Methylene chloride fraction of Scutellaria barbata induces apoptosis in human U937 leukemia cells via the mitochondrial signaling pathway

Cha, Yun-Yi; Lee, Eun-Ok; Lee, Hyo‐Jeong; Park, Young-Doo; Ko, Seong‐Gyu; Kim, Dong‐Hee; Kim, Hyung-Min; Kang, In‐Cheol; Kim, Sung Hoon

doi:10.1016/j.cccn.2004.04.013

Cited by 54 publications

(46 citation statements)

References 23 publications

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“…For this reason, Bcl-2 proteins are an attractive target for the development of novel anticancer drugs (Chang et al, 2005). In numerous studies it was determined that alterations in Bcl-2/Bax ratio were caused by downregulation of Bcl-2, Bax (Cha et al, 2004;Paris et al, 2007;Mohammad et al, 2008) and downregulation of Bcl-2 with no change in the level of Bax (Han et al, 2008). In our study we determined no Bcl-2 expression of extract treated cells, Bax-α expression level was also decreased but still ongoing.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells

Çakır¹,

Pekmez²,

Cepni³

et al. 2014

Food Sci. Technol (Campinas)

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Section: Resultsmentioning

confidence: 99%

Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells

Çakır¹,

Pekmez²,

Cepni³

et al. 2014

Food Sci. Technol (Campinas)

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“…Naturally occurring dietary supplements and herbs have stimulated great interest because of their chemopreventive and chemotherapeutic properties as well as their relatively non-toxic effects, low cost and availability as oral formulations (41)(42)(43)(44). Scutellaria barbata used in Chinese medicine has been shown to provide protection against several major liver, lung, and rectal cancers (17,45). In this regard, we focused our studies on the effects of SBE extracts on cell proliferation in a human promyelocytic leukemia cell line with the objective of elucidating the signaling mechanism through which they exert their effects.…”

Section: Discussionmentioning

confidence: 99%

“…Scutellaria barbata has been used as a remedy for various cancers, inflammation and urinary disease in Chinese medicine. Recent studies have demonstrated that Scutellaria barbata extract (SBE) has antiproliferative properties towards U937 leukemia cells via the mitochondrial signaling pathway (17). The present study was carried out to investigate the mechanisms by which SBE inhibits the growth of human myeloid leukemia HL-60 cells at a biochemical level.…”

Section: Introductionmentioning

confidence: 99%

Induction of G1 arrest and apoptosis by Scutellaria barbata in the human promyelocytic leukemia HL-60 cell line

Kim

Kwon²,

Han³

et al. 2007

Int J Mol Med

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Abstract. Scutellaria barbata has been used to treat cancer in Chinese medicine. The responsible anticancer mechanism, however, is not clear. Here we demonstrated an inhibitory mechanism due to a Scutellaria barbata extract (SBE) on a human promyelocytic leukemia cell line (HL-60) that has a mutation in the tumor suppressor gene p53. HL-60 cells were incubated with various concentrations of SBE. After a 24-h incubation, cytotoxicity and apoptosis were determined by MTT and DNA fragmentation assay, respectively. After treatment with SBE, cell cycle arrest was determined by measuring the cell number stained by 5'-bromo-2'-deoxyuridine (BrdU) and 7-amino-actinomycin D (7-AAD). Treatment of cells with SBE resulted in a concentration-and timedependent inhibition of growth and a G 1 phase arrest of the cell cycle. This effect was associated with a marked decrease in the protein expression of cyclin A, D1, D2, D3, and E and their activating partners, cyclin-dependent kinases (CDK) 2, 4, and 6 with concomitant upregulation of p21, cyclindependent kinase inhibitor. Downstream of the CDK inhibitory protein-CDK/cyclin cascade, SBE decreased phosphorylation level of retinoblastoma protein. SBE treatment also resulted in apoptosis evidenced by an increase of sub-G 1 phase cells, DNA fragmentation and degradation of the inhibitory protein for the caspase-activated deoxyribonuclease. The molecular mechanism during SBE-mediated growth inhibition in HL-60 cells may be due to modulation of the cell-cycle machinery and the induction of apoptosis.

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“…This herbal agent is known to contain a large number of alkaloids and flavones, frequently found as glucoside and other constituents, including phenethyl alcohols, sterols, essential oils and amino acids. It was found that a 30% ethanol extract of S. barbata induced apoptosis in the lung cancer A549 cell line (28), the methylene chloride fraction of S. barbata induced apoptosis in human U937 leukemia cells via the mitochondrial signaling pathway (29), and the chloroform fraction exhibited strong cytotoxicity on the cancer cell line Bel-7402 with a lower cytotoxic effect on a normal liver cell line (30). It has been suggested that the extracts of S. barbata exert their antitumor and antiproliferative effects through different mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Antitumor and anti-angiogenic activities of Scutellaria barbata extracts in vitro are partially mediated by inhibition of Akt/protein kinase B

et al. 2011

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Abstract. The Akt pathway is considered a pivotal player in regulating cell survival, growth, migration and angiogenesis. Disruption of normal Akt/PKB/PTEN signaling frequently occurs in numerous types of human cancers. Therefore, this signaling pathway is regarded as an important target for effective cancer therapeutic strategies. In the present study, methanol extracts from Scutellaria barbata (S. barbata) were determined to be Akt/protein kinase B inhibitory, after screening a panel of 40 traditional Chinese herbs with the Fast Activated Cellbased ELISA (FACE) assay. S. barbata extracts were found to suppress the phosphorylation levels of Akt. This inhibition was Akt kinase-specific as it had no effect on PI3K, the upstream kinase of Akt, whereas the levels of phosphorylated Bad and FHKR, the two downstream targets of Akt, changed as the levels of Akt changed. S. barbata extracts also exhibited cytotoxicity against LoVo and human umbilical vein endothelial cells (HUVECs). Furthermore, this extract inhibited the process of in vitro angiogenesis of HUVECs on Matrigel. S. barbata may be a suitable alternative source with which to isolate small molecules for use as Akt kinase inhibitors.

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Methylene chloride fraction of Scutellaria barbata induces apoptosis in human U937 leukemia cells via the mitochondrial signaling pathway

Cited by 54 publications

References 23 publications

Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells

Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells

Induction of G1 arrest and apoptosis by Scutellaria barbata in the human promyelocytic leukemia HL-60 cell line

Antitumor and anti-angiogenic activities of Scutellaria barbata extracts in vitro are partially mediated by inhibition of Akt/protein kinase B

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