Methylisothiazolinone: Dermal and respiratory immune responses in mice

Cross‐reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay

“…and Devos et al . found that MI has strong sensitizing capabilities . In this study we used a verified modification of the local lymph node assay, where the sensitization phase lasted for three consecutive days.…”

“…In accordance with our results, studies by Basketter et al and Devos et al found that MI has strong sensitizing capabilities. 18,23 In this study we used a verified modification of the local lymph node assay, where the sensitization phase lasted for three consecutive days. Our data show that the mice were sensitized to MI, and, as expected, that 0Á4% MI (the previously published EC3 value for MI) induced a threefold increase of cells in the draining lymph node (data not shown).…”

Effects of chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT) on Th2/Th17-related immune modulation in an atopic dermatitis mouse model

“…Our present study findings have demonstrated that CMIT/MIT epicutaneous exposure increase Th2-related responses. Likewise, a prior experimental study reported that the epicutaneous exposure to MIT induced an increase in T cell proliferation in mice 18 . Hence, it may be possible that exposure to CMIT/MIT in the mouse induces and enhances allergic AD-like consequences, mediated via Th2-related responses.…”

“…In addition, CMIT/MIT exposure induces a systemic allergic reaction and decrease in lung function, resulting in occupational asthma [15][16][17] . A recent experimental study in mice has provided evidence of a biological basis for MIT as a risk factor for allergic sensitization, as indicated by enhanced skin inflammation, immunoglobulin E (IgE) production and immune responsiveness 18 . Exposure to CMIT/MIT has the potential to enhance the sensitization to allergens, and therefore may play a crucial role in the development of allergic diseases.…”

Airborne allergic contact dermatitis caused by isothiazolinones in water‐based paints: a retrospective study of 44 cases

“…Besides cutaneous lesions, 22.7% of our patients also reported mucosal symptoms, such as dyspnoea, rhinitis, cough, and/or conjunctivitis, indicating possible respiratory allergy, as has been suggested (3-5). Recently, however, an experimental study investigating the capacity of MI to induce asthma-like responses in a validated mouse model concluded that MI was not capable of producing asthma symptoms (16). Further research is needed to better describe the potential respiratory effects of MI, and of other isothiazolinones, in humans.…”