Methylenetetrahydrofolate Reductase C677T polymorphism and breast cancer risk in Moroccan women

Abstract

Background: Methylenetetrahydrofolate (MTHFR) enzyme plays an important role in folate metabolism which is involved in DNA methylation, repair, and synthesis. Objective: We investigated if the MTHFR C677T polymorphism modulates the risk of developing breast cancer in Moroccan women. Methods: Genotyping was performed by PCR-RFLP method on a sample of 96 patients with breast cancer and 117 controls. Results: A positive correlation was found between the MTHFR C677T polymorphism and progesterone receptors expression (p= 0.04). According to menopausal status, the heterozygous CT (OR = 2.29 and P = 0.03) was statistically significant in pre-menopausal women. There was a significant association between C677T polymorphism and breast cancer risk in both additive (OR = 2.2, 95% CI = 1.24-3.86, p = 0.007) and dominant (OR = 2.10, CI 95% = 1.21-3.64, p = 0.008) models. In addition, the T allele were associated with a high breast cancer risk (OR = 1.59, 95% CI = 1.04-2.44, p = 0.03). Conclusion:In the light of our preliminary study, 677T allele and 677CT MTHFR genotype may represent a genetic determinant increasing breast cancer risk in Moroccan women. A larger study including a larger sample size and more information is needed to confirm our conclusions.

Association between dietary intake of folate and MTHFR and MTR genotype with risk of breast cancer

“…However, we observed no significant association of intake of folate, vitamin B6 and vitamin B12 with risk of breast cancer, and no significant interaction between MTHFR C677T polymorphism and folate intake on the risk of breast cancer. Recently, several studies have reported the association between MTHFR C677T and A1298C and MTR A2756G polymorphisms and risk of breast cancer, but the results of our study are consistent with those of previous studies (Cho et al, 2007;Larsson et al, 2007;Gao et al, 2009;Webb et al, 2011;de Cássia Carvalho Barbosa et al, 2012a, b;Lajin et al, 2012;Diakite et al, 2012). Therefore, we investigated the role of MTHFR C677T and A1298C and MTR A2756G polymorphisms in the risk of breast cancer.…”

Association of dietary intake of folate and MTHFR genotype with breast cancer risk

“…We found no association between MTHFR A1298C polymorphism and breast cancer risk. Our findings are consistent with previous studies on the association between variants of MTHFR gene and folate and risk of breast cancer (Gao et al, 2009;Alshatwi, 2010;Hosseini et al, 2011;de Cássia Carvalho et al, 2012;Diakite et al, 2012;Lajin et al, 2012;Wu et al, 2012).…”

Role of MTHFR C677T and MTR A2756G polymorphisms in thyroid and breast cancer development

“…Another study in a Moroccan population conducted by Diakite et al (2012) involving 96 women found an association between at least one polymorphic allele and breast cancer increased risk, contrary to our findings. In our study, we found no statistically significant differences between 677CT+TT and 677CT genotypes in both types of cancers studied.…”

“…This polymorphism is associated with a reduction in the MTR enzyme, leading to elevated homocysteine level and DNA hypomethylation (Hosseini, 2013). Studies on polymorphisms and cancer risk presented controversial results due to several factors such as a measurement sample, ethnicity and population studied, hormones, and environmental factors such as folate intake (Diakite et al, 2012;Gong et al, 2015).…”

“…Sample calculation was performed according the reports of Ozdemir et al (2012) and Diakite et al (2012). This study is the first to evaluate polymorphisms in folate metabolism with regard to thyroid cancer development in the Brazilian population.…”