Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis

Abstract

A novel bacterial strain, designated PMB02T, was isolated from a leaf of the tree Platanus orientalis. Colonies grown on TYG agar plates were circular, pink-pigmented and slow-growing, being 0.2–1.5 mm in diameter after 3 days growth. The cells of strain PMB02T were Gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20–30 °C, pH 6–8 and in media containing less than 1 % NaCl. The major respiratory quinone was identified as Q-10. A phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain PMB02T was related to members of the genus Methylobacterium. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium aquaticum and Methylobacterium variabile, with which it showed sequence similarities of 97.7 and 97.4 %, respectively. The values for DNA–DNA hybridization between strain PMB02T and M. aquaticum CCM 7218T and M. variabile GR3T were less than 32 %. On the basis of the phenotypic characterization, the phylogenetic analysis and the DNA–DNA relatedness data, strain PMB02T is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium platani sp. nov. is proposed. The type strain is PMB02T (=KCTC 12901T=JCM 14648T).

Cultivation-Independent Characterization of Methylobacterium Populations in the Plant Phyllosphere by Automated Ribosomal Intergenic Spacer Analysis

“…The genus Methylobacterium currently comprises 28 described species (28, 60). Several new species have been described during the last few years, including species from environments that have been analyzed for several decades, such as the plant phyllosphere (23,27,28,38,58). This suggests that the diversity of Methylobacterium is still not fully known.…”

“…They have also been found in association with humans (2), and some members of this genus have increasingly been reported to be a cause of opportunistic infections in immunocompromised patients (22). The genus Methylobacterium currently comprises 28 described species (28,60). Several new species have been described during the last few years, including species from environments that have been analyzed for several decades, such as the plant phyllosphere (23,27,28,38,58).…”

Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice

“…Gelatin, starch, glycerol tributyrate, casein and aesculin are not hydrolysed. Hydrogen sulfide is not Weon et al (2008) and Kang et al (2007). Values are percentages of total fatty acids; 2, not detected.…”

Methylobacterium haplocladii sp. nov. and Methylobacterium brachythecii sp. nov., isolated from bryophytes

“…Major cellular fatty acids are C 18 : 1 v7c, C 18 : 0 and C 16 : 0 . Tani et al, 2012b); 4, Methylobacterium organophilum JCM 2833 T (Kato et al 2005); 5, Methylobacterium marchantiae JT1 T ; 6, Methylobacterium bullatum F3.2 T (Hoppe et al, 2011); 7, Methylobacterium jeotgali S2R03-9 T (Aslam et al 2007); 8, Methylobacterium cerastii C15 T (Wellner et al, 2012); 9, Methylobacterium gossipiicola Gh-105 T (Madhaiyan et al, 2012); 10, Methylobacterium phyllosphaerae CBMB27 T (Madhaiyan et al, 2009); 11, Methylobacterium platani PMB02 T (Kang et al, 2007); and 12, Methylobacterium oxalidis 35a T (Tani et al, 2012a). +, Positive; 2, negative; (+), weakly positive; NA, data not available; V, variable reaction; *, data from this study.…”