Methylene blue internalization and photodynamic action against clinical and ATCC Pseudomonas aeruginosa and Staphyloccocus aureus strains

Pereira, André Henrique Correia; Pinto, Juliana Guerra; Freitas, Mirian Aparecida Alves; Fontana, Letícia Corrêa; Soares, Cristina Pacheco; Ferreira‐Strixino, Juliana

doi:10.1016/j.pdpdt.2018.02.008

Cited by 22 publications

(13 citation statements)

References 24 publications

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“…The results showed that methylene blue was internalized in the S. aureus strains. When evaluating bacterial inactivation after the application of aPDT with a fluence of 25 J/cm 2 , the reduction was of approximately 4 logs in relation to the control, not resulting in complete inactivation, as observed in this study using the PDZ as PS [11]. Such results demonstrate that, despite internalization, the type of compound used as PS may directly affect the success of aPDT, not depending only on the internalization of this PS into the bacterial cells to cause inactivation of the strains.…”

Section: Discussionsupporting

confidence: 67%

“…The suspension was again centrifuged, the PDZ was removed, and the bacteria fixed with 4% paraformaldehyde and adhered to coverslips previously treated with Poli-Llysine. After 24 h, the slides were mounted and analyzed under an LSM 700 Zeiss Confocal Microscope with excitation for DAPI (4 ,6-Diamidino-2-Phenylindole, Dihydrochloride) at 405 nm and PDZ at 555 nm [11].…”

Section: Internalization Of Photodithazine ® In the Mssa And Mrsa Strains With Confocal Microscopymentioning

confidence: 99%

“…The aPDT employs a photosensitizer (PS) that is excited by the light emitted at a particular wavelength by a light source, after being internalized into the cell. In this process, it interacts with molecular oxygen producing highly toxic Reactive Oxygen Species (ROS) that result in bacterial inactivation [9][10][11]. In this sense, aPDT has been suggested as an efficient alternative approach to be applied to the management of clinical contact surfaces, disinfection of surgical instruments, biofouling, and even antimicrobial treatment of wastewater [12].…”

Section: Introductionmentioning

confidence: 99%

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Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains

et al. 2021

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Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.

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Section: Discussionsupporting

confidence: 67%

Section: Internalization Of Photodithazine ® In the Mssa And Mrsa Strains With Confocal Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains

et al. 2021

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“…Previous aPDT studies already showed that MB was an efficient PS in the inactivation of various microorganisms, namely other Gram-positive bacteria such as Staphylococcus aureus, Gram-negative bacteria such as Pseudomonas aeruginosa [32] and Vibrio parahaemolyticus [33], and fungi such as Colletotricum abscissum [34] and Candida albicans [35].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Photodynamic Therapy in the Control of Pseudomonas syringae pv. actinidiae Transmission by Kiwifruit Pollen

et al. 2020

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Pseudomonas syringae pv. actinidiae (Psa) is a phytopathogen responsible for bacterial canker in kiwifruit plants and can be disseminated through pollen. This study aimed to evaluate the effectiveness of antimicrobial photodynamic therapy (aPDT) in the inactivation of Psa on kiwifruit pollen using New Methylene Blue (NMB) and Methylene Blue (MB) in the presence/absence of potassium iodide (KI). Pollen germination assays were also performed to evaluate if it was affected by aPDT. Higher reduction of Psa was achieved using NMB (5.0 μM) combined with KI (100 mM) in vitro (ca. 8 log CFU mL−1 after 90 min of irradiation), while NMB alone promoted a lower reduction (3.7 log CFU mL−1). The most efficient NMB concentration with KI was used to study the photodynamic efficiency of MB (5.0 μM). MB with KI photo-inactivated Psa more efficiently than NMB, causing the same bacterial reduction (ca. 8 log CFU mL−1) in half the irradiation time (45 min). Therefore, MB was selected for the subsequent ex vivo aPDT assays in pollen. Almost all the Psa cells added artificially to the pollen (3.2 log CFU mL−1) were photo-inactivated (3.1 log CFU mL−1), whereas aPDT had a low effect on pollen natural microorganisms. When KI was added, a significant increase in aPDT effectiveness was observed (4.5 log CFU mL−1). No negative effects were observed in the pollen germination after aPDT. The results show aPDT is an effective and safe method to Psa inactivation on kiwifruit pollen, and MB use is a promising alternative in the control of Psa transmission.

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“…Segundo uma revisão publicada na Antimicrobial Resistance, estima-se que em 2050 a mortalidade por infecções causadas por bactérias resistentes chegue a 10 milhões de casos por ano, superando o número de mortes causadas por câncer e outras doenças responsáveis pela maioria das mortes no mundo. Além disso, o Fórum Econômico Mundial apresentou uma estimativa de que até 2050 haverá uma retração no produto interno bruto mundial de até 3% e uma despesa acumulada de 100 trilhões de dólares, com maior risco de mortalidade para os países com economias emergentes, como nos BRICS (Brasil, Rússia, Índia, China e África do Sul) (PEREIRA et al, 2018). Devido a essa projeção, é necessário o desenvolvimento de novas terapias.…”

Section: Relevância Clínicaunclassified

Erradicação de biofilmes bacterianos mono e multiespécies pela terapia fotodinâmica utilizando a microbiologia preditiva multivariada e novos fotossensibilizadores

Amaral¹

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Agradeço imensamente a minha mãe Marilene Aparecida de Souza pelo apoio, presença (mesmo distante), amor, afeto e disposição de sempre me ajudar. Agradeço por sempre me mostrar que tenho para onde ir e que tenho um lar ao seu lado, independente do lugar.Ao meu namorado Erich Potrich por estar ao meu lado diariamente ao longo de todos os anos em que fiz mestrado e doutorado. Tempo precioso repleto de consolo, crescimento, amizade e alegrias. Agradeço por me ajudar na compreensão de análises multivariadas, pela paciência, companhia diária e força.A minha orientadora Janice Rodrigues Perussi pela excelente orientação durante minha trajetória acadêmica de Mestrado e Doutorado (2014 -2020) na Universidade de São Paulo, pela confiança em meu trabalho, pela dedicação nas correções de meus resumos, artigos e apresentações. Agradeço pelos ensinamentos constantes em várias vertentes da vida. Levarei cada ensinamento com muito apresso e afeto. Agradeço por toda oportunidade a mim concedida.Agradeço a todos os membros do Grupo de Fotossensibilizadores do Instituto de

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Methylene blue internalization and photodynamic action against clinical and ATCC Pseudomonas aeruginosa and Staphyloccocus aureus strains

Cited by 22 publications

References 24 publications

Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains

Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains

Antimicrobial Photodynamic Therapy in the Control of Pseudomonas syringae pv. actinidiae Transmission by Kiwifruit Pollen

Erradicação de biofilmes bacterianos mono e multiespécies pela terapia fotodinâmica utilizando a microbiologia preditiva multivariada e novos fotossensibilizadores

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