Metode Ekstraksi Dna Untuk Deteksi Molekuler

Hutami, Rosy; Idzni, N; Ranasasmita, Raafqi; Suprayatmi, Mira

doi:10.30997/jp.v8i2.1056

Cited by 3 publications

(4 citation statements)

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“…Metode ekstraksi phenol-chloroform merupakan metode ektraksi fase cair (Hutami et al 2017). Metode ekstraksi phenol-chloroform secara umum terdiri dari tiga tahap yakni proses lisis sel, pemisahan DNA dan pemurnian DNA.…”

Section: Pendahuluanunclassified

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Ekstraksi DNA dari Daging Segar untuk Analisis dengan Metode Loop-Mediated Isothermal Amplification (LAMP)

Hutami

Bisyri

Sukarno

et al. 2018

JAH

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DNA extraction is needed in the analysis using the Loop-mediated isothermal amplification (LAMP) method because this method identifies nucleic acids. Some extraction methods that can be selected including commercial kits extraction method and phenol-chloroform extraction method. The purpose of this study was to obtain the best quality DNA extract between the two extraction methods. The DNA extraction process produced DNA concentrations between 31.06 - 410.18 ng / ml for the commercial kit DNA extract and 212.60 - 1502.30 ng / ml for the phenol-choroform DNA extract, while the purity of DNA were 1.82-2.02 for commercial kit DNA extract and 1.93-2.02 for phenol-chloroform DNA extract. The concentration and purity of extracts produced from both methods meet the requirements for molecular analysis. The purity and visualization results of commercial kit DNA extract are better than those produced from extraction from the phenol-chloroform method. DNA extract obtained from the commercial kit method was chosen to be used in the amplification stage of the method (LAMP).

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Section: Pendahuluanunclassified

“…Penambahan fenol menyebabkan protein kehilangan kelarutannya dan mengalami presipitasi sehingga selanjutnya dapat dipisahkan dari DNA melalui sentrifugasi (Karp 2008). Larutan fenol dan CIA dapat mengikat protein, sebagian kecil RNA, lipid dan polisakarida (Sambrook et al 1989 (Hutami et al 2017).…”

Section: Pendahuluanunclassified

Ekstraksi DNA dari Daging Segar untuk Analisis dengan Metode Loop-Mediated Isothermal Amplification (LAMP)

Hutami

Bisyri

Sukarno

et al. 2018

JAH

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“…Microplastics are floating in the water and accidentally consumed by fish that are looking for food. It accumulates in the fish's body and causes physical and chemical damage such as damage to internal organs and blockage of the digestive tract, as carcinogenic, and endocrine disorders [3,4]. The primary reaction, secondary response, and tertiary response are the three categories into which fish response mechanisms to stressors in the body are separated [4].…”

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“…It accumulates in the fish's body and causes physical and chemical damage such as damage to internal organs and blockage of the digestive tract, as carcinogenic, and endocrine disorders [3,4]. The primary reaction, secondary response, and tertiary response are the three categories into which fish response mechanisms to stressors in the body are separated [4]. The brain directly receives the primary responses that take place when fish are under stress.…”

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Dumbo Catfish (Clarias Gariepinus) Microplastic Exposure on Abnormalities and Level of Blood Component Using a Completely Randomized Design

Mangkunegara,

Fadjar,

Iranawati

2023

Jurnal Teknologi

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Recently, the increase in environmental pollution has been studied by researchers, especially the increase in abnormalities and blood level in aquatic organisms. In this regard, one of the reasons is the presence of plastic causes that are often found in water. The existence of plastic waste in the water is divided into 3 categories, namely macroplastic (>25 mm), mesoplastic (5-25 mm), and microplastic (<5 mm). Microplastics occur from the degradation of macroplastics and mesoplastics in waters. Microplastics in lakes, rivers, and estuaries cause a stress response in the bodies of aquatic organisms including dumbo catfish (Claries gariepinus). The stress response of fish can be seen in the changes in blood components and levels of blood, which are used to determine the condition or health status of fish by counting the number of erythrocytes, leucocytes, hemoglobin, and differential leucocytes. This study aims to analyze the stress response of dumbo catfish caused by microplastic exposure to hematology. This used research method was a completely randomized design (CRD) with one factor and three treatment levels. The results showed that the increase in rearing time was from 0 to 30 days and varied of doses mixture commercial fish feed and microplastic. The results showed the highest erythrocyte value of 2.78(x106) cell/mm3 in 15 days of rearing time by adding mixed commercial feed and microplastics of 15%.

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A CONTRAST PATTERN OF REEF FISH SPECIES DIVERSITY AND DISTRIBUTION USING ENVIRONMENTAL DNA (eDNA) METABARCODING IN LONGITUDINAL DISTANCE FROM JAKARTA BAY

Sani¹,

Benyamin²,

Husna³

et al. 2021

J. Ilmu dan Teknologi Kelautan Tropis

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The existence of reef fish is certainly closely related to the existence of reefs coral because the ecosystem is a habitat for reef fish. Coral reefs are ecosystems that are commonly found on small islands in the tropics including the Seribu Islands. The Seribu Islands are a group of 110 islands located off the coast of Jakarta and up to 80 kilometers north of the Java Sea. In this study, we examined the species distribution and diversity of reef fish species on two different distance location in Jakarta Bay using environmental DNA (eDNA) metabarcoding analysis from two sites which are Untung Jawa Island and Harapan Island. The 4L eDNA seawater samples were collected at a depth of 8-9 meters at each site and then analysis using specific primer (MiFish U) of 12S rRNA. Overall, the higher species richness was found on Harapan Island (52 species) followed by Untung Jawa Island (11 species). The Shannon-Wiener Index also showed Harapan Island has higher reef fish diversity based on three taxonomic level (family, genus, and species). There were only five mutual reef fish species found in the two locations, namely Atherinomorus aetholepis, Auxis thazard, Cephalopholis sexmaculata, Epinephelus chlorostigma, and Plectropomus areolatus. The results of these findings in this current study are in line with anthropogenic pressure different where Untung Jawa Island is the closer one to Jakarta Bay than the Harapan Island that located relatively far from Jakarta Bay.

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Metode Ekstraksi Dna Untuk Deteksi Molekuler

Cited by 3 publications

References 0 publications

Ekstraksi DNA dari Daging Segar untuk Analisis dengan Metode Loop-Mediated Isothermal Amplification (LAMP)

Ekstraksi DNA dari Daging Segar untuk Analisis dengan Metode Loop-Mediated Isothermal Amplification (LAMP)

Dumbo Catfish (Clarias Gariepinus) Microplastic Exposure on Abnormalities and Level of Blood Component Using a Completely Randomized Design

A CONTRAST PATTERN OF REEF FISH SPECIES DIVERSITY AND DISTRIBUTION USING ENVIRONMENTAL DNA (eDNA) METABARCODING IN LONGITUDINAL DISTANCE FROM JAKARTA BAY

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