Methylmercury: Some effects on embryogenesis in the Japanese Medaka, <i>Oryzias latipes</i>

Dial, Norman A.

doi:10.1002/tera.1420170116

Cited by 45 publications

(16 citation statements)

References 9 publications

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“…2), and the chance that larvae were exposed to salinities <10‰ was minimal. Pericardial edema has been described in many developmental studies with several fish species and different toxicants (Waterman 1940;Weis and Weis 1974;Dial 1978;Gillespie and Baumann 1986;Middaugh et al 1988;Marty et al 1990), but ascites is less often described. Ascites was the main lesion associated with blue-sac disease in wild lake trout (Salvelinus namaycush) larvae, and ascites also occurred in lake trout larvae exposed to 2,3,7,8-tetrachlorodibenzo-pdioxin as fertilized eggs (Symula et al 1990;Spitsbergen et al 1991).…”

Section: Level Of Significancementioning

confidence: 97%

Histopathology and cytogenetic evaluation of Pacific herring larvae exposed to petroleum hydrocarbons in the laboratory or in Prince William Sound, Alaska, after the Exxon Valdez oil spill

Marty¹,

Hose²,

McGurk³

et al. 1997

Can. J. Fish. Aquat. Sci.

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Following the 1989 Exxon Valdez oil spill in Prince William Sound, Alaska, USA, Pacific herring (Clupea pallasi) larvae sampled from oiled sites had ascites, pericardial edema, and genotoxic damage. Laboratory study confirmed that these lesions were consistent with oil exposure. Pacific herring larvae were trawled from two oiled and two unoiled sites in Prince William Sound in May 1989. Larvae from oiled sites were shorter, had ingested less food, had slower growth (oiled, 0.07-0.10 mm/day; unoiled, 0.15-0.18 mm/day), and had higher prevalence of cytogenetic damage (oiled, 56-84%; unoiled, 32-40%) and ascites (oiled, 16%; unoiled, 1%) than from unoiled sites. In the laboratory experiment, Pacific herring eggs were exposed to an oil-water dispersion of Prudhoe Bay crude oil (initial concentrations of 0.0, 0.10, 0.24, 0.48, and 2.41 mg/L) and sampled for histopathology <24 h after hatching. Effects were significant at the 0.48 mg/L dose (Dunnett's procedure, P < 0.05). Lesions included ascites; hepatocellular vacuolar change; and degeneration or necrosis of skeletal myocytes, retinal cells, and developing brain cells. Lesions in field-sampled larvae were consistent with higher mortality rates documented in larvae from oiled sites.Résumé : Après le déversement pétrolier de l'Exxon Valdez qui a eu lieu en 1989 dans le golfe du Prince-William, Alaska, É.-U., des larves de hareng du Pacifique (Clupea pallasi), prélevées dans des sites exposés au pétrole présentaient des ascites, de l'oedème péricardique et des lésions génotoxiques. Une étude en laboratoire a confirmé qu'il y avait compatibilité entre ces lésions et l'exposition au pétrole. Des larves de hareng du Pacifique ont été récoltées par chalutage dans deux sites exposés au pétrole et dans deux sites non exposés dans le golf du Prince-William en mai 1989. Les larves provenant des sites exposés étaient plus courtes, avaient ingéré moins d'aliments, avaient une croissance plus lente (site exposé, 0,07-0,10 mm/jour; site non exposé, 0,15-0,18 mm/jour) et présentaient une fréquence plus élevée de lésions cytogénétiques (site exposé, 56-84 %; site non exposé, 32-40 %) et d'ascites (site exposé, 16 %; site non exposé, 1 %) que les larves provenant des sites non exposés au pétrole. Au cours d'une expérience de laboratoire, des oeufs de hareng du Pacifique ont été exposés à une dispersion pétrole-eau de pétrole brut de Prudhoe Bay (concentrations initiales de 0,0, 0,10, 0,24, 0,48 et 2,41 mg/L) et ont été examinés pour déceler des manifestations histopathologiques moins de 24 h après leur éclosion. Les effets étaient statistiquement significatifs à la dose d'exposition de 0,48 mg/L (méthode de Dunnett, P < 0,05). Parmi les lésions figuraient des ascites, des modifications vacuolaires hépatocellulaires et une dégénérescence ou une nécrose des myocytes squelettiques, des cellules rétiniennes et des cellules cérébrales en développement. Les lésions chez les larves échantillonnées sur le terrain correspondaient avec les taux de mortalité élevés documentés chez les larves pr...

