2018
DOI: 10.1016/j.scr.2018.07.020
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Meticulous optimization of cardiomyocyte yields in a 3-stage continuous integrated agitation bioprocess

Abstract: Human pluripotent stem cells (hPSCs) can be a renewable source for generating cardiomyocyte (CM) for treating myocardial infraction. In our previous publication, we described an integrated microcarrier-based wave reactor process for the expansion and differentiation of hPSCs to CMs on a rocker based platform. However, this platform is limited in terms of linear scalability and CMs purity. The present study describes ways to overcome these limitations by the use of a stirred scalable platform and incorporation … Show more

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Cited by 14 publications
(34 citation statements)
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“…Method for microcarrier coating have been reported earlier by our group [57,58]. Briefly, 20 mg/mL of Cytodex 1 (GE Healthcare, USA) was coated with (1:30 v/v) Geltrex® in DMEM/F12 medium (Thermo Fisher Scientific) overnight at 4°C.…”
Section: Preparation Of Microcarriersmentioning
confidence: 99%
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“…Method for microcarrier coating have been reported earlier by our group [57,58]. Briefly, 20 mg/mL of Cytodex 1 (GE Healthcare, USA) was coated with (1:30 v/v) Geltrex® in DMEM/F12 medium (Thermo Fisher Scientific) overnight at 4°C.…”
Section: Preparation Of Microcarriersmentioning
confidence: 99%
“…Besides, although EBs with controlled size can be form by many methods [45,46,62], they might be limited to research scale [37]. In the past few years, our group has developed protocols for scale-up and production of de novo hPSC-/hiPSC-derived CMs based on the use of MC cultures in platforms such as rockers and spinners [7,8,34,35,38,[57][58][59]. However, in order to generate high-quantity and high-quality CMs, applying intermittent agitation during the first 3 days of cardiac differentiation phase is important [57,58], which makes it problematic in large-scale reactors.…”
Section: Introductionmentioning
confidence: 99%
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