Methylomonas albis sp. nov. and Methylomonas fluvii sp. nov.: Two cold-adapted methanotrophs from the river Elbe and emended description of the species Methylovulum psychrotolerans

Bussmann, Ingeborg; Horn, Fabian; Hoppert, Michael; Klings, Karl-Walter; Saborowski, Anke; Warnstedt, Julia; Liebner, Susanne

doi:10.1016/j.syapm.2021.126248

Cited by 22 publications

(18 citation statements)

References 69 publications

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“…C16:0 is the next highest concentration of fatty acid in strain Kb3. Methylomonas fluvii, the closest phylogenetic relative to strain Kb3, expressed a higher percentage of fatty acid C14:0 (51.6%) followed by C12:0 with 26.6% (Bussmann et al 2021). M. fluvii and M.…”

Section: Chemotaxonomic Characteristics and Phylogenetic Analysismentioning

confidence: 98%

“…albis did not possess other C16 fatty acids except C16:0 (Table 2) (Bussmann et al 2021). Strain Kb3 also possessed other fatty acids like C12:0, C16:0 iso, C17:0 iso, C16:0 3OH and C18:1 ω7c / ω6c in minor percentages (Table 2).…”

Section: Chemotaxonomic Characteristics and Phylogenetic Analysismentioning

confidence: 99%

“…However, the preferred C source is methane as the aim of cultivation of these native methanotrophs is their future application for methane mitigation studies. Methylomonas albis (Bussmann et al 2021); Methylomonas methanica (Bowman et al 1993); Methylomonas koyamae (Ogiso et al 2012); Methylomonas fodinarum (Bowman et al 1990); Methylomonas scandinavica (Kalyuzhnaya et al 1999);…”

Section: Physiological Characteristicsmentioning

confidence: 99%

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Description of Methylomonas Aquatica Sp. Nov., Isolated from a Tropical Indian Rice Rhizosphere

Rahalkar¹

2022

Preprint

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An aerobic methanotroph, strain Kb3T, was isolated from a rhizospheric soil sample collected from a tropical Indian rice field. The cells were motile, Gram-negative bacilli, formed pink colonies and pink turbid/pellicles in the liquid medium. Biochemical characteristics showed that strain Kb3T utilised only methane and methanol as its sole carbon and energy sources. The isolate's 16S rRNA gene sequence expressed 99.52% similarity to the recently described valid species Methylomonas fluvii EbB (Mmf), with 92% query cover. But examining the genome similarity between Kb3 and EbB, a DDH value of 44.20% [41.6 - 46.7%] and an Ortho-ANI value of 91.48 was observed that were below the current cut-off values for species differentiation. Also, the 16S rRNA gene phylogeny and the phylogenomic analysis branched the two species separately. The major fatty acid in Methylomonas sp. Kb3 was C14:0, followed by C16:1 ω5c. The genome sequence revealed the size of strain Kb3 is 5.1 Mb, with the G + C content of 51.8%. Strain Kb3T shared the closest relatedness with Methylomonas sp. LW13 reveals a 99.66% 16S rRNA gene similarity, an Ortho-ANI value of 97.9%, a DDH value of 87.3%, and a close branching in the phylogenomic tree Kb3 and LW13 together form a new species. The genomic and phylogenetic distinction between species Mmf and strain Kb3 supports Kb3T to be described as a novel species within the genus Methylomonas, with the proposed name, Methylomonas aquatica sp. nov. (Mma) and the type strain being Kb3T (=MCC 4012, =JCM 33634, =KCTC 72521).

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Section: Chemotaxonomic Characteristics and Phylogenetic Analysismentioning

confidence: 98%

Section: Chemotaxonomic Characteristics and Phylogenetic Analysismentioning

confidence: 99%

Section: Physiological Characteristicsmentioning

confidence: 99%

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Description of Methylomonas Aquatica Sp. Nov., Isolated from a Tropical Indian Rice Rhizosphere

Rahalkar¹

2022

Preprint

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“…These species possess stacks of vesicular discs throughout the cells, which is a typical intracytoplasmic membrane arrangement found in type I methanotrophs [14]. The major fatty acids of most members of the genus Methylomonas include C 14 : 0 and C 16 : 1 ω8 c [12]. However, M. albis and M. fluvii contain low quantities (8 %) of cis -branched fatty acids [12].…”

Section: Introductionmentioning

confidence: 99%

Methylomonas defluvii sp. nov., a type I methane-oxidizing bacterium from a secondary sedimentation tank of a wastewater treatment plant

Ouyang,

Wang,

Nie

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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An aerobic methanotroph was isolated from a secondary sedimentation tank of a wastewater treatment plant and designated strain OY6T. Cells of OY6T were Gram-stain-negative, pink-pigmented, motile rods and contained an intracytoplasmic membrane structure typical of type I methanotrophs. OY6T could grow at a pH range of 4.5–7.5 (optimum pH 6.5) and at temperatures ranging from 20 °C to 37 °C (optimum 30 °C). The major cellular fatty acids were C14 : 0, C16 : 1ω7c/C16 : 1ω6c and C16 : 1ω5c; the predominant respiratory quinone was MQ-8. The genome size was 5.41 Mbp with a DNA G+C content of 51.7 mol%. OY6T represents a member of the family Methylococcaceae of the class Gammaproteobacteria and displayed 95.74–99.64 % 16S rRNA gene sequence similarity to the type strains of species of the genus Methylomonas. Whole-genome comparisons based on average nucleotide identity (ANI) and digital DNA–DNA hybridisation (dDDH) confirmed that OY6T should be classified as representing a novel species. The most closely related type strain was Methylomonas fluvii EbBT, with 16S rRNA gene sequence similarity, ANI by blast (ANIb), ANI by MUMmer (ANIm) and dDDH values of 99.64, 90.46, 91.92 and 44.5 %, respectively. OY6T possessed genes encoding both the particulate methane monooxygenase enzyme and the soluble methane monooxygenase enzyme. It grew only on methane or methanol as carbon sources. On the basis of phenotypic, genetic and phylogenetic data, strain OY6T represents a novel species within the genus Methylomonas for which the name Methylomonas defluvii sp. nov. is proposed, with strain OY6T (=GDMCC 1.4114T=KCTC 8159T=LMG 33371T) as the type strain.

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“…36 The effective publication states that the type strain was also deposited as ATCC 700164 and CIP 104720, but no documentation was supplied. 37 The effective publication states that the type strain was also deposited as DSM 19099 and MTCC 7633, but no documentation was supplied. 38 The effective publication states that the type strain was also deposited as NCIMB 14023, but no documentation was supplied.…”

mentioning

confidence: 99%

Valid publication of new names and new combinations effectively published outside the IJSEM. Validation List no. 203

Oren

2022

International Journal of Systematic and Evolutionary Microbiology

558

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Methylomonas albis sp. nov. and Methylomonas fluvii sp. nov.: Two cold-adapted methanotrophs from the river Elbe and emended description of the species Methylovulum psychrotolerans

Cited by 22 publications

References 69 publications

Description of <i>Methylomonas Aquatica</i> Sp. Nov., Isolated from a Tropical Indian Rice Rhizosphere<strong> </strong>

Description of <i>Methylomonas Aquatica</i> Sp. Nov., Isolated from a Tropical Indian Rice Rhizosphere<strong> </strong>

Methylomonas defluvii sp. nov., a type I methane-oxidizing bacterium from a secondary sedimentation tank of a wastewater treatment plant

Valid publication of new names and new combinations effectively published outside the IJSEM. Validation List no. 203

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