Methylobacterium pseudosasicola sp. nov. and Methylobacterium phyllostachyos sp. nov., isolated from bamboo leaf surfaces

Abstract

Two strains of Gram-negative, methylotrophic bacteria, isolated because of their abilities to promote plant growth, were subjected to a polyphasic taxonomic study. The isolates were strictly aerobic, motile, pink-pigmented, facultatively methylotrophic, non-spore-forming rods. The chemotaxonomic characteristics of the isolates included the presence of C18 : 1ω7c as the major cellular fatty acid. The DNA G+C contents of strains BL36T and BL47T were 69.4 and 69.8 mol%, respectively. 16S rRNA gene sequence analysis of strains BL36T and BL47T placed them under the genus Methylobacterium, with the pairwise sequence similarity between them and the type strains of closely related species ranging from 97.2 to 99.0 %. On the basis of their phenotypic and phylogenetic distinctiveness and the results of DNA–DNA hybridization analysis, the isolates represent two novel species within the genus Methylobacterium , for which the names Methylobacterium pseudosasicola sp. nov. (type strain BL36T = NBRC 105203T = ICMP 17621T) and Methylobacterium phyllostachyos sp. nov. (type strain BL47T = NBRC 105206T = ICMP 17619T) are proposed.

Methylobacterium sp. 2A Is a Plant Growth-Promoting Rhizobacteria That Has the Potential to Improve Potato Crop Yield Under Adverse Conditions

“…Characteristics of Methylobacterium sp. 2A, such as colony pigmentation, cell size, growth conditions, and catalase and oxidase reactions, were compared (Table S2) with those of the most closely related species (Ito and Iizuka, 1971;Kato et al, 2008;Knief et al, 2012;Madhaiyan and Poonguzhali, 2014;Madhaiyan et al, 2007;Madhaiyan et al, 2009). As observed, though isolated from different sources, all strains were positive for catalase and oxidase reactions, had a similar pigmentation, and shared several growth conditions.…”

Assessment of genetic diversity and plant growth promoting attributes of psychrotolerant bacteria allied with wheat (Triticum aestivum) from the northern hills zone of India

“…The epiphytic bacterial strains were identified as Arthrobacter methylotrophus, Pseudomonas rhodesiae, Methylobacterium mesophilicum, Methylobacterium phyllosphaerae, Methylobacterium radiotolerans and Methylobacterium sp., in which pinkpigmented facultative methylotrophs (PPFMs) were most dominant. PPFMs are reported to influence the seed germination and growth of many crop plants (Madhaiyan and Poonguzhali 2014;Verma et al 2014). Arthrobacter methylotrophus and Pseudomonas rhodesiae were identified as epiphytic PGPB for the first time herein, from wheat growing at high altitude and low temperature environments.…”

Paenibacillus polysaccharolyticus sp. nov., a xylanolytic and cellulolytic bacteria isolated from leaves of Bamboo Phyllostachys aureosulcata

“…This resulted in identification of novel species of bacteria including Methylobacterium pseudosasae [10], Methylobacterium pseudosasicola and Methylobacterium phyllostachyos [11] and Paenibacillus methanolicus [12]. The present study describes a strain, designated BL9 T , isolated from leaves of Phyllostachys aureosulcata using ammonium mineral salts (AMS) medium [13] adapted from previously published methods [12].…”