MFS transportome of the human pathogenic yeast Candida albicans

Gaur, Manisha; Puri, Nidhi; Manoharlal, Raman; Rai, Versha; Mukhopadhayay, Gauranga; Choudhury, Devapriya; Prasad, Rajendra

doi:10.1186/1471-2164-9-579

Cited by 91 publications

(66 citation statements)

References 72 publications

(76 reference statements)

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“…In particular, the fact that excess unlabeled inositol did not decrease label incorporation from [ 3 H]inositol-GroPIns is evidence that our transport assays measure intact [ 3 H]inositolGroPIns transport and not the uptake of free [ 3 H]inositol following hydrolysis of [ 3 H]inositol-GroPIns outside the cell. If CaGit1 is a proton symporter, as predicted by in silico analysis (17), the protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP) should drastically reduce GroPIns transport activity, and it did (Fig. 6A).…”

Section: Resultsmentioning

confidence: 99%

“…Four ORFs with high similarity to ScGIT1, the gene that encodes the S. cerevisiae GroPIns and GroPCho transporter, have been identified in the C. albicans genome (4,17). CaGIT1 corresponds to orf19.34.…”

Section: Resultsmentioning

confidence: 99%

“…C. albicans contains four open reading frames (ORFs) (CaGIT1 to -4) predicted to encode transporters with a high degree of similarity to the S. cerevisiae GIT1 (ScGIT1) product (4). ScGit1 and CaGit1 to -4 are classified as members of the major facilitator superfamily (MFS) (17). The MFS is present in all kingdoms of life.…”

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confidence: 99%

“…Like ScGit1, CaGit1 to -4 are predicted to belong to the phosphate:H ϩ symporter (PHS) family (TC no. 2.A.1.9) of the MSF (17). In total, the C. albicans genome is predicted to have 5 PHS family members: CaGit1 to -4 and the homolog of the S. cerevisiae high-affinity phosphate transporter, Pho84.…”

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confidence: 99%

“…Those substrates include nutrients, drugs, nucleotides, nucleosides, and other metabolites (17,32). Using the Transport Commission (TC) system (37), 95 potential MFS proteins clustering into 17 families have been predicted for C. albicans (17), but only a handful have been characterized. Like ScGit1, CaGit1 to -4 are predicted to belong to the phosphate:H ϩ symporter (PHS) family (TC no.…”

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confidence: 99%

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Robust Utilization of Phospholipase-Generated Metabolites, Glycerophosphodiesters, by Candida albicans: Role of the CaGit1 Permease

et al. 2011

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Glycerophosphodiesters are the products of phospholipase-mediated deacylation of phospholipids. In Saccharomyces cerevisiae, a single gene, GIT1, encodes a permease responsible for importing glycerophosphodiesters, such as glycerophosphoinositol and glycerophosphocholine, into the cell. In contrast, the Candida albicans genome contains four open reading frames (ORFs) with a high degree of similarity to S. cerevisiae GIT1 (ScGIT1) Here, we report that C. albicans utilizes glycerophosphoinositol (GroPIns) and glycerophosphocholine (GroPCho) as sources of phosphate at both mildly acidic and physiological pHs. Insertional mutagenesis of C. albicans GIT1 (CaGIT1) (orf19.34), the ORF most similar to ScGit1, abolished the ability of cells to use GroPIns as a phosphate source at acidic pH and to transport

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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