Methylene Blue Prevents Pulmonary Injury after Intestinal Ischemia-Reperfusion

Galili, Yair; Ben‐Abraham, Ron; Weinbroum, A; Marmur, Silvia; Iaina, Adrian; Volman, Yoram; Peer, G; Szold, Oded; Soffer, Dror; Klausner, Josef; Rabau, Micha; Kluger, Yoram

doi:10.1097/00005373-199808000-00004

Cited by 41 publications

(43 citation statements)

References 17 publications

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“…In addition, MB has been shown effective against oxidative stress (12,13). It is also known that hepatic devascularization increases oxidative stress and lipid peroxidation parameters (14,15).…”

Section: Introductionmentioning

confidence: 99%

Methylene blue as an antioxidant agent in experimentally-induced injury in rat liver

Bozkurt¹,

Dumlu²,

Tokaç³

et al. 2015

BLL

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Background:We aimed to test the methylene blue (MB) as a dye and also to test its antioxidant activities in devascularization-induced liver injury. Methods: Twenty rats weighing 240-280 g were randomly divided into two groups, each containing 10 rats. High-grade liver injury was induced by using a pair of long pliers with blades. MB was injected into portal vein of the rats with no hepatic injury (Group 1; control group) and those with injured livers (Group 2; injury group). Liver and hepatic function tests, paraoxonase, stimulated paraoxonase, arylesterase activity, total antioxidant, and oxidant status were evaluated before and 24 h after MB injection. Results: MB did not stain the non-perfused area. Total antioxidant status decreased signifi cantly in Group 2 at hour 24 compared to Group 1. In Group 2, total antioxidant status was lower at hour 24 compared to hour 0. Total oxidant status in Group 2 at hour 0 increased signifi cantly compared to Group 1. Total oxidant status in Group 2 at hour 24 was lower compared to that at hour 0. Lipid peroxidation parameters did not alter due to devascularization. Conclusion: MB is useful in defi ning the devascularization area. Moreover, it showed to have a benefi cial effect on oxidant status (Tab. 3, Fig. 3, Ref. 25). Text in PDF www.elis.sk.

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Section: Introductionmentioning

confidence: 99%

Methylene blue as an antioxidant agent in experimentally-induced injury in rat liver

Bozkurt¹,

Dumlu²,

Tokaç³

et al. 2015

BLL

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“…During reperfusion, however, the accumulated hypoxanthine is transformed by the xanthine oxidase, in the presence of molecular oxygen, into free radicals of oxygen: superoxides, peroxides, and hydroxysl 6,7,8 , resulting in a systemic inflammatory process characterized by the increase in endothelial permeability to fluids, macromolecules, and inflammatory cells 6,9,10 .The deleterious effects of reperfusion surpass the effects of the actual ischemia, since the metabolic toxins accumulated during the period of ischemia will be distributed throughout other organs 1,11,12,13 . In the intestine, IR results in tissue lesion and morphological alterations, compromising the absorptive function 14 .…”

Section: Introductionmentioning

confidence: 99%

The protective effect of methylene blue in lungs, small bowel and kidney after intestinal ischemia and reperfusion

et al. 2008

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PURPOSE: To study the role of methylene blue as an inhibitor of superoxide production by xanthine oxidase. METHODS: Thirty-two Wistar rats were divided into 2 groups of 16 animals: the control group and the experimental group. All were submitted to a laparotomy for the occlusion of the cranial mesenteric artery during 60 minutes. The reperfusion was confirmed by the pulsation of the artery after the release of the temporary ligature and color change of the intestines. In the animals of the control group, 2 ml of saline were injected in the peritoneal cavity and in the animals of the experimental group, 2 ml of methylene blue were injected in the peritoneal cavity. After reperfusion for 4 hours, the animals were then sacrificed. The lungs were excised from all 32 rats. Simultaneously, the small intestine and kidneys were ressected in 20 animals (10 from the control group and 10 from the experimental group). Samples of the organs were taken to evaluate the action of xanthine-oxidase, for histopathology studies and for characterization of the edema. RESULTS: In the animals of the experimental group, the inflammatory lesion as well as the edema in the lung was greater than in the control group. The intestinal and renal lesions were similar in both groups, but the lung damage was superior to that observed in the intestines and kidneys. . CONCLUSION: Despite similar action of the xanthine oxidase in the control and the experimental group, after intestinal ischemia and reperfusion, the protective effect of methylene blue was observed only in the lungs of the experimental group.

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“…For the same reason this molecule was chosen in other experiments [23][24][25][26] and in the present study. On the other side, the study of Miranda et al 27 demonstrated that MB infusion had not beneficial effect on the healing of intestinal anastomoses in an experimental I/R model in rats.…”

Section: Discussionmentioning

confidence: 99%

Direct intraperitoneal resuscitation with lidocaine, methylene blue and pentoxiphylline combination does not decreases inflammation after intestinal ischemia-reperfusion injury in rats

et al. 2016

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PURPOSE:To evaluate the effects of an intraperitoneal solution of methylene blue (MB), lidocaine and pentoxyphylline (PTX) on intestinal ischemic and reperfusion injury METHODS: Superior mesenteric artery was isolated and clamped in 36 adult male Sprague Dawley rats. After 60 minutes, clamp was removed and a group received intraperitoneally UNITO solution (PTX 25mg/kg + lidocaine 5mg/kg + MB 2mg/kg), while the other group was treated with warm 0.9% NaCl solution. Rats were euthanized 45 min after drug administration. Lung and bowel were collected for histological evaluation (using Park's score) and determination of myeloperoxidase (MPO) and malondialdehyde (MDA) levels. RESULTS:Control samples showed lymphoplasmocytic infiltrate and crypt necrosis of villi. MPO and MDA measurements shown no differences between treated and control groups. CONCLUSION:The combination of lidocaine, methylene blue and pentoxyphylline administered intraperitoneally at the studied dose, did not decreased histological lesion scores and biochemical markers levels in intestinal ischemia/reperfusion injury.

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Methylene Blue Prevents Pulmonary Injury after Intestinal Ischemia-Reperfusion

Abstract: Methylene blue attenuated lung injury after intestinal ischemia-reperfusion. Inhibition of oxygen free radicals may be the protective mechanism.

Cited by 41 publications

References 17 publications

Methylene blue as an antioxidant agent in experimentally-induced injury in rat liver

Methylene blue as an antioxidant agent in experimentally-induced injury in rat liver

The protective effect of methylene blue in lungs, small bowel and kidney after intestinal ischemia and reperfusion

Direct intraperitoneal resuscitation with lidocaine, methylene blue and pentoxiphylline combination does not decreases inflammation after intestinal ischemia-reperfusion injury in rats

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