Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation

Abstract

The aim of this investigation was to monitor metronidazole concentrations in the gingival crevicular fluid (GCF) collected from periodontal pockets of dogs after treatment with an experimental 15% metronidazole gel. Five dogs had periodontitis induced by cotton ligatures placed subgingivally and maintained for a 30-day period. After the induction period, only pockets with 4 mm or deeper received the gel. Each pocket was filled up to the gingival margin by means of a syringe with a blunt-end needle. GCF was collected in paper strips and quantified in an electronic device before and after 15 minutes, 1 h, 6 h, 24 h and 48 h of gel administration. The GCF samples were assayed for metronidazole content by means of a high performance liquid chromatography method. Concentrations of metronidazole in the GCF of the 5 dogs (mean ± SD, in µg/mL) were 0 ± 0 before gel application and 47,185.75 ± 24,874.35 after 15 minutes, 26,457.34 ± 25,516.91 after 1 h, 24.18 ± 23.11 after 6 h, 3.78 ± 3.45 after 24 h and 3.34 ± 5.54 after 48 h. A single administration of the 15% metronidazole gel released the drug in the GCF of dogs in levels several-fold higher than the minimum inhibitory concentration for some periodontopathogens grown in subgingival biofilms for up to one hour, but metronidazole could be detected in the GCF at least 48 hours after the gel application.

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

“…For treating periodontitis, a number of resorbable drug delivery systems were developed during recent decades, such as drug loaded hydroxypropyl cellulose films [8], or drug carrying gels such as Elyzol® (Dumex GmbH, Bad Vilbel, Germany) dental gel, based on melted glycerol mono-oleate [9,10]. However, for these systems, the periodontal milieu often poses the major problem that the required period of drug exposure (7-10 days) cannot be achieved [11].…”

Effect of Cassave leaf flavonoid extract on TNF-α expressions in rat models suffering from periodontitis

“…The gel was applied twice a day for 7 days. This procedure was based on a procedure proposed by Sato et al 16 The exceess gel on the gingival sulcus then was cleaned with a cotton pellet.…”

Topical drug delivery: An essential aid in the management of oral diseases

“…The local delivery of metronidazole gel is advantageous because of the ability of releasing antimicrobial agents in concentrations high enough to affect pathogens, even in subgingival biofilms. [38] Stellini et al verified efficacy of metronidazole dental gel 25%, used as a topical antibiotic for the treatment of peri-implantitis and found that peri-implant diseases can be positively resolved with 60-70% decrease of Gram-negative and 40-50% increase in Gram-positive organisms and a good peri-implant soft tissue recovery. [39,40] …”