Metric between buckling structures and elastic properties in physisorbed polymer-tethered lipid monolayers

Siegel, Amanda P.; Hussain, Noor F.; Johnson, Merrell A.; Naumann, Christoph

doi:10.1039/c2sm25150g

Cited by 8 publications

(25 citation statements)

References 48 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At low lipopolymer concentrations (low lateral stress), polymer-tethered monolayers and bilayers typically lack substantial buckling formation. In contrast, at medium to high lipopolymer content (high lateral stress), buckling structures can be observed, which become more prominent in terms of buckling width and bearing area (percentage of buckling region), with increasing lipopolymer molar concentration [16]. Interestingly, the EPI micrographs in Figure 1A-C demonstrate that membrane buckling in polymer-tethered membranes can also be caused by varying concentrations of CHOL.…”

Section: Resultsmentioning

confidence: 92%

“…Importantly, their membrane elasticity can be adjusted by the molar concentration of lipopolymers in the bottom leaflet of the bilayer [16]. As reported before, the presence of elevated concentrations of membrane stress-imparting lipopolymers may lead to a stress relaxation processes, such as buckling delamination of the membrane on solid substrates [15].…”

Section: Resultsmentioning

confidence: 99%

“…1) were purchased from VWR Scientific Products (West Chester, PA, USA). Glass coverslips were cleaned before usage, as described previously [16].…”

Section: Methodsmentioning

confidence: 99%

“…Previously, lipid monolayers with lung surfactant proteins have been successfully utilized to explore biophysical mechanisms associated with the reversible lipid monolayer collapse during the breathing cycle of a lung [8,[12][13][14]. Recently, our group reported that physisorbed polymer-tethered membranes containing phospholipids, and lipopolymers represent another model membrane system, in which membrane buckling phenomena can be investigated under well-controlled conditions [15,16]. In this model system, the elastic properties can be modified simply through adjustment of lipopolymer molar concentration in the membrane [17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Here, the bilayer compartmentalization was attributed to a stress relaxation effect, which is associated with the partial penetration of polymer moieties of lipopolymers into the hydrophobic region of the bottom monolayer, thus preventing bilayer formation at buckling regions. More recently, we also derived a metric between membrane elasticity and quantifiable buckling parameters, w max and b, in physisorbed mixed monolayers of poly(ethylene glycol) (PEG) lipopolymers and phospholipids by combining mean-field calculations of polymer-tethered membranes and buckling theory for a straight-sided blister [16]. Notably, physisorbed polymer-tethered lipid bilayers, which are compartmentalized by buckling structures, show fascinating length scale-dependent lipid diffusion properties with remarkable parallels to those found in plasma membranes [19].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Cholesterol-Induced Buckling in Physisorbed Polymer-Tethered Lipid Monolayers

et al. 2013

Self Cite

View full text Add to dashboard Cite

Abstract:The influence of cholesterol concentration on the formation of buckling structures is studied in a physisorbed polymer-tethered lipid monolayer system using epifluorescence microscopy (EPI) and atomic force microscopy (AFM). The monolayer system, built using the Langmuir-Blodgett (LB) technique, consists of 3 mol % poly(ethylene glycol) (PEG) lipopolymers and various concentrations of the phospholipid, 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC), and cholesterol (CHOL). In the absence of CHOL, AFM micrographs show only occasional buckling structures, which is caused by the presence of the lipopolymers in the monolayer. In contrast, a gradual increase of CHOL concentration in the range of 0-40 mol % leads to fascinating film stress relaxation phenomena in the form of enhanced membrane buckling. Buckling structures are moderately deficient in CHOL, but do not cause any notable phospholipid-lipopolymer phase separation. Our experiments demonstrate that membrane buckling in physisorbed polymer-tethered membranes can be controlled through CHOL-mediated adjustment of membrane elastic properties. They further show that CHOL may have a notable impact on molecular confinement in the presence of crowding agents, such as lipopolymers. Our results are significant, because they offer an intriguing prospective on the role of CHOL on the material properties in complex membrane architecture. OPEN ACCESSPolymers 2013, 5 405

show abstract

Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

“…1) were purchased from VWR Scientific Products (West Chester, PA, USA). Glass coverslips were cleaned before usage, as described previously [16].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cholesterol-Induced Buckling in Physisorbed Polymer-Tethered Lipid Monolayers

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

Can We Rebuild the Cell Membrane?

Damiati

2018

Biological, Physical and Technical Basics of Cell Engineering

View full text Add to dashboard Cite

Tunable cell-surface mimetics as engineered cell substrates

Shilts

Naumann

2018

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

Most recent breakthroughs in understanding cell adhesion, cell migration, and cellular mechanosensitivity have been made possible by the development of engineered cell substrates of well-defined surface properties. Traditionally, these substrates mimic the extracellular matrix (ECM) environment by the use of ligand-functionalized polymeric gels of adjustable stiffness. However, such ECM mimetics are limited in their ability to replicate the rich dynamics found at cell-cell contacts. This review focuses on the application of cell surface mimetics, which are better suited for the analysis of cell adhesion, cell migration, and cellular mechanosensitivity across cell-cell interfaces. Functionalized supported lipid bilayer systems were first introduced as biomembrane-mimicking substrates to study processes of adhesion maturation during adhesion of functionalized vesicles (cell-free assay) and plated cells. However, while able to capture adhesion processes, the fluid lipid bilayer of such a relatively simple planar model membrane prevents adhering cells from transducing contractile forces to the underlying solid, making studies of cell migration and cellular mechanosensitivity largely impractical. Therefore, the main focus of this review is on polymer-tethered lipid bilayer architectures as biomembrane-mimicking cell substrate. Unlike supported lipid bilayers, these polymer-lipid composite materials enable the free assembly of linkers into linker clusters at cellular contacts without hindering cell spreading and migration and allow the controlled regulation of mechanical properties, enabling studies of cellular mechanosensitivity. The various polymer-tethered lipid bilayer architectures and their complementary properties as cell substrates are discussed.

show abstract

Metric between buckling structures and elastic properties in physisorbed polymer-tethered lipid monolayers

Cited by 8 publications

References 48 publications

Cholesterol-Induced Buckling in Physisorbed Polymer-Tethered Lipid Monolayers

Cholesterol-Induced Buckling in Physisorbed Polymer-Tethered Lipid Monolayers

Can We Rebuild the Cell Membrane?

Tunable cell-surface mimetics as engineered cell substrates

Contact Info

Product

Resources

About