Yellow Coloring 1 in Arabidopsis thaliana

Cited by 54 publications

References 43 publications

Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

Cytological, genetic, and proteomic analysis of a sesame (Sesamum indicum L.) mutant Siyl-1 with yellow–green leaf color

Chelation of metal ions with citric acid in the ammoniation process of wet‐process phosphoric acid

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