Methylene blue dyeing of cellular nuclei during salpingoscopy, a new in- vivo method to evaluate vitality of tubal epithelium

Abstract

The Fallopian tube can be damaged by different noxious substances that may change cellular ultrastructure and function. Alteration of the cell membrane allows the passage of certain aniline dyes, which can stain the nucleus. A total of 310 Fallopian tubes from 163 patients who underwent a surgical or diagnostic laparoscopy during fertility studies was analysed by salpingoscopy. Cellular nuclei were stained by injection of 20 ml of a 10% solution of methylene blue in saline solution (NaCl 10%) through the cervical cannula prior to salpingoscopy. Evaluation of nuclear staining with methylene blue, adhesions, vascular alterations, and the flattening of folds in relation to pregnancy outcome was undertaken. Quantification of salpingoscopic findings was carried out according to a score. Flattening of folds and vascular alterations showed no difference in the pregnant and non-pregnant groups. On the other hand, adhesions and nuclear dyeing were significantly greater in the non-pregnant group (adhesions 13.6 versus 26.8%, P < 0.004, and nuclear dyeing: 25 versus 41.7%, P < 0.009, pregnant versus non-pregnant). Methylene blue dye is a new tool to evaluate in vivo cyto-histological tubal damage, and is a useful and simple method to provide a prognosis of salpingean function.

Fertiloscopie : l'expérience clermontoise

“…Il est admis qu'une dizaine de procédures est suffisante pour cela. Des publications récentes tendent à donner une importance non négligeable à l'étude de la muqueuse tubaire [1,2,12,13,16]. Une salpingoscopie est ainsi communément pratiquée.…”

“…Une pigmentation importante révèle une muqueuse de mauvaise qualité, même si l'épithélium paraît intact. Pour Marconi, le nombre de noyaux cellulaires colorés par le bleu de méthylène et leur intensité va de pair avec une altération de la muqueuse tubaire [13]. Watrelot rapporte un taux d'anomalie de la microsalpingoscopie de 34 % lorsque la fertiloscopie s'avère macroscopiquement normale.…”

Double cuticle barrier in two global pests, the whiteflyTrialeurodes vaporariorumand the bedbugCimex lectularius

“…Bromophenol Blue (Sigma, 0.5% w/v, 0.1% Triton X-100) and Methylene Blue (Sigma, 0.1% w/v, 0.1% Triton X-100) were applied following the same protocol. All three dyes are generally used in histology as unspecific detectors of a variety of molecules (Selman, 1960;Marconi and Quintana, 1998;Fischer et al, 2008). For chloroform washes, insects were incubated in the solvent for 2 min in glass vials prior to staining with dyes at respective temperatures.…”

Fertiloscopie : expérience tunisienne

“…En outre, des publications récentes tendent à donner une importance non négligeable à l'étude de la muqueuse tubaire [1][2][3]6,[19][20][21]. Les données de la salpingoscopie semblent revêtir une importance capitale.…”