MG‐63 human osteosarcoma cells grown in monolayer and as three‐dimensional tumor spheroids present a different metabolic profile: a <sup>1</sup>H NMR study

Santini, Maria Teresa; Rainaldi, Gabriella; Romano, R.; Ferrante, Antonella; Clemente, Stefania; Motta, Andréa; Indovina, Pietro Luigi

doi:10.1016/s0014-5793(03)01466-2

Cited by 51 publications

(35 citation statements)

References 36 publications

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“…This observation indicates that the HIPE-based macroporous 3D environment encourages the osteosarcoma cell line to behave in a more natural way, forming tumour-like spheroids without the requirement of any external manipulation. Previous studies have also shown similar findings where MG63 spheroids have been developed (i) in ex vivo bone formation models and (ii) in in vitro 3D culture systems, for example, using silicate-based hydrogels [29][30][31] ; but the observation of culturing tumour-spheroids in a 3D in vitro structured scaffold has to our knowledge not yet been reported. Overall, the development of PolyHIPE-based 3D scaffolds offers more realistic opportunities for replicating in vivo behavior of cell environments.…”

Section: Osteosarcoma Growthsupporting

confidence: 71%

Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Malayeri¹,

Sherborne²,

Paterson³

et al. 2024

IJB

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This paper describes the direct laser write of a photocurable acrylate-based PolyHIPE (High Internal Phase Emulsion) to produce scaffolds with both macro-and microporosity, and the use of these scaffolds in osteosarcoma-based 3D cell culture. The macroporosity was introduced via the application of stereolithography to produce a classical "woodpile" structure with struts having an approximate diameter of 200 μm and pores were typically around 500 μm in diameter. The PolyHIPE retained its microporosity after stereolithographic manufacture, with a range of pore sizes typically between 10 and 60 μm (with most pores between 20 and 30 μm). The resulting scaffolds were suitable substrates for further modification using acrylic acid plasma polymerisation. This scaffold was used as a structural mimic of the trabecular bone and in vitro determination of biocompatibility using cultured bone cells (MG63) demonstrated that cells were able to colonise all materials tested, with evidence that acrylic acid plasma polymerisation improved biocompatibility in the long term. The osteosarcoma cell culture on the 3D printed scaffold exhibits different growth behaviour than observed on tissue culture plastic or a flat disk of the porous material; tumour spheroids are observed on parts of the scaffolds. The growth of these spheroids indicates that the osteosarcoma behave more akin to in vivo in this 3D mimic of trabecular bone. It was concluded that PolyHIPEs represent versatile biomaterial systems with considerable potential for the manufacture of complex devices or scaffolds for regenerative medicine. In particular, the possibility to readily mimic the hierarchical structure of native tissue enables opportunities to build in vitro models closely resembling tumour tissue.

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Section: Osteosarcoma Growthsupporting

confidence: 71%

Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Malayeri¹,

Sherborne²,

Paterson³

et al. 2024

IJB

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“…In this way, differences in the results were independent from the cell density but due to the different conditions tested. In fact, considering a diameter of 25 to 30 mm for MG-63 cells, 55 the volume fraction of cells 56,57 in monolayer is 0.52 to 0.75 that gives an effective electric field that is 84% to 95% of the applied one. From these data it was determined that by applying 1000 V/ cm the transmembrane voltage is 3.15 to 8.56 V. This transmembrane voltage is larger with respect to the typical one to form cell membrane pores 58 (ie, 0.2-1 V).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Electroporation Efficiency of a Grid Electrode for Electrochemotherapy

Ongaro

Campana

Mattei

et al. 2015

Technol Cancer Res Treat

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Electrochemotherapy (ECT) is a local anticancer treatment based on the combination of chemotherapy and short, tumorpermeabilizing, voltage pulses delivered using needle electrodes or plate electrodes. The application of ECT to large skin surface tumors is time consuming due to technical limitations of currently available voltage applicators. The availability of large pulse applicators with few and more spaced needle electrodes could be useful in the clinic, since they could allow managing large and spread tumors while limiting the duration and the invasiveness of the procedure. In this article, a grid electrode with 2-cm spaced needles has been studied by means of numerical models. The electroporation efficiency has been assessed on human osteosarcoma cell line MG63 cultured in monolayer. The computational results show the distribution of the electric field in a model of the treated tissue. These results are helpful to evaluate the effect of the needle distance on the electric field distribution. Furthermore, the in vitro tests showed that the grid electrode proposed is suitable to electropore, by a single application, a cell culture covering an area of 55 cm 2 . In conclusion, our data might represent substantial improvement in ECT in order to achieve a more homogeneous and time-saving treatment, with benefits for patients with cancer.

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“…To support the extracellular matrix in 3D culture, scaffold-based culture systems have been fabricated and shown to recreate the complex heterologous 3D structure of tumors in vitro (11)(12)(13)(14)(15). These 3D culture technologies have been utilized for the study of OS (13,(15)(16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional alginate spheroid culture system of murine osteosarcoma

Akeda

Nishimura²,

Satonaka³

et al. 2009

Oncol Rep

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Abstract. Osteosarcoma (OS) is the most common primary malignant tumor of the bone and often forms pulmonary metastases, which are the most important prognostic factor. For further elucidation of the mechanism underlying the progression and metastasis of human OS, a culture system mimicking the microenvironment of the tumor in vivo is needed. We report a novel three-dimensional (3D) alginate spheroid culture system of murine osteosarcoma. Two different metastatic clones, the parental Dunn and its derivative line LM8, which has a higher metastatic potential to the lungs, were encapsulated in alginate beads to develop the 3D culture system. The beads containing murine OS cells were also transplanted into mice to determine their metastatic potential in vivo. In this culture system, murine OS cells encapsulated in alginate beads were able to grow in a 3D structure with cells detaching from the alginate environment. The number of detaching cells was higher in the LM8 cell line than the Dunn cell line. In the in vivo alginate bead transplantation model, the rate of pulmonary metastasis was higher with LM8 cells compared with that of Dunn cells. The cell characteristics and kinetics in this culture system closely reflect the original malignant potential of the cells in vivo.

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MG‐63 human osteosarcoma cells grown in monolayer and as three‐dimensional tumor spheroids present a different metabolic profile: a ¹H NMR study

Cited by 51 publications

References 36 publications

Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Evaluation of the Electroporation Efficiency of a Grid Electrode for Electrochemotherapy

Three-dimensional alginate spheroid culture system of murine osteosarcoma

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MG‐63 human osteosarcoma cells grown in monolayer and as three‐dimensional tumor spheroids present a different metabolic profile: a 1H NMR study

Cited by 51 publications

References 36 publications

Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Evaluation of the Electroporation Efficiency of a Grid Electrode for Electrochemotherapy

Three-dimensional alginate spheroid culture system of murine osteosarcoma

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Product

Resources

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MG‐63 human osteosarcoma cells grown in monolayer and as three‐dimensional tumor spheroids present a different metabolic profile: a ¹H NMR study