Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands

Dedysh, Svetlana N.; Berestovskaya, Yulia Y.; Vasylieva, Lina V.; Belova, Svetlana E.; Khmelenina, V. N.; Suzina, N. E.; Trotsenko, Yu. A.; Liesack, Werner; Zavarzin, G. A.

doi:10.1099/ijs.0.02805-0

Cited by 155 publications

(141 citation statements)

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“…The MOB community of peat bogs or acidic forest soils, for example, was restricted to members of type II MOB [39,40,43]. Besides, all known acidophilic isolates belong to the genera Methylocapsa and Methylocella, both type II MOB [9][10][11][12]. The MOB community capable of the consumption of atmospheric methane was studied in different forest and grassland soils and was found to exclude members of the type I group.…”

Section: Discussionmentioning

confidence: 99%

“…The microbial conversion of methane into carbon dioxide was estimated to account for 30% to 90% in flooded rice fields [1,2], for 13% to 38% of the methane produced in temperate and subarctic peat soils [13], and for 15% to more than 90% of the diffusive methane flux in wetlands [36,47]. The group of MOB comprises the three families Methylococcaceae, Methylocystaceae, and Beijerinckiaceae [5,[9][10][11][12]. The only exception is Crenothrix polyspora, a filamentous, sheathed microorganism recently discovered to be methanotrophic [52].…”

Section: Introductionmentioning

confidence: 99%

“…Members of the Methylococcaceae are termed type I MOB and belong to the γ-subdivision of the Proteobacteria phylum. Members of the Methylocystaceae and Beijerinckiaceae are termed type II MOB and belong to the α-subdivision of the Proteobacteria phylum [10][11][12][13][14][15][16][17][18][19][20][21]. The diversity and composition of MOB have been investigated in several environments such as freshwater sediments [8,42], landfill soils [59], rice field soils [22,24], habitats with only atmospheric methane concentrations [29,33,35], and peat bogs with very low pH values [39,40].…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Aerobic Methanotrophic Bacteria in a Permafrost Active Layer Soil of the Lena Delta, Siberia

et al. 2008

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With this study, we present first data on the diversity of aerobic methanotrophic bacteria (MOB) in an Arctic permafrost active layer soil of the Lena Delta, Siberia. Applying denaturing gradient gel electrophoresis and cloning of 16S ribosomal ribonucleic acid (rRNA) and pmoA gene fragments of active layer samples, we found a general restriction of the methanotrophic diversity to sequences closely related to the genera Methylobacter and Methylosarcina, both type I MOB. In contrast, we revealed a distinct species-level diversity. Based on phylogenetic analysis of the 16S rRNA gene, two new clusters of MOB specific for the permafrost active layer soil of this study were found. In total, 8 out of 13 operational taxonomic units detected belong to these clusters. Members of these clusters were closely related to Methylobacter psychrophilus and Methylobacter tundripaludum, both isolated from Arctic environments. A dominance of MOB closely related to M. psychrophilus and M. tundripaludum was confirmed by an additional pmoA gene analysis. We used diversity indices such as the Shannon diversity index or the Chao1 richness estimator in order to compare the MOB community near the surface and near the permafrost table. We determined a similar diversity of the MOB community in both depths and suggest that it is not influenced by the extreme physical and geochemical gradients in the active layer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of Aerobic Methanotrophic Bacteria in a Permafrost Active Layer Soil of the Lena Delta, Siberia

et al. 2008

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“…are aerobic facultative methanotrophs (Alphaproteobacteria), with three validated species, M. palustris, M. silvestris and M. tundrae, isolated from acidic soils (Dunfield et al, 2003;Dedysh et al, 2000Dedysh et al, , 2004. M. silvestris BL2, which can grow on a variety of multicarbon substrates (Dedysh et al, 2005;Theisen et al, 2005), oxidizes methane using the soluble methane monooxygenase encoded by the operon mmoXYBZDC.…”

Section: Introductionmentioning

confidence: 99%

Environmental distribution and abundance of the facultative methanotroph Methylocella

et al. 2010

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Methylocella spp. are facultative methanotrophs, which are able to grow not only on methane but also on multicarbon substrates such as acetate, pyruvate or malate. Methylocella spp. were previously thought to be restricted to acidic soils such as peatlands, in which they may have a key role in methane oxidation. There is little information on the abundance and distribution of Methylocella spp. in the environment. New primers were designed, and a real-time quantitative PCR method was developed and validated targeting Methylocella mmoX (encoding the a-subunit of the soluble methane monooxygenase) that allowed the quantification of Methylocella spp. in environmental samples. We also developed and validated specific PCR assays, which target 16S rRNA genes of known Methylocella spp. These were used to investigate the distribution of Methylocella spp. in a variety of environmental samples. It was revealed that Methylocella species are widely distributed in nature and not restricted to acidic environments.

