Methylene blue photoinactivation of RNA viruses

Floyd, Robert A.; Schneider, Jens; Dittmer, Dirk P.

doi:10.1016/j.antiviral.2003.11.004

Cited by 108 publications

(102 citation statements)

References 42 publications

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“…Viral structures, such as the envelope or the capsid, can be targets of photodynamic inactivation leading to loss of infectivity (Müller-Breitkreutz et al, 1995) by inhibiting the adhesion of the virus to the host cell and its penetration (O'Brien et al, 1992; or impeding viral replication by inducing damages into viral nucleic acids (Specht, 1994). Due to the short half-life and high reactivity of ROS, especially of singlet oxygen, the main targets of photodynamic inactivation are the microbial external structures (Ben-Hur et al, 1992;Malik et al, 1993;Schneider et al, 1998;Smetana et al, 1998;Gábor et al, 2001;Egyeki et al, 2003;Floyd et al, 2004;Hotze et al, 2009). Enveloped viruses are significantly more sensitive to photosensitization than their non-enveloped counterparts, due to the decomposition of envelope lipids and proteins (Costa et al, 2013).…”

Section: Introductionmentioning

confidence: 98%

SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer

Costa

Esteves

Correia

et al. 2014

Journal of Virological Methods

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Section: Introductionmentioning

confidence: 98%

SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer

Costa

Esteves

Correia

et al. 2014

Journal of Virological Methods

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“…For example in the inactivation of HIV-1 in blood products [4,5]. MB + is also a suitable photodynamic antimicrobial agent for blood and blood products [1].…”

Section: Introductionmentioning

confidence: 99%

Kinetics of the methylene blue oxidation by cerium(IV) in sulphuric acid solutions

Katafias

Kita

Wrzeszcz

et al. 2006

Transition Met Chem

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The oxidation of methylene blue (MB + ) by cerium(IV) was studied in 0.1-5 M H 2 SO 4 . The reaction proceeds via MB radical (MB 2+• ) formed by one electron transfer to the oxidant. The radical is observed spectrophotometrically by a very intense absorbance at k max = 526 nm and by the e.p.r signal at g = 2.000. The kinetics of the fast radical formation are two orders of magnitude slower than its decomposition, which were examined using a stopped-flow method at 298 K under pseudo-first order conditions. The rate laws for the both steps were determined and a likely mechanism reported.

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“…Plasma from horse A2150 was collected aseptically and stored at Ϫ20°C. Before infusion into SCID foals, the plasma was treated with 1 M methylene blue and white light (methylene blue-white light) as described previously (15,40) to inactivate potential infectious virus. Briefly, plasma was thawed, and methylene blue was added, followed by exposure to white light at 2,800 lumens for 1 h. The plasma was then refrozen at Ϫ20°C until needed for infusion.…”

Section: Methodsmentioning

confidence: 99%

Selection of a Rare Neutralization-Resistant Variant following Passive Transfer of Convalescent Immune Plasma in Equine Infectious Anemia Virus-Challenged SCID Horses

Taylor

Leib

Carpenter

et al. 2010

J Virol

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Vaccines preventing HIV-1 infection will likely elicit antibodies that neutralize diverse strains. However, the capacity for lentiviruses to escape broadly neutralizing antibodies (NAbs) is not completely understood, nor is it known whether NAbs alone can control heterologous infection. Here, we determined that convalescent immune plasma from a horse persistently infected with equine infectious anemia virus (EIAV) neutralized homologous virus and several envelope variants containing heterologous principal neutralizing domains (PND). Plasma was infused into young horses (foals) affected with severe combined immunodeficiency (SCID), followed by challenge with a homologous EIAV stock. Treated SCID foals were protected against clinical disease, with complete prevention of infection occurring in one foal. In three SCID foals, a novel neutralizationresistant variant arose that was found to preexist at a low frequency in the challenge inoculum. In contrast, SCID foals infused with nonimmune plasma developed acute disease associated with high levels of the predominant challenge virus. Following transfer to an immunocompetent horse, the neutralization-resistant variant induced a single febrile episode and was subsequently controlled in the absence of type-specific NAb. Long-term control was associated with the presence of cytotoxic T lymphocytes (CTL). Our results demonstrate that immune plasma with neutralizing activity against heterologous PND variants can prevent lentivirus infection and clinical disease in the complete absence of T cells. Importantly, however, rare neutralizationresistant envelope variants can replicate in vivo under relatively broad selection pressure, highlighting the need for protective lentivirus vaccines to elicit NAb responses with increased breadth and potency and/or CTL that target conserved epitopes.

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Methylene blue photoinactivation of RNA viruses

Cited by 108 publications

References 42 publications

SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer

SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer

Kinetics of the methylene blue oxidation by cerium(IV) in sulphuric acid solutions

Selection of a Rare Neutralization-Resistant Variant following Passive Transfer of Convalescent Immune Plasma in Equine Infectious Anemia Virus-Challenged SCID Horses

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