Methylation of <i>AcGST1</i> Is Associated with Anthocyanin Accumulation in the Kiwifruit Outer Pericarp

Zhang, Xuefeng; Zhang, Kun; Guo, Yuqi; Lv, Xiaoyu; Wu, Meijing; Deng, Honghong; Xie, Yue; Li, Mingzhang; Wang, Jin; Lin, Lijin; Lv, Xiulan; Xia, Hui; Liang, Dong

doi:10.1021/acs.jafc.3c03029

Cited by 2 publications

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“…The following supporting information can be downloaded at https:// www.mdpi.com/article/10.3390/plants13101316/s1, Table S1: Analysis of protein physicochemical properties and prediction of subcellular localization of VcGSTs; Table S2: Fifteen conserved motifs of VcGST proteins; Table S3: Interspecies collinearity analysis; Table S4: Pearson correlation coefficients between relative expression changes of 12 genes and anthocyanin accumulation; Table S5: Gene primers used for real-time fluorescence quantification; Table S6: The 17 identified anthocyanin intracellular transport GST proteins used for the analysis of potential anthocyanin transport GST proteins in blueberries. References [71][72][73][74][75][76][77][78][79][80][81][82] are cited in the supplementary materials.…”

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confidence: 99%

Identification and Characterization of the Glutathione S-Transferase Gene Family in Blueberry (Vaccinium corymbosum) and Their Potential Roles in Anthocyanin Intracellular Transportation

Wang,

Dong,

et al. 2024

Plants

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The glutathione S-transferases (GSTs, EC 2.5.1.18) constitute a versatile enzyme family with pivotal roles in plant stress responses and detoxification processes. Recent discoveries attributed the additional function of facilitating anthocyanin intracellular transportation in plants to GSTs. Our study identified 178 VcGST genes from 12 distinct subfamilies in the blueberry genome. An uneven distribution was observed among these genes across blueberry’s chromosomes. Members within the same subfamily displayed homogeneity in gene structure and conserved protein motifs, whereas marked divergence was noted among subfamilies. Functional annotations revealed that VcGSTs were significantly enriched in several gene ontology and KEGG pathway categories. Promoter regions of VcGST genes predominantly contain light-responsive, MYB-binding, and stress-responsive elements. The majority of VcGST genes are subject to purifying selection, with whole-genome duplication or segmental duplication serving as key processes that drive the expansion of the VcGST gene family. Notably, during the ripening of the blueberry fruit, 100 VcGST genes were highly expressed, and the expression patterns of 24 of these genes demonstrated a strong correlation with the dynamic content of fruit anthocyanins. Further analysis identified VcGSTF8, VcGSTF20, and VcGSTF22 as prime candidates of VcGST genes involved in the anthocyanin intracellular transport. This study provides a reference for the exploration of anthocyanin intracellular transport mechanisms and paves the way for investigating the spectrum of GST functions in blueberries.

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