Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent

Abstract

Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent Department of Biological Science, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, JapanA moderately thermophilic, methanol-oxidizing bacterium (strain Gela4 T ) was isolated from methane-utilizing mixed-culture originating from marine sediment near a hydrothermal vent. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain Gela4 T was closely related to members of the genus 'Methyloligella' (94.7 % similarity) within the class Alphaproteobacteria. Strain Gela4 T was a Gram-staining-negative and aerobic organism. Cells were rod-shaped and non-motile. The temperature range for growth of strain Gela4

New Methyloceanibacter diversity from North Sea sediments includes methanotroph containing solely the soluble methane monooxygenase

“…Subsequent metagenome sequencing revealed the presence of one highly enriched bacterium in sample W04 (representing 83.10% of the total reads) and two in sample W09 (together representing 87.04% of total reads). All three genomic bins were closely related with the recently described methylotrophic Alphaproteobacterium Methyloceanibacter caenitepidi Gela4 T , obtained from marine sediment collected near a hydrothermal vent in Japan (Takeuchi et al , ). Surprisingly, no pmoA genes encoding the particulate methane monooxygenase (pMMO) were detected in the metagenomes.…”

“…M. caenitepidi Gela4 T also formed single cell cultures, similar to R‐67175. All strains formed identical colonies (round, white and convex) on solid media and displayed similar rod like shaped cell morphology, similar to M. caenitepidi Gela4 T , with Gram‐negative staining (Takeuchi et al , ).…”

“…Despite their crucial role in marine carbon cycling, only a handful of marine methanol oxidizers and even fewer methane oxidizers, the latter all belonging to the Gammaproteobacteria , have been isolated and physiologically characterized (Sieburth et al , ; Lidstrom, ; Fuse et al , ; Giovannoni et al , ; Boden et al , ; Huggett et al , ; Hirayama et al , ; Hirayama et al , ; Takeuchi et al , ; Tavormina et al , ; Jimenez‐Infante et al , ). Most methanotrophic surveys focused on high methane‐loaded systems like mud vulcanoes (Niemann et al , ), vent fields (Nercessian et al , ) and methane gas seeps (Inagaki et al , ; Yan et al , ; Håvelsrud et al , ).…”

Methylocaldum marinum sp. nov., a thermotolerant, methane-oxidizing bacterium isolated from marine sediments, and emended description of the genus Methylocaldum

“…Cloning of the 16S rRNA gene of the original methaneutilizing mixed culture had revealed the existence of three strains (strains S8 T , Gela4 T and MA2). Strains Gela4 T (Methyloceanibacter caenitepidi Gela4 T ; Takeuchi et al, 2014) and MA2 were also isolated and shown to be, respectively, a methylotroph that grows on a modified NMS medium containing 1 % methanol and solidified with agar, and a heterotroph that grows on marine agar 2216 (Difco). Therefore, the purity of strain S8 T was checked by plating the culture onto these two agar media.…”

“…Marine surface sediments from a location north to the Wakamiko Crater (water depth 161 m; 31 u 41.099 N 130 u 47.1249 E) were collected in 2001 and used as an inoculum for the enrichments. A stable methane-utilizing mixed culture was established as described previously (Takeuchi et al, 2014). Strain S8 T was isolated from the mixed culture on plates made from NMS medium (Bowman, 2006), which was modified by replacing the distilled water and sodium nitrate with artificial seawater (Kester et al, 1967) and ammonium chloride, respectively, and then solidified by mixing with an equal volume of autoclaved gellan-gum solution (final concentration 1.5 %; Tamaki et al, 2005Tamaki et al, , 2009.…”