MG-63 osteoblast-like cell proliferation on auxetic PLGA scaffold with mechanical stimulation for bone tissue regeneration

Abstract

BackgroundAuxetic scaffolds (experimental) was fabricated by using poly(D, L-lactic-co-glycolic acid), 50:50, (PLGA) for effective bone cell proliferation with mechanical stimulation.MethodsNegative Poisson’s ratio in scaffold, 3-directional volumetric compression was applied during the scaffold fabrication at adequate temperature (60 °C). The pore size of scaffold ranged between 355 and 400 μm.ResultsThe porous morphology of the prepared auxetic scaffolds had shown partially concave and dent shapes in SEM image as expected. The lowest Poisson’s ratios of experimental group was −0.07 at 60 °C/10 min. Compressive strength of experimental group was shown about 3.12 times higher than control group (conventional scaffold) in dry state at 25 °C. The compressive strengths of both groups were tended to be decreased dramatically in wet state compared to in dry state. However, compressive strengths of experimental group were higher 3.08 times and 1.88 times in EtOH/PBS (25 °C) and EtOH/PBS/DMEM (37 °C) than control group in wet state, respectively. Degradation rate of the scaffolds showed about 16 % weight loss in 5 weeks. In cell attachment test, experimental group showed 1.46 times higher cell proliferation than control group at 1-day with compressive stimulation. In 3-day culture, the experimental group showed 1.32 times higher than control group. However, there was no significant difference in cell proliferation in 5-day cultivation.ConclusionOverall, negative Poisson’s ratio scaffolds with static mechanical stimulation could affect the cell proliferation at initial cultivation time.

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MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold

“…The convex porous PLGA scaffold was successfully transformed into a reentrant porous form that had special pore structures and NPR. Our previous study revealed that this structure could deliver a compressive load isotropically to cells in the scaffold, and it had an effect on cell proliferation [33,34]. In this study we investigated which type of compressive stimulation, that is, static or dynamic, could be more effective in bone cell proliferation.…”