Methylation of the C19MC microRNA locus in the placenta: association with maternal and chilhood body size

Prats‐Puig, Anna; Xargay-Torrent, Sílvia; Carreras‐Badosa, Gemma; Mas-Parés, Berta; Bassols, Judit; Petry, Clive J.; Girardot, Michael; Zegher, Francis de; Ibáñez, Lourdes; Feil, Robert; López‐Bermejo, Abel

doi:10.1038/s41366-019-0450-9

Cited by 11 publications

(8 citation statements)

References 52 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, the expression level of miR520a-3p and miR-520a-3p in fetal cord blood had positive correlations with newborn body weight. These results are also supported by a very recent study that lower levels of the placenta C19MC methylation status was associated with increased maternal pre-pregnancy weight, and with higher weight and fat mass of their offspring [41]. Therefore, it is plausible that declines in C19MC methylation status in the placenta of overweight/obese pregnant women upregulates the expression of C19MC miRNA, which adversely affects the gene expression pattern and phenotype of the fetus.…”

Section: Discussionsupporting

confidence: 71%

Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood

Jia¹,

Wang²,

Quan³

et al. 2020

Nutr Metab (Lond)

View full text Add to dashboard Cite

Background: To determine if overweight/obese pregnant women have altered microRNA expression patterns in fetal umbilical cord blood that may affect the development of offspring. Methods: Umbilical cord blood samples were obtained from the fetuses of 34 overweight/obese and 32 normalweight women after delivery. Next generation sequencing (NGS) analyzed their miRNA expression patterns. miRanda and TargetScan databases were used to predict the miRNAs' target genes followed by analyses of Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to perform function grouping and pathway analyses. qRT-PCR verified the identity of differentially expressed miRNAs that were revealed in the NGS results. Results: There was a positive correlation between newborn body weight and pregestational BMI of pregnant individuals (r = 0.48, P < 0.001). One hundred and eight miRNAs were differentially expressed between the normal and overweight/obese groups, which target genes were enriched in the metabolic pathway. Five C19MC miRNAs (miR-516a-5p, miR-516b-5p, miR-520a-3p, miR-1323, miR-523-5p) were upregulated in the overweight/obese group. Target enrichment analysis suggests their involvement in post-embryonic development, lipid and glucose homeostasis, T cell differentiation and nervous system development. Conclusions: C19MC miRNA expression upregulation in the fetal circulation during the gestation of overweight/ obese pregnant women may contribute to altered multisystem metabolic pathway development in their offspring.

show abstract

Section: Discussionsupporting

confidence: 71%

Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood

Jia¹,

Wang²,

Quan³

et al. 2020

Nutr Metab (Lond)

View full text Add to dashboard Cite

show abstract

“…Previously reported imprinted expression of the miRNA cluster located on chromosome 19 (C19MC) [31,32] was also evident in our data. Previous work further linked the amplitude of the imprinting effect with the mother's BMI [48]. Our analysis of demographic variables indicated that the relationship between C19MC cluster imprinting and mother's BMI depends on the mother's weight but not the height.…”

Section: Discussionmentioning

confidence: 55%

Variation of MicroRNA Expression in the Human Placenta Driven by Population Identity and Sex of the Newborn

