Mevalonate kinase: Localization and variation in activity during the development of Sarcophaga bullata

Barnes, Francis J.; Goodfellow, Robert D.

doi:10.1016/0022-1910(71)90151-x

Cited by 17 publications

(2 citation statements)

References 17 publications

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“…The HMGR, predicted as an enzyme of the endoplasmic reticulum membrane in D. rhizophagus , has also been reported in the same site in mammals [56,57] and insects [58]. The prediction for MK, PMK and MDPC enzymes in D. rhizophagus agree with the cytosolic location [59,60] or in peroxisomes [61,62] reported in insects and humans. Lastly, the mitochondrial and cytoplasmic predictions for GPPS/FPPS and GGPPS enzymes in this bark beetle were in concordance with the findings for these enzymes in mammals [63,64] and Drosophila melanogaster [65].…”

Section: Discussionsupporting

confidence: 70%

The Differential Expression of Mevalonate Pathway Genes in the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) Is Unrelated to the de Novo Synthesis of Terpenoid Pheromones

Sarabia

López

Obregón-Molina

et al. 2019

IJMS

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Bark beetles commonly produce de novo terpenoid pheromones using precursors synthesized through the mevalonate pathway. This process is regulated by Juvenile Hormone III (JH III). In this work, the expression levels of mevalonate pathway genes were quantified after phloem feeding—to induce the endogenous synthesis of JH III—and after the topical application of a JH III solution. The mevalonate pathway genes from D. rhizophagus were cloned, molecularly characterized, and their expression levels were quantified. Also, the terpenoid compounds produced in the gut were identified and quantified by Gas Chromatography Mass Spectrometry (GC-MS). The feeding treatment produced an evident upregulation, mainly in acetoacetyl-CoA thiolase (AACT), 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), phosphomevalonate kinase (PMK), and isopentenyl diphosphate isomerase (IPPI) genes, and males reached higher expression levels compared to females. In contrast, the JH III treatment did not present a clear pattern of upregulation in any sex or time. Notably, the genes responsible for the synthesis of frontalin and ipsdienol precursors (geranyl diphosphate synthase/farnesyl diphosphate synthase (GPPS/FPPS) and geranylgeranyl diphosphate synthase (GGPPS)) were not clearly upregulated, nor were these compounds further identified. Furthermore, trans-verbenol and myrtenol were the most abundant compounds in the gut, which are derived from an α-pinene transformation rather than de novo synthesis. Hence, the expression of mevalonate pathway genes in D. rhizophagus gut is not directed to the production of terpenoid pheromones, regardless of their frequent occurrence in the genus Dendroctonus.

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Section: Discussionsupporting

confidence: 70%

The Differential Expression of Mevalonate Pathway Genes in the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) Is Unrelated to the de Novo Synthesis of Terpenoid Pheromones

Sarabia

López

Obregón-Molina

et al. 2019

IJMS

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“…Our experiments therefore took the form of a series of uninspired guesses. We attempted a few trials with mevalonic acid since it is an important intermediary in the biosynthesis of steroids and possibly also concerned with the synthesis of juvenile hormone (Barnes & Goodfellow, 1971). Although when introduced into tubes containing gravid fleas it undoubtedly boosted copulation, it certainly exerted no stimulating effect on maturation (Table IX(d)) comparable to that of nestling urine itself.…”

Section: Augmentioning

confidence: 99%

Factors influencing the breeding of the rabbit flea (Spilopsyllus cuniculi): A spring‐time accelerator and a kairomone in nestling rabbit urine with notes on Cediopsylla simplex, another “hormone bound” species

Rothschild¹,

Ford²

1973

Journal of Zoology

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Accelerated impregnation and maturation of the rabbit flea coincides with the spring peak litter‐size of the rabbit, but its breeding performance falls off before that of the domestic host. A kairomone nestling factor, which boosts copulation, impregnation and development of fleas feeding on the newborn young, is also present in relatively feeble amounts in baby rabbit urine. This urine boosts pairing of matured (=“primed”) fleas united in tubes away from a host, but impregnation is increased only if fleas are feeding on newborn young while exposed to the urine. Undeveloped fleas matured on the pregnant doe require a transfer to another host before they will pair, whereas fleas matured on the nestlings copulate without a transfer. A variable time‐lag between transfer and copulation of fleas, dependent on the “priming” qualities of the preparatory host, suggests that a chemical intermediary is involved. If the antennae of the females are coated with vaseline, the maturation of these fleas is delayed feeding on nestlings, but not on the prepartum doe. This delay is less marked during the spring acceleration than at other times of the year. The breeding of a North American species of hare flea (Cediopsyllu simplex (Baker)) parasitizing Sylvilagus floridanus (Allen) is also controlled by the sex hormones of the host.

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Diptera

Singh¹

1977

Artificial Diets for Insects, Mites, and Spiders

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Mevalonate kinase: Localization and variation in activity during the development of Sarcophaga bullata

Cited by 17 publications

References 17 publications

The Differential Expression of Mevalonate Pathway Genes in the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) Is Unrelated to the de Novo Synthesis of Terpenoid Pheromones

The Differential Expression of Mevalonate Pathway Genes in the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) Is Unrelated to the de Novo Synthesis of Terpenoid Pheromones

Factors influencing the breeding of the rabbit flea (Spilopsyllus cuniculi): A spring‐time accelerator and a kairomone in nestling rabbit urine with notes on Cediopsylla simplex, another “hormone bound” species

Diptera

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