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“…Similar to many alphaproteobacteria, strain Pf56 T contained significant amounts (26.3 %) of 11-cis-octadecenoic acid (18 : 1v7c), which is also typical of members of the families Methylocystaceae and Beijerinckiaceae. However, the major component of the fatty acid profile (39 % of the total fatty acids) in our novel isolate was 19 : 0 cyclo v8c, which is absent from methanotrophs of the Methylocystaceae (Bodelier et al, 2009) and has been detected in significant amounts (up to 13 %) in only a very few members of the Beijerinckiaceae, such as Methylocella tundrae (Dedysh et al, 2004a).…”

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Descriptions of Roseiarcus fermentans gen. nov., sp. nov., a bacteriochlorophyll a-containing fermentative bacterium related phylogenetically to alphaproteobacterial methanotrophs, and of the family Roseiarcaceae fam. nov.

Kulichevskaya

Danilova

Терешина

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Descriptions of Roseiarcus fermentans gen. nov., sp. nov., a bacteriochlorophyll a-containing fermentative bacterium related phylogenetically to alphaproteobacterial methanotrophs, and of the family Roseiarcaceae fam. nov. A light-pink-pigmented, microaerophilic bacterium was obtained from a methanotrophic consortium enriched from acidic Sphagnum peat and designated strain Pf56 T . Cells of this bacterium were Gram-negative, non-motile, thick curved rods that contained a vesicular intracytoplasmic membrane system characteristic of some purple non-sulfur alphaproteobacteria. The absorption spectrum of acetone/methanol extracts of cells grown in the light showed maxima at 363, 475, 505, 601 and 770 nm; the peaks at 363 and 770 nm are characteristic of bacteriochlorophyll a. However, in contrast to purple non-sulfur bacteria, strain Pf56 T was unable to grow phototrophically under anoxic conditions in the light. Best growth occurred on some sugars and organic acids under micro-oxic conditions by means of fermentation. The fermentation products were propionate, acetate and hydrogen. Slow chemo-organotrophic growth was also observed under fully oxic conditions. Light stimulated growth. C 1 substrates were not utilized. Strain Pf56 T grew at pH 4.0-7.0 (optimum pH 5.5-6.5) and at 15-30 6C (optimum 22-28 6C). The major cellular fatty acids were 19 : 0 cyclo v8c and 18 : 1v7c; quinones were represented by ubiquinone Q-10. The G+C content of the DNA was 70.0 mol%. Strain Pf56 displays 93.6-94.7 and 92.7-93.7 % 16S rRNA gene sequence similarity to members of the families Methylocystaceae and Beijerinckiaceae, respectively, and belongs to a large cluster of environmental sequences retrieved from various wetlands and forest soils in cultivationindependent studies. Phenotypic, genotypic and chemotaxonomic characteristics of strain Pf56 T suggest that it represents a novel genus and species of bacteriochlorophyll a-containing fermentative bacteria, for which the name Roseiarcus fermentans gen. nov., sp. nov. is proposed. Strain Pf56 T (5DSM 24875 T 5VKM B-2876 T ) is the type strain of Roseiarcus fermentans, and is also the first characterized member of a novel family within the class Alphaproteobacteria, Roseiarcaceae fam. nov.

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Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands

Cited by 155 publications

References 10 publications

Diversity of Aerobic Methanotrophic Bacteria in a Permafrost Active Layer Soil of the Lena Delta, Siberia

Diversity of Aerobic Methanotrophic Bacteria in a Permafrost Active Layer Soil of the Lena Delta, Siberia

Environmental distribution and abundance of the facultative methanotroph Methylocella

Descriptions of Roseiarcus fermentans gen. nov., sp. nov., a bacteriochlorophyll a-containing fermentative bacterium related phylogenetically to alphaproteobacterial methanotrophs, and of the family Roseiarcaceae fam. nov.

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