Methylation changes in muscle and liver tissues of male and female mice exposed to acute and chronic low-dose X-ray-irradiation

Kovalchuk, Olga; Burke, Paula; Besplug, Jill; Slovack, Mark; Filkowski, Jody; Pogribny, Igor P.

doi:10.1016/j.mrfmmm.2003.12.016

Cited by 116 publications

(76 citation statements)

References 33 publications

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“…On the contrary, they concluded that β-carotene, vitamin A, and vitamin E seem to increase the risk of death. Concerns not only include rescuing cells that still sustain DNA mutations, but also the altered methylation patterns that can result in genomic instability 97 . An approach to target damaged cells for apoptosis may be advantageous for chronic exposures to galactic cosmic radiation (GCR).…”

Section: Radioprotective Agentsmentioning

confidence: 99%

Heavy ion carcinogenesis and human space exploration

Durante¹,

Cucinotta²

2008

Nat Rev Cancer

493

324

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Prior to the human exploration of Mars or long duration stays on the Earth's moon, the risk of cancer and other diseases from space radiation must be accurately estimated and mitigated. Space radiation, comprised of energetic protons and heavy nuclei, has been show to produce distinct biological damage compared to radiation on Earth, leading to large uncertainties in the projection of cancer and other health risks, while obscuring evaluation of the effectiveness of possible countermeasures. Here, we describe how research in cancer radiobiology can support human missions to Mars and other planets. Introduction:

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Section: Radioprotective Agentsmentioning

confidence: 99%

Heavy ion carcinogenesis and human space exploration

Durante¹,

Cucinotta²

2008

Nat Rev Cancer

493

324

View full text Add to dashboard Cite

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“…For example, it was revealed that there are numerous epigenetic differences in MZ twins and that such epigenetic variation can serve as a new source of putative molecular substrates for discordance of MZ twins [19]. A number of environmental factors, both chemical and radiation have been found to alter the epigenome, including DNA methylation [5][6][7][8][9][10]. It should be emphasized that most of the investigations devoted to evaluation of the epigenetic effects of radiation (X -rays and γ -rays, range of doses of 0.1 to 2.5 Gy) was performed using cell cloning techniques.…”

Section: Discussionmentioning

confidence: 99%

“…Radiation also did not affect significantly methylation status of MGMT promoter. It was proved that chronic low-dose radiation exposure is a more potent inducer of epigenetic effects than the acute exposure [9].…”

Section: Discussionmentioning

confidence: 99%

“…Сhanges in DNA methylation patterns include global hypomethylation, genespecific hypermethylation/ hypomethylation and loss of imprinting. The reality of these epigenetic modifications, induced by radiation, is shown in experiments using cell cultures and different animal models [5][6][7][8][9]. The bulk of the research is devoted to the assessment of DNA methylation in long interspersed nuclear element-1 (LINE-1) and Alu repetitive elements as a surrogate of genome-wide methylation.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, hypermethylation CGIs of genes involved in carcinogenesis (tumor--suppressor genes and a number of other genes) leads to transcriptional repression and is revealed in cells of different malignant tumors [12][13][14][15]. At the same time, chronic X-ray irradiation (50 cGy, 10 days) of mice also induced hypermethylation of the p16 tumor-supressor gene in the cells of the liver in a sex-specific manner [9]. However, there are no reports on the hypermethylation effects of radiation exposure in humans.…”

Section: Introductionmentioning

confidence: 99%

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The study of hypermethylation in blood leukocytes of irradiated parents and their children

et al. 2014

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Background: Accumulation of evidence about the epigenetic regulation of genome function suggests the necessity to explore new aspects of the genotoxic action of radiation on the human body. Methodology: A methylation-sensitive PCR assay was used to analyze promoter methylation of p16/CDKN2A, p14/ARF, RASSF1A and GSTP1 genes in blood leukocytes from 103 unirradiated volunteers and 104 irradiated subjects (83 Chernobyl Nuclear Power Plant liquidators and 21 nuclear specialists). Additionally, 21 families whose fathers were nuclear specialists were examined. Results: A significantly elevated frequency of individuals with abnormal methylation of p16/CDKN2A and GSTP1 genes was revealed in the exposed group compared to the control group (p = 0.0097 and p = 0.005, respectively). The occurrence of promoter methylation of RASSF1A gene significantly correlated with aging both in the control group (r = 0, 213; p = 0.006) and in the exposed individuals (r = 0, 212; p = 0,031). No methylated genes were found in the offspring of control families. Conclusion: Our study showed for the fist time that prolonged radiation exposure at low and medium doses is associated with hypermethylation of genes involved in the basic protective functions of cells; an effect that is persistent in blood leukocytes for significant periods after irradiation.

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Analysis of global DNA methylation changes in human keratinocytes immediately following exposure to a 900 MHz radiofrequency field

Cantu

Butterworth

Peralta

et al. 2023

Bioelectromagnetics

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The increasing use of nonionizing radiofrequency electromagnetic fields (RF-EMFs) in a wide range of technologies necessitates studies to further understanding of biological effects from exposures to such forms of electromagnetic fields. While previous studies have described mechanisms for cellular changes occurring following exposure to low-intensity RF-EMFs, the role of molecular epigenetics has not been thoroughly investigated. Specifically unresolved is the effect of RF-EMFs on deoxyribonucleic acid (DNA) methylation, which is a powerful epigenetic process, used by cells to regulate gene expression. DNA methylation is dynamic and can be rapidly triggered in response to external stimuli such as exposure to RF-EMFs. In the present study, we performed a global analysis of DNA methylation patterns in human keratinocytes exposed to 900 MHz RF-EMFs for 1 h at a low dose rate (estimated mean specific absorption rate (SAR) < 10 mW/kg). We used a custom system to allow stable exposure of cell cultures to RF-EMFs under biologically relevant conditions (37 °C, 5% CO 2 , 95% humidity). We performed whole genome bisulfite sequencing directly following RF-EMF exposure to examine the immediate changes in DNA methylation patterns and identify early differentially methylated genes in RF-EMF-exposed keratinocytes. By correlating global gene expression to whole genome bisulfite sequencing, we identified six common targets that were both differentially methylated and differentially expressed in response to RF-EMF exposure. The results highlight a potential epigenetic role in the cellular response to RF-EMFs. Particularly, the six identified targets may potentially be developed as epigenetic biomarkers for immediate responses to RF-EMF exposure. Bioelectromagnetics. 44:77-89, 2023.

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Methylation changes in muscle and liver tissues of male and female mice exposed to acute and chronic low-dose X-ray-irradiation

Cited by 116 publications

References 33 publications

Heavy ion carcinogenesis and human space exploration

Heavy ion carcinogenesis and human space exploration

The study of hypermethylation in blood leukocytes of irradiated parents and their children

Analysis of global DNA methylation changes in human keratinocytes immediately following exposure to a 900 MHz radiofrequency field

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