Methylation regulation of liver-specific microRNA-122 expression and its effects on the proliferation and apoptosis of hepatocellular carcinoma cells

Xing, Tongjing; Xu, Hong‐Tao; Yu, Wanjun; Jiang, Danfeng

doi:10.4238/2013.september.13.3

Cited by 15 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is not possible to conclusively rule out an association between shift work and liver unhealthiness by our observations of relative young and healthy workers, since adverse effects of circadian disruption on liver health have been found in both animal (Filipski et al, 2009;Konturek et al, 2011) and human studies (Copertaro et al, 2008;Larsson et al, 2009;Ma et al, 2009;Nakamura et al, 1998) and have been supported by studies on gene expression (Szantoova et al, 2011). Determining the exact effects of RSW on liver health in the future may require longer monitoring and other surrogate markers, such as microRNA-122 (Arataki et al, 2013;Spaniel et al, 2013;Waidmann et al, 2012;Xing et al, 2013), aspartate aminotransferase, glutamyl transpeptidase, and bilirubin levels (Bulum et al, 2011). Besides conventional parameters, one can measure the body temperature and monitor the melatonin rhythm, to demonstrate the mismatch between central and peripheral rhythm among shift workers (Cermakian & Boivin, 2009).…”

Section: Discussionmentioning

confidence: 99%

Long-term day-and-night rotating shift work poses a barrier to the normalization of alanine transaminase

Lin

Hsieh

Chen

2013

Chronobiology International

View full text Add to dashboard Cite

To evaluate the impact of day-and-night rotating shift work (RSW) on liver health, we performed a retrospective analysis of the association between long-term RSW exposure and the normalization of plasma alanine transaminase (ALT) levels over a five-year period. The data from physical examinations, blood tests, abdominal sonographic examinations, personal histories, and occupational records were collected from a cohort of workers in a semiconductor manufacturing company. The sample population was divided into three subgroups for analysis, according to self-reported shift work status over the five-year interval: persistent daytime workers, workers exposed intermittently to RSW (i-RSW), and workers exposed persistently to RSW (p-RSW). Records were analyzed for 1196 male workers with an initial mean age of 32.5 years (SD 6.0 years), of whom 821 (68.7%) were identified as rotating shift workers, including 374 i-RSW (31.3%) and 447 p-RSW workers (37.4%). At the beginning of the follow-up, 275 were found to have elevated ALT (e-ALT): 25.1% daytime workers, 23.0% i-RSW workers, and 21.3% p-RSW workers (p = 0.098). Of those with e-ALT at the beginning, 101 workers showed normalized serum ALT levels at the end of five-year follow-up: 40 (10.7%) of 375 daytime workers, 32 (8.6%) of 374 i-RSW workers, and 29 (6.5%) of 447 p-RSW workers (p = 0.016). Compared with the workers having persistent e-ALT at the end of follow-up, the workers normalized serum ALT levels had significantly lesser exposures to RSW during follow-up. By performing multivariate logistic regression analyses, and comparing with the persistent daytime co-workers, after controlling for confounding variables (age, occupational factors, educational levels, lifestyle factors, metabolic syndrome, hepatovirus infection, and fatty liver), analysis indicated that the workers exposed to p-RSW were 46% less likely (OR, 0.54; 95% CI, 0.30-0.95; p = 0.03) to attain normal ALT levels within a five-year interval. These observations demonstrate that persistent day-and-night RSW pose a vigorous obstacle to the normalization of e-ALT among workers with preexisting abnormal liver function. We suggest that workers and managers approach with caution the consideration of assigning or accepting long-term day-and-night RSW when an employee health screening shows evidence of abnormal liver function.

show abstract

Section: Discussionmentioning

confidence: 99%

Long-term day-and-night rotating shift work poses a barrier to the normalization of alanine transaminase

Lin

Hsieh

Chen

2013

Chronobiology International

View full text Add to dashboard Cite

show abstract

“…Its antitumor activity in solid tumors including HCC has been elucidated in previous studies [ 53 ]. Xing et al [ 54 ] have demonstrated that decitabine could facilitate the expression of miR-122 via inhibition of methylation in HCC cells. More importantly, Skårn et al [ 55 ] have demonstrated that miR-142 is epigenetically restrained by DNA methylation.…”