Guo

Huang

Jiang

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundAnalysis of lymphocyte cell lines revealed substantial differences in the expression of mRNA and microRNA (miRNA) among human populations. The extent of such population-associated differences in actual human tissues remains largely unexplored. The placenta is one of the few solid human tissues that can be collected in substantial numbers in a controlled manner, enabling quantitative analysis of transient biomolecules such as RNA transcripts. Here, we analyzed microRNA (miRNA) expression in human placental samples derived from 36 individuals representing four genetically distinct human populations: African Americans, European-Americans, South Asians, and East Asians. All samples were collected at the same hospital following a unified protocol, thus minimizing potential biases that might influence the results.ResultsSequence analysis of the miRNA fraction yielded 938 annotated and 70 novel miRNA transcripts expressed in the placenta. Of them, 82 (9%) of annotated and 11 (16%) of novel miRNAs displayed quantitative expression differences among populations, generally reflecting reported genetic and mRNA-expression-based distances. Several co-expressed miRNA clusters stood out from the rest of the population-associated differences in terms of miRNA evolutionary age, tissue-specificity, and disease-association characteristics. Among three non-environmental influenced demographic parameters, the second largest contributor to miRNA expression variation after population was the sex of the newborn, with 32 miRNAs (3% of detected) exhibiting significant expression differences depending on whether the newborn was male or female. Male-associated miRNAs were evolutionarily younger and correlated inversely with the expression of target mRNA involved in neuron-related functions. In contrast, both male and female-associated miRNAs appeared to mediate different types of hormonal responses. Demographic factors further affected reported imprinted expression of 66 placental miRNAs: the imprinting strength correlated with the mother’s weight, but not height. ConclusionsOur results showed that among 12 assessed demographic variables, population affiliation and fetal sex had a substantial influence on miRNA expression variation among human placental samples. The effect of newborn-sex-associated miRNA differences further led to expression inhibition of the target genes clustering in specific functional pathways. By contrast, population-driven miRNA differences might mainly represent neutral changes with minimal functional impacts.

show abstract

“…Previously reported imprinted expression of the miRNA cluster located on chromosome 19 (C19MC) [ 31 , 32 ] was also evident in our data. Previous work further linked the amplitude of the imprinting effect with the mother’s BMI [ 48 ]. Our analysis of demographic variables indicated that the relationship between C19MC cluster imprinting and mother’s BMI depends on the mother’s weight but not the height.…”

Section: Discussionmentioning

confidence: 99%

Variation of microRNA expression in the human placenta driven by population identity and sex of the newborn

et al. 2021

View full text Add to dashboard Cite

Background Analysis of lymphocyte cell lines revealed substantial differences in the expression of mRNA and microRNA (miRNA) among human populations. The extent of such population-associated differences in actual human tissues remains largely unexplored. The placenta is one of the few solid human tissues that can be collected in substantial numbers in a controlled manner, enabling quantitative analysis of transient biomolecules such as RNA transcripts. Here, we analyzed microRNA (miRNA) expression in human placental samples derived from 36 individuals representing four genetically distinct human populations: African Americans, European Americans, South Asians, and East Asians. All samples were collected at the same hospital following a unified protocol, thus minimizing potential biases that might influence the results. Results Sequence analysis of the miRNA fraction yielded 938 annotated and 70 novel miRNA transcripts expressed in the placenta. Of them, 82 (9%) of annotated and 11 (16%) of novel miRNAs displayed quantitative expression differences among populations, generally reflecting reported genetic and mRNA-expression-based distances. Several co-expressed miRNA clusters stood out from the rest of the population-associated differences in terms of miRNA evolutionary age, tissue-specificity, and disease-association characteristics. Among three non-environmental influenced demographic parameters, the second largest contributor to miRNA expression variation after population was the sex of the newborn, with 32 miRNAs (3% of detected) exhibiting significant expression differences depending on whether the newborn was male or female. Male-associated miRNAs were evolutionarily younger and correlated inversely with the expression of target mRNA involved in neuron-related functions. In contrast, both male and female-associated miRNAs appeared to mediate different types of hormonal responses. Demographic factors further affected reported imprinted expression of 66 placental miRNAs: the imprinting strength correlated with the mother’s weight, but not height. Conclusions Our results showed that among 12 assessed demographic variables, population affiliation and fetal sex had a substantial influence on miRNA expression variation among human placental samples. The effect of newborn-sex-associated miRNA differences further led to expression inhibition of the target genes clustering in specific functional pathways. By contrast, population-driven miRNA differences might mainly represent neutral changes with minimal functional impacts.

show abstract

Methylation of the C19MC microRNA locus in the placenta: association with maternal and chilhood body size

Cited by 11 publications

References 52 publications

Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood

Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood

Variation of MicroRNA Expression in the Human Placenta Driven by Population Identity and Sex of the Newborn

Variation of microRNA expression in the human placenta driven by population identity and sex of the newborn

Contact Info

Product

Resources

About