Methylophaga marina gen. nov., sp. nov. and Methylophaga thalassica sp. nov., Marine Methylotrophs

Characterization of Species of Marine Methylotrophs of the Genus Methylophaga

“…Differences among these results may be ascribed to cultural conditions or unknown factors, and utilization of D-fructose and reduction of nitrate do not always give clear-cut results. The DNA base composition of the genus Methylophaga was reported to be between 38 and 46 mol% G+C by Janvier et al (7). This range is a little different from our results (43.3 to 48.7 mol%) and those of Yamamoto et al (43.8 to 47.6 mol% (28).…”

“…The generic name "Methylophilus" was given to the obligately methanol-utilizing, polarly flagellated, gramnegative bacteria by Loginova and Trotsenko (9), but this genus has not been validated to date. In 1985, Janvier et al (7) studied the taxonomic characteristics of these marine obligate methylotrophs and proposed a new genus, Methylophaga, with two new species, Methylophaga marina and Methylophaga thalassica. These species were principally established on the basis of deoxyribonucleic acid (DNA)-DNA hybridization data and the electrophoretic patterns of enzymes.…”

Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

“…Sequences related to Methylophaga alcalica, a haloalkaliphilic methylotroph isolated from sediments of an East Mongolian soda lake (Doronina et al, 2003b), were recovered from the mxaF clone library. Methylophaga are aerobic, moderately halophilic, nonmethane using methylotrophs, mostly isolated from marine (Janvier et al, 1985;Doronina et al, 1997;Kim et al, 2007) and soda lake ecosystems (Doronina et al, 2003a(Doronina et al, , 2003b. 16S rRNA and mxaF gene sequences related to the alkaline environment isolate Methylomicrobium sp.…”

Phylogenetic position and emended description of the genus Methylovorus

“…To date, four genera have been described for the group of obligately and restricted facultatively methylotrophic bacteria (methylobacteria) with the ribulose monophosphate (RuMP) pathway of C 1 assimilation: Methylophaga (Janvier et al, 1985;de Zwart et al, 1996;Doronina et al, 2003a, b), Methylobacillus (Yordy & Weaver, 1977;Urakami & Komagata, 1986), Methylophilus (Jenkins et al, 1987) and Methylovorus (Govorukhina & Trotsenko, 1991).…”

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The genus Methylovorus, currently represented by the restricted facultative methylotroph Methylovorus glucosotrophus Govorukhina and Trotsenko 1991 and the obligate methylotroph Methylovorus mays Doronina et al 2001, is here established by direct sequencing of amplified 16S rRNA genes and DNA-DNA hybridization to be clearly separated from the extant ribulose monophosphate (RuMP) pathway methylobacteria and to form a distinct branch within the b-Proteobacteria.To date, four genera have been described for the group of obligately and restricted facultatively methylotrophic bacteria (methylobacteria) with the ribulose monophosphate (RuMP) pathway of C 1 assimilation: Methylophaga (Janvier et al, 1985;de Zwart et al, 1996; Doronina et al, 2003a, b), Methylobacillus (Yordy & Weaver, 1977;Urakami & Komagata, 1986), Methylophilus (Jenkins et al, 1987) and Methylovorus (Govorukhina & Trotsenko, 1991).The genus Methylovorus was proposed for restricted facultative methylobacteria isolated from activated sludge, mud, soil and pond water. On the basis of some phenetic characteristics and DNA-DNA relatedness, five isolates were described as one species, Methylovorus glucosotrophus.

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