Section: Discussionmentioning

confidence: 99%

A circRNA–miRNA–mRNA network identification for exploring underlying pathogenesis and therapy strategy of hepatocellular carcinoma

et al. 2018

View full text Add to dashboard Cite

BackgroundCircular RNAs (circRNAs) have received increasing attention in human tumor research. However, there are still a large number of unknown circRNAs that need to be deciphered. The aim of this study is to unearth novel circRNAs as well as their action mechanisms in hepatocellular carcinoma (HCC).MethodsA combinative strategy of big data mining, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and computational biology was employed to dig HCC-related circRNAs and to explore their potential action mechanisms. A connectivity map (CMap) analysis was conducted to identify potential therapeutic agents for HCC.ResultsSix differently expressed circRNAs were obtained from three Gene Expression Omnibus microarray datasets (GSE78520, GSE94508 and GSE97332) using the RobustRankAggreg method. Following the RT-qPCR corroboration, three circRNAs (hsa_circRNA_102166, hsa_circRNA_100291 and hsa_circRNA_104515) were selected for further analysis. miRNA response elements of the three circRNAs were predicted. Five circRNA–miRNA interactions including two circRNAs (hsa_circRNA_104515 and hsa_circRNA_100291) and five miRNAs (hsa-miR-1303, hsa-miR-142-5p, hsa-miR-877-5p, hsa-miR-583 and hsa-miR-1276) were identified. Then, 1424 target genes of the above five miRNAs and 3278 differently expressed genes (DEGs) on HCC were collected. By intersecting the miRNA target genes and the DEGs, we acquired 172 overlapped genes. A protein–protein interaction network based on the 172 genes was established, with seven hubgenes (JUN, MYCN, AR, ESR1, FOXO1, IGF1 and CD34) determined from the network. The Gene Oncology, Kyoto Encyclopedia of Genes and Genomes and Reactome enrichment analyses revealed that the seven hubgenes were linked with some cancer-related biological functions and pathways. Additionally, three bioactive chemicals (decitabine, BW-B70C and gefitinib) based on the seven hubgenes were identified as therapeutic options for HCC by the CMap analysis.ConclusionsOur study provides a novel insight into the pathogenesis and therapy of HCC from the circRNA–miRNA–mRNA network view.

show abstract

“…Serum AFP is often elevated in HCC patients, but HCC patients could have normal or low level of AFP, patients with CH and CR could have elevated serum AFP, as shown in our data. Although there are studies in protein or RNA expressions, epigenetics and microRNAs in HCC 15 , fundamentally intensive genomic abnormalities are characteristics of all cancers including HCC, especially at advanced stages. The method we proposed here could identify over two thirds of HCC no more than 50 mm, more than half of HCC no more than 30 mm, with no positive results from 8 CH and CR patients in our study.…”

Section: Discussionmentioning

confidence: 99%

Non-invasive Analysis of Genomic Copy Number Variation in Patients with Hepatocellular Carcinoma by Next Generation DNA Sequencing

Zhu²,

et al. 2015

J. Cancer

View full text Add to dashboard Cite

To explore new molecular diagnosis approaches for early detection and differential diagnosis of hepatocellular carcinoma (HCC), we analyzed genomic copy number variations (CNV) using plasma cell-free DNA from patients with HCC by next generation DNA sequencing. Plasma samples from 31 patients with HCC and 8 patients with chronic hepatitis or cirrhosis were analyzed. In HCC group, most samples with large tumor size (tumor dimension greater than 50 mm) showed CNVs that are visually recognizable at chromosome CNV plots, few samples with small tumor and none samples with chronic liver diseases showed CNVs recognizable at CNV plots. CNV Z score analysis showed significant CNVs in samples with HCC and chronic liver diseases although more significant changes were found in HCC group, some are differentially valuable (such as gain in 1q, 7q, and 19q in HCC), while others are less differentially valuable (such as loss in 4q, 13q, gain in 17q, 22q). We proposed a CNV scoring method that generated positive result in 26 of the 31 HCC patients (83.9%) or 11 of the 16 HCC with tumor dimension 50 mm or less (68.8%) or 4 of the 7 HCC with tumor dimension 30 mm or less (57.1%), while all the 8 samples with chronic hepatitis or cirrhosis scored negative. Ten HCC patients had normal or low serum AFP levels, among them, 7 were scored positive by CNV analysis, including 4 with tumor dimension 50 mm or less. Our study suggested that non-invasive genomic CNV analysis using plasma samples could be a valuable tool for early detection and differential diagnosis of HCC. Although CNV analysis itself cannot establish the diagnosis, it can help identify patients at high risk for HCC among patients with chronic liver diseases, which would prompt closer and more frequent surveillance for early tumor detection and intervention.

show abstract

Methylation regulation of liver-specific microRNA-122 expression and its effects on the proliferation and apoptosis of hepatocellular carcinoma cells

Cited by 15 publications

References 17 publications

Long-term day-and-night rotating shift work poses a barrier to the normalization of alanine transaminase

Long-term day-and-night rotating shift work poses a barrier to the normalization of alanine transaminase

A circRNA–miRNA–mRNA network identification for exploring underlying pathogenesis and therapy strategy of hepatocellular carcinoma

Non-invasive Analysis of Genomic Copy Number Variation in Patients with Hepatocellular Carcinoma by Next Generation DNA Sequencing

Contact Info

Product

Resources

About