Methylation and