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Section: Level Of Significancementioning

confidence: 97%

Histopathology and cytogenetic evaluation of Pacific herring larvae exposed to petroleum hydrocarbons in the laboratory or in Prince William Sound, Alaska, after the Exxon Valdez oil spill

Marty¹,

Hose²,

McGurk³

et al. 1997

Can. J. Fish. Aquat. Sci.

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“…Reports on the histopathologic effects in fish are rather scarce and have been summarized by Meyers and Hendricks (16). These include studies in catfish (12,13), rainbow trout (46,47), medaka (4,5), killifish (38), and lisa (7). These studies were often focused on effects on selected organs, and pointed towards the liver, gills, and kidney as the main target organs (12,13,46,47), and towards teratogenic effects on the eye and the cardiovascular system (4,5,38).…”

Section: Introductionmentioning

confidence: 99%

Histopathological Effects in Poecilia reticulata (Guppy) Exposed to Methyl Mercury Chloride

Wester

Canton

1992

Toxicol Pathol

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To evaluate the usefulness of histopathology in aquatic toxicity testing, studies were carried out on the small freshwater fish Poecilia reticulata (guppy) following aqueous methyl mercury chloride exposure. Fish were exposed to concentrations of 0, 1.0, 1.8, 3.2, 5.6, or 10 μg/L for 1 and 3 months. Histopathological changes included the occurrence of multiple granulomas in various tissues, in particular, the integument and orbit. These changes were accompanied by hyperplasia of monocytopoietic interrenal tissue, and hepatocellular change which was confirmed by morphometry. The latter findings were probably a result of monocyte "consumption" by granulomas, and hepatic synthesis of ("stress") proteins, respectively. The bile duct and, focally, the proximal intestine, showed hyperplasia of the epithelium. In the testis of sexually mature fish (3-month study), degeneration and necrosis of sperm occurred, with severe cases exhibiting Sertoli cell hypertrophy, interstitial inflammation, and absence of mature sperm. Epidermal mucous cells disappeared in the highest concentration used, and, after 3 months, clusters of undifferentiated basophilic cells were seen in the gas gland, which occasionally were suggestive of malignant growth. The changes in the kidney tubules were characterized by degeneration and necrosis of single cells which also showed mitotic figures. This is considered a result of the mitosis-disturbing activity of methyl mercury (MeHg). It is concluded that MeHg has effects on various target organs in guppies with the occurrence of granulomas as the most sensitive indicator, yielding a no-observed-effect concentration (NOEC) of 1.0 μg/L. In contrast to mammalian species, no morphologic evidence for neurotoxicity was found.

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“…Their circulatory system, in particular, is readily visible. As a result, they have been used in toxicology for a number of years [2]. However, the study of such embryos requires a lot of measurements.…”

Section: Introductionmentioning

confidence: 99%

An Automated Assay for the Evaluation of Mortality in Fish Embryo

Puybareau

Léonard

Talbot

2015

Lecture Notes in Computer Science

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International audienceWaterways are often first and severely affected by pollution. In this context, fish embryos – which constitute a good model for sensitivity to chemicals – are widely used in environmental toxicology studies. Such studies are devoted to the analysis of a wide spectrum of physiological parameters, for instance mortality ratio. In this article, we develop an assay to determine the mortality rate of Medaka embryo. Based on video sequences, our purpose is to obtain reliable, repeatable results in a fully automated fashion. To reach that challenging goal, we develop an efficient morphological pipeline that analyses image sequences in a multiscale paradigm, from the global scene to the embryo, and then to its heart, finally analysing its putative motion, characterized by intensity variations. Our pipeline, based on robust morphological operators, has a low computational cost, and was experimentally assessed on a dataset consisting of 660 images, providing a success ratio higher than 99%

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Methylmercury: Some effects on embryogenesis in the Japanese Medaka, Oryzias latipes

Cited by 45 publications

References 9 publications

Histopathology and cytogenetic evaluation of Pacific herring larvae exposed to petroleum hydrocarbons in the laboratory or in Prince William Sound, Alaska, after the Exxon Valdez oil spill

Histopathology and cytogenetic evaluation of Pacific herring larvae exposed to petroleum hydrocarbons in the laboratory or in Prince William Sound, Alaska, after the Exxon Valdez oil spill

Histopathological Effects in Poecilia reticulata (Guppy) Exposed to Methyl Mercury Chloride

An Automated Assay for the Evaluation of Mortality in Fish Embryo

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