Methylprednisolone induces apoptosis by interacting with the JAK/STAT pathway in HL-60 and K-562 leukemic cells

Kaymaz, Burçin Tezcanlı; Selvi, Nur; Saydam, Güray; Şahin, Fahri; Kosova, Buket

doi:10.1179/102453312x13221316477859

Cited by 11 publications

(7 citation statements)

References 21 publications

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“…The regulation of various extracellular factors on cell behavior is closely related to the activation of intracellular multiple signaling transduction pathways. Among them, activation of the JAK/STAT pathway in cell apoptosis has been widely studied in recent years (26–28). The JAK/STAT signaling pathway is one of the important pathways of cytokine transduction and can play an important regulatory role in cellular physiological and pathological reactions.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B virus X protein modulates apoptosis in human renal proximal tubular epithelial cells by activating the JAK2/STAT3 signaling pathway

Zhang

et al. 2013

International Journal of Molecular Medicine

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Hepatitis B virus X protein (HBx) is a multifunctional protein, and it activates multiple signal transduction pathways in multiple types of cells and regulates the process of cell apoptosis. In the present study, we mainly investigated the correlation between HBx and renal tubular epithelial cell apoptosis in hepatitis B virus-associated glomerulonephritis (HBVGN) and the possible signaling mechanism. Cell apoptosis in nephridial tissues of patients with HBVGN were determined by the TUNEL method. HBx, p-STAT3 and STAT3 levels in nephridial tissues were determined by immunohistochemical assay, and a correlation analysis between HBx expression levels and apoptosis index in nephridial tissues was conducted. The activation of the JAK2/STAT3 signaling pathway in HK-2 cells and the expression of the apoptosis-related proteins Bax and Bcl-2 were determined by western blot analysis following transfection with the HBx eukaryotic expression vector. Cellular proliferation activity was determined by the CCK-8 method, and cell apoptosis was determined with HO33342 staining using transmission electron microscopy and Annexin V/PI double staining flow cytometry. The results revealed that the apoptosis index in nephridial tissues of patients with HBVGN was significantly higher when compared to that of the control group, and p-STAT3 expression levels in HBVGN nephridial tissues were significantly increased. In the control group, no HBx expression was observed in the nephridial tissues, whereas HBx expression was found in the nephridial tissues of 86% of the patients with HBVGN. The HBx expression levels had a linear correlation with the apoptosis index in the nephridial tissues. After target gene HBx infection, expression levels of both p-JAK2 and p-STAT3 in human proximal HK-2 cells were significantly increased, and the Bax/Bcl-2 ratio was also significantly increased. At the same time, cellular proliferation of HK-2 cells was significantly inhibited, and the rate of apoptosis was increased. After incubation with AG490, the JAK2/STAT3 signaling pathway was partially blocked, which caused a decrease in the Bax/Bcl-2 ratio and reduced cell apoptosis caused by HBx. In conclusion, HBx upregulates the Bax/Bcl-2 ratio by activating the JAK2/STAT3 signaling pathway to cause renal tubular epithelial cell apoptosis, and it is possibly involved in the pathogenic mechanism of nephridial tissue damage caused by HBV.

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Section: Discussionmentioning

confidence: 99%

Hepatitis B virus X protein modulates apoptosis in human renal proximal tubular epithelial cells by activating the JAK2/STAT3 signaling pathway

Zhang

et al. 2013

International Journal of Molecular Medicine

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“…There, these complexes bind to specific DNA sequences and alter their expression. In particular, there is good evidence that the IL-6/JAK/STAT3 pathway in tumor cells can be downregulated by glucocorticoids (eg, Kaymaz et al 26 ).…”

Section: Targeting Innate/humoral Immune/ Inflammatory Responsesmentioning

confidence: 98%

Therapeutics targeting innate immune/inflammatory responses through the interleukin-6/JAK/STAT signal transduction pathway in patients with cancer

Roxburgh

McMillan

2016

Translational Research

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“…It has been showed that K562 when treated with curcumin inhibited Jak2 mRNA expression and down regulated the activity of STAT5a (Blasius et al, 2006). Similarly, a significant decrease was seen in STAT5A mRNA relative expression level at 48 hours of Methylprednisolone treatment (P<0.05) in K-562 cells (Kaymaz et al, 2012). When penicillin treatment was given K562, STAT5A expression was down regulated.…”

Section: Discussionmentioning

confidence: 80%

Inhibition of Proliferation of Cervical and Leukemic Cancer Cells by Penicillin G

Banerjee¹,

Dahiya²,

Anand³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Cancer, despite all the efforts, still causes one in five deaths worldwide. Surgery, chemotherapy and radiotherapy provide inadequate protection and instead affect normal cells along with cancer cells. The search for cancer cures from natural products (plants and animals) has been practice for over a decade and the use of purified chemical to treat cancer still continues. Several studies have been undertaken during last three decades to find the anti-cancerous property of various plant extract and toxins secreted by animals and micro-organism. These lead to the discovery of several promising molecule having anticancer activity, some of which are in clinical trial and may emerged to be a potential future drug in cancer therapy. In this study we have used penicillin to evaluate its anti-cancer activity. It shown significant effects at cellular and molecular levels against growth of HeLa and K562 cell lines.

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Methylprednisolone induces apoptosis by interacting with the JAK/STAT pathway in HL-60 and K-562 leukemic cells

Cited by 11 publications

References 21 publications

Hepatitis B virus X protein modulates apoptosis in human renal proximal tubular epithelial cells by activating the JAK2/STAT3 signaling pathway

Hepatitis B virus X protein modulates apoptosis in human renal proximal tubular epithelial cells by activating the JAK2/STAT3 signaling pathway

Therapeutics targeting innate immune/inflammatory responses through the interleukin-6/JAK/STAT signal transduction pathway in patients with cancer

Inhibition of Proliferation of Cervical and Leukemic Cancer Cells by Penicillin G

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