Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice

Abstract

A pink-pigmented, facultatively methylotrophic bacterium, strain CBMB20 T , isolated from stem tissues of rice, was analysed by a polyphasic approach. Strain CBMB20T utilized 1-aminocyclopropane 1-carboxylate (ACC) as a nitrogen source and produced ACC deaminase. It was related phylogenetically to members of the genus Methylobacterium. 16S rRNA gene sequence analysis indicated that strain CBMB20 T was most closely related to Methylobacterium fujisawaense, Methylobacterium radiotolerans and Methylobacterium mesophilicum; however, DNA-DNA hybridization values were less than 70 % with the type strains of these species. The DNA G+C content of strain CBMB20 T was 70.6 mol%. The study presents a detailed phenotypic characterization of strain CBMB20 T that allows its differentiation from other Methylobacterium species. In addition, strain CBMB20 T is the only known member of the genus Methylobacterium to be described from the phyllosphere of rice. Based on the data presented, strain CBMB20T represents a novel species in the genus Methylobacterium, for which the name Methylobacterium oryzae sp. nov. is proposed, with strain CBMB20 T (=DSM 18207 T =LMG 23582 T =KACC 11585 T ) as the type strain.The genus Methylobacterium includes a group of strictly aerobic, Gram-negative, pink-pigmented, facultatively methylotrophic (PPFM) bacteria characterized by their ability to utilize single-carbon compounds like methanol and formaldehyde via the serine pathway, as well as a wide range of multicarbon growth substrates (Green, 1992). Methylobacterium is classified in the a2 subgroup of the Proteobacteria and presently consists of 22 species with validly published names

Effect of Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 Inoculation on Seed Germination and Early Growth of Maize and Sorghum-sudangrass hybrid Seedling under Different Salinity Levels

“…The culture was maintained in Tryptic soy agar (TSA) medium supplemented with 5% (∼0.85 M) NaCl (pH 7.2). For experimental studies 1.0 mL of the initially mass multiplied culture (1×10 8 cfu mL ) was transferred to 100 mL fresh TSB supplemented with 5% (0.85 M) NaCl and allowed to grow for 24 h. The other bacteria used in this study was pink-pigmented facultative methylotroph (PPFM) Methylobacterium oryzae strain CBMB20, which was isolated from stem tissues of rice and well characterized in related to PGP activities (Madhaiyan et al 2007). The culture was maintained in ammonium mineral salt (AMS) media with 0.5% sodium succinate supplemented at pH 7.2.…”

Effect of Azospirillum brasilense and Methylobacterium oryzae Inoculation on Growth of Red Pepper (Capsicum annuum L.)

“…Bacterial strains and inoculum culture Methylobacterium oryzae CBMB20 (Madhaiyan et al, 2006a(Madhaiyan et al, , 2007 and Azospirillum brasilense CW903 (Kim et al, 2005) were used in this study. The strains were cultured in ammonium mineral salts minimal broth with 0.5% methanol for 4 days (M. oryzae CBMB20), and in nitrogen free malic acid broth supplemented with 1 g NH 4 Cl per liter for 48 h (A. brasilense CW903) at 30°C under shaking.…”

“…Various bacterial species have been successfully used in inoculation trials in pot experiments under greenhouse condition (Bashan and Holguin, 1997;Masheshawari, 2011). However, commercial application of plant growth promoting microbes on a large scale is still very limited, and the main obstacle is the unpredictability and inconsistency of field results (Bashan and Holguin, 1997 mg kg Recently we isolated bacterial species of Methylobacterium and Azospirillum from rice and taro, respectively, and their plant growth promoting abilities were confirmed in laboratory and greenhouse experiments (Chauhan et al, 2010;Kim et al, 2005;Madhaiyan et al, 2004Madhaiyan et al, , 2007Madhaiyan et al, , 2010Ryu et al, 2006). Methylobacterium spp.…”

“…Thus, soil bacteria that inhabit plant roots and benefit plants by providing growth promotion are termed plant growth-promoting rhizobacteria (Bashan and Holguin 1997). Various rhizobacteria were reported to have many plant growth promoting abilities including N fixation, P solublization, production of plant growth hormones, GAs, cytokinins and auxins, and biological disease control (Madhaiyan et al, 2004(Madhaiyan et al, , 2006a(Madhaiyan et al, , 2007(Madhaiyan et al, , 2009Omer et al, 2004;Ryu et al, 2006).…”

Effect of co-inoculation of Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 on the early growth of crop plants in Saemangeum reclaimed soil

“…The genus Methylobacterium includes a group of strictly aerobic, Gram-negative, pink pigmented, facultative methylotrophic (PPFM) bacteria characterized by their ability to utilize single-carbon compounds, like methanol and formaldehyde via the serine pathway, as well as a wide range of multi-carbon growth substrates (Green, 1992). Methylobacterium oryzae CBMB20 was isolated from rice stem and has been proven to have many plant growth promoting traits (Madhaiyan et al, 2007). This bacterial strain has the ability to produce ACC deaminase, which helps in the breakdown of ACC and reduce plant ethylene level, which inhibit plant growth at high concentration (Arshad and Frankenberger, 2002).…”