Métodos de inoculação para quantificação de resistência em soja a Fusarium solani f. sp. glycines, em casa-de-vegetação

Klingelfuss, L. H.; Yorinori, J. T.; Destro, Deonisio

doi:10.1590/s0100-41582007000100007

Cited by 11 publications

(9 citation statements)

References 17 publications

(27 reference statements)

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“…Then, four discs of approximately 5 mm in diameter, containing mycelium and conidia of the fungus were added in erlenmeyers flasks containing corn kernels. The flasks were incubated at 25 ± 2 °C, with photoperiod of 12 hours until use (KLINGELFUSS et al, 2007). Prior to transplanting the seedlings, the substrate was sterilized twice at 120 °C and 1 atm for 1 hour, with a 24 hour interval.…”

Section: Methodsmentioning

confidence: 99%

“…The use of biological control of soilborne pathogens in floriculture has the potential to be widely diffused, as it is a method that presents lower cost and does not cause contamination to the environment (LECOMTE et al, 2016). In this sense, the use of fungi of the genus Trichoderma has been increasing in the last years, mainly for the ability of these antagonists to control a range of phytopathogenic fungi in diverse cultures.…”

Section: Variables -------------------Treatments-------------------mentioning

confidence: 99%

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Evaluation of biological control of fusarium wilt in gerbera with Trichoderma asperellum

Brandler

Divensi

Tonin

et al. 2017

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The increase in flower cultivation in recent years has been reflecting the higher incidence of soil pathogens that can cause serious problems. This study aimed to evaluate the biological control of Fusarium wilt in gerbera with Trichoderma asperellum. The evaluated treatments were: T1) Control, only sterile substrate; T2) Substrate + Fusarium oxysporum; T3) Substrate + Fusarium oxysporum + Trichoderma asperellum; and T4) Substrate + Trichoderma asperellum. For this, the pathogen was isolated from gerbera with disease symptoms and, subsequently, it was identified according to morphological characters. Furthermore, the degree of antagonism of T. asperellum against F. oxysporum was evaluated through the culture pairing test. For greenhouse evaluations, commercial autoclaved substrate was used and infested with corn grains infected by the pathogen. Morphological identification confirmed the pathogen species as Fusarium oxysporum. In the culture pairing test, it was found that T. asperellum did not present a high degree of antagonism. The plants cultivated on substrate infested by the pathogen had no visible symptoms of wilt, but the substrate infestation with the pathogen provided lower values of fresh and dry mass of shoots and roots. The treatment with T. asperellum obtained higher values of fresh and dry mass of both shoots and roots, and also more vigorous inflorescences in relation to the plants treated with the pathogen

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Section: Methodsmentioning

confidence: 99%

Section: Variables -------------------Treatments-------------------mentioning

confidence: 99%

Evaluation of biological control of fusarium wilt in gerbera with Trichoderma asperellum

Brandler

Divensi

Tonin

et al. 2017

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“…a) Inoculation of Fusarium spp in maize kernels: maize kernels were soaked in water and incubated at room temperature for 8 h, after which excess water was discarded and approximately 80 g kernels were placed in 100-mL glass flasks to be autoclaved twice for 40 min at an interval of 24 h. After cooling, 5 5-mm-diameter PDA culture medium discs containing pathogen mycelia were transferred to each vial. The flasks were incubated at 24°C for 14 days with 12-h light/dark cycles according to a method adapted from Klingelfuss et al (2007). Five PDA culture medium discs not containing pathogens were used as controls.…”

Section: Pathogenicity Testmentioning

confidence: 99%

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Lazarotto¹,

Milanesi²,

Muniz³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The occurrence of Fusarium spp associated with pecan tree (Carya illinoinensis) diseases in Brazil has been observed in recent laboratory analyses in Rio Grande do Sul State. Thus, in this study, we i) obtained Fusarium isolates from plants with disease symptoms; ii) tested the pathogenicity of these Fusarium isolates to pecan; iii) characterized and grouped Fusarium isolates that were pathogenic to the pecan tree based on morphological characteristics; iv) identified Fusarium spp to the species complex level through TEF-1α sequencing; and v) compared the identification methods used in the study. Fifteen isolates collected from the inflorescences, roots, and Fusarium spp pathogenic to pecan tree seeds of symptomatic plants (leaf necrosis or root rot) were used for pathogenicity tests. Morphological characterization was conducted using only pathogenic isolates, for a total of 11 isolates, based on the mycelial growth rate, sporulation, colony pigmentation, and conidial length and width variables. Pathogenic isolates were grouped based on morphological characteristics, and molecular characterization was performed by sequencing TEF-1α genes. Pathogenic isolates belonging to the Fusarium chlamydosporum species complex, Fusarium graminearum species complex, Fusarium proliferatum, and Fusarium oxysporum were identified based on the TEF-1α region. Morphological characteristics were used to effectively differentiate isolates and group the isolates according to genetic similarity, particularly conidial width, which emerged as a key morphological descriptor in this study.

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“…After cooling, five PDA culture medium discs (7 mm in diameter), containing the pathogen mycelium, were transferred to each flask. The flasks were incubated at 24 °C, with 12 h of photoperiod, during 14 days, according to methodology of Klingelfuss et al (15) modified by Lazarotto et al (16). For the control, five discs of culture medium without the pathogen were placed in flasks, and the other procedures were performed as described previously.…”

Section: Methodsmentioning

confidence: 99%

Aggressiveness of Fusarium oxysporum and Fusarium solani isolates to yerba-mate and production of extracellular enzymes

et al. 2019

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The yerba-mate (Ilex paraguariensis) has great socioeconomic importance on family farming in Southerm Brazil. One of the main yerba-mate disease is root rotting, caused by Fusarium spp. Little is known about the pathogen physiology, especially regarding the aggressiveness associated with the production of extracellular enzymes. On this work, the aggressiveness of isolates of F. oxysporum and F. solani pathogenic to yerba-mate was evaluated and it was determined the activities of extracellular enzymes catalase, laccase, cellulase, caseinase, amylase, protease, lipase and pectinases produced by Fusarium spp. in culture medium. Six isolates of F. solani and one isolate of F. oxysporum pathogenic to yerba-mate were used. The F. oxysporum isolate proved to be less aggressive in relation to the other F. solani isolates. All isolates of Fusarium spp. produced, on a semiquantitative manner, the extracellular enzymes catalase, laccase, cellulase, caseinase, amylase, protease, lipase and pectinases (polygalacturonase and pectate lyase). However, the quantity produced for each enzyme was significantly different among the isolates. With the exception of the laccase and polygalacturonase enzymes, the M7C1 isolate showed the highest enzymatic index and was also responsible for the highest percentage of yerba-mate seedlings death.

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Métodos de inoculação para quantificação de resistência em soja a Fusarium solani f. sp. glycines, em casa-de-vegetação

Cited by 11 publications

References 17 publications

Evaluation of biological control of fusarium wilt in gerbera with Trichoderma asperellum

Evaluation of biological control of fusarium wilt in gerbera with Trichoderma asperellum

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Aggressiveness of Fusarium oxysporum and Fusarium solani isolates to yerba-mate and production of extracellular enzymes

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