Methylene blue prevents retinal damage in an experimental model of ischemic proliferative retinopathy

Rey-Funes, Manuel; Larráyoz, Ignacio M.; Fernández, Juan Carlos; Contartese, Daniela S; Rolón, Federico; Inserra, Pablo Ignacio Felipe; Martı́nez-Murillo, Ricardo; López‐Costa, Juan José; Dorfman, Verónica Berta; Martı́nez, Alfredo; Loidl, César Fabián

doi:10.1152/ajpregu.00266.2015

Cited by 16 publications

(10 citation statements)

References 62 publications

(73 reference statements)

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“…Sections were stained with hematoxylin and eosine. The number of RGCs per 25x objective microscopic field was estimated in each experimental group, as previously reported 54 . Additional retinas, taken from animals sacrificed 6 days after surgery, were stained for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) with the In Situ Cell Death Detection Kit, POD (Roche, Basel, Switzerland), following manufacturer´s instructions.…”

Section: Methodsmentioning

confidence: 99%

Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat

Rey-Funes

Larráyoz

Contartese

et al. 2017

Sci Rep

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Ocular and periocular traumatisms may result in loss of vision. Hypothermia provides a beneficial intervention for brain and heart conditions and, here, we study whether hypothermia can prevent retinal damage caused by traumatic neuropathy. Intraorbital optic nerve crush (IONC) or sham manipulation was applied to male rats. Some animals were subjected to hypothermia (8 °C) for 3 h following surgery. Thirty days later, animals were subjected to electroretinography and behavioral tests. IONC treatment resulted in amplitude reduction of the b-wave and oscillatory potentials of the electroretinogram, whereas the hypothermic treatment significantly (p < 0.05) reversed this process. Using a descending method of limits in a two-choice visual task apparatus, we demonstrated that hypothermia significantly (p < 0.001) preserved visual acuity. Furthermore, IONC-treated rats had a lower (p < 0.0001) number of retinal ganglion cells and a higher (p < 0.0001) number of TUNEL-positive cells than sham-operated controls. These numbers were significantly (p < 0.0001) corrected by hypothermic treatment. There was a significant (p < 0.001) increase of RNA-binding motif protein 3 (RBM3) and of BCL2 (p < 0.01) mRNA expression in the eyes exposed to hypothermia. In conclusion, hypothermia constitutes an efficacious treatment for traumatic vision-impairing conditions, and the cold-shock protein pathway may be involved in mediating the beneficial effects shown in the retina.

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Section: Methodsmentioning

confidence: 99%

Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat

Rey-Funes

Larráyoz

Contartese

et al. 2017

Sci Rep

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“…For instance, it has been widely reported that it is a relatively potent inhibitor of SCD (IC 50 = 0.9 uM). However, SA is also able to mediate anti-inflammatory and protective effects in the retina independently of SCD (AP, RP, APS, IML, in preparation) by a mechanism that could also involve the toll-like receptor 4 (TLR4) receptor and intracellular kinases [58] and could be of interest in other retinal pathologies [120,121].…”

Section: Sa and Therapeutic Opportunitiesmentioning

confidence: 99%

Sterculic Acid: The Mechanisms of Action beyond Stearoyl-CoA Desaturase Inhibition and Therapeutic Opportunities in Human Diseases

Peláez

Pariente

Pérez-Sala

et al. 2020

Cells

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In many tissues, stearoyl-CoA desaturase 1 (SCD1) catalyzes the biosynthesis of monounsaturated fatty acids (MUFAS), (i.e., palmitoleate and oleate) from their saturated fatty acid (SFA) precursors (i.e., palmitate and stearate), influencing cellular membrane physiology and signaling, leading to broad effects on human physiology. In addition to its predominant role in lipid metabolism and body weight control, SCD1 has emerged recently as a potential new target for the treatment for various diseases, such as nonalcoholic steatohepatitis, Alzheimer’s disease, cancer, and skin disorders. Sterculic acid (SA) is a cyclopropene fatty acid originally found in the seeds of the plant Sterculia foetida with numerous biological activities. On the one hand, its ability to inhibit stearoyl-CoA desaturase (SCD) allows its use as a coadjuvant of several pathologies where this enzyme has been associated. On the other hand, additional effects independently of its SCD inhibitory properties, involve anti-inflammatory and protective roles in retinal diseases such as age-related macular degeneration (AMD). This review aims to summarize the mechanisms by which SA exerts its actions and to highlight the emerging areas where this natural compound may be of help for the development of new therapies for human diseases.

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“…The changes observed in the retina of asphyctic animals include ganglion cell death, neovascularization, and Müller cell hypertrophy in the inner retina (Rey-Funes et al, 2013). The nitrergic system is one of the factors involved in the genesis of all these lesions (Rey-Funes et al, 2011;Rey-Funes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

et al. 2021

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Perinatal asphyxia (PA) can cause retinopathy and different degrees of visual loss, including total blindness. In a rat model of PA, we have previously shown a protective effect of hypothermia on the retina when applied simultaneously with the hypoxic insult. In the present work, we evaluated the possible protective effect of hypothermia on the retina of PA rats when applied immediately after delivery. Four experimental groups were studied: Rats born naturally as controls (CTL), animals that were exposed to PA for 20 min at 37°C (PA), animals exposed to PA for 20 min at 15°C (HYP), and animals that were exposed to PA for 20 min at 37°C and, immediately after birth, kept for 15 min at 8°C (HYP-PA). To evaluate the integrity of the visual pathway, animals were subjected to electroretinography at 45 days of age. Molecular (real time PCR) and histological (immunohistochemistry, immunofluorescence, TUNEL assay) techniques were applied to the eyes of all experimental groups collected at 6, 12, 24, and 48 h, and 6 days after birth. PA resulted in a significant reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram. All animals treated with hypothermia had a significant correction of the a-wave and OP, but the b-wave was fully corrected in the HYP group but only partially in the HYP-PA group. The number of TUNEL-positive cells increased sharply in the ganglion cell layer of the PA animals and this increase was significantly prevented by both hypothermia treatments. Expression of the cold-shock proteins, cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3), was undetectable in retinas of the CTL and PA groups, but they were highly expressed in ganglion neurons and cells of the inner nuclear layer of the HYP and HYP-PA groups. In conclusion, our results suggest that a post-partum hypothermic shock could represent a useful and affordable method to prevent asphyxia-related vision disabling sequelae.

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Methylene blue prevents retinal damage in an experimental model of ischemic proliferative retinopathy

Cited by 16 publications

References 62 publications

Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat

Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat

Sterculic Acid: The Mechanisms of Action beyond Stearoyl-CoA Desaturase Inhibition and Therapeutic Opportunities in Human Diseases

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

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