Metronidazole release using natural rubber latex as matrix

Abstract

Natural Rubber Latex (NRL) can be used successfully in controlled release drug delivery due to their excellent matrix forming properties. Recently, NRL has shown to stimulate angiogenesis, cellular adhesion and the formation of extracellular matrix, promoting the replacement and regeneration of tissue. A dermatological delivery system comprising a topically acceptable, inert support impregnated with a metronidazole (MET) solution was developed. MET 2-(2-methyl-5-nitro-1H-imidazol-1-yl) ethanol, has been widely used for the treatment of protozoa and anaerobic bacterial infections. MET is a nitroimidazole anti-infective medication used mainly in the treatment of infections caused by susceptible organisms, particularly anaerobic bacteria and protozoa. In a previous study, we have tested NRL as an occlusive membrane for GBR with promising results. One possible way to decrease the inflammatory process, it was incorporated the MET in NRL. MET was incorporated into the NRL, by mixing it in solution for in vitro protein delivery experiments. The solutions of latex and MET were polymerized at different temperatures, from -100 to 40 °C, in order to control the membrane morphology. SEM microscopy analysis showed that the number, size and distribution of pores in NRL membranes varied depending on polymerization temperature, as well as its overall morphology. Results demonstrated that the best drug-delivery system was the membrane polymerized at -100 °C, which does release 77,1% of its MET content for up 310 hours.

Evaluation of sodium diclofenac release using natural rubber latex as carrier

“…We can observed that diclofenac is encapsulated in natural latex membranes, where the NRL membrane allows a gradual release of diclofenac from the aqueous solution. We observed that the release profiles for sodium diclofenac in a NRL matrix is similar from our previous work 8 , but the saturation point is different and the inclination of bi-exponential function. In this work, the points to saturation at approximately 74 hours.…”

“…Herculano et al 7,8,35 proposed a drug release system based on natural rubber latex (NRL) for the sustained and controlled delivery of metronidazole (MET), Bovine Serum Albumine (BSA) and Stryphnodendron sp extract. They concluded that the release time of MET, BSA and Stryphnodendron sp in in vitro tests were very promising for the kinetics of release.…”

“…After extraction, ammonia was used to keep the latex liquid. NRL was performed by centrifugation at 8,000 g [7,8] to reduce the protein content related with allergies. Sodium diclofenac (C 14 H 11 Cl 2 NO 2 ) was purchased from CALLITHEA Pharmaceutics Ltd., Brazil.…”

“…One candidate already described in literature [7][8][9][10][11] as a potential carrier is the natural rubber latex (NRL) from Hevea brasiliensis. The NRL membrane is an important inductor of the healing process of wounds, being used in several medical applications like prosthetics and bone grafts [12][13][14][15][16] .…”

Development Assessment of Natural Latex Membranes: A New Proposal for the Treatment of Amblyopia

“…The membranes were obtained by deposition on the petri dish as described previously 16,17,18 . The transparency of the membrane was favored by the use of room temperature.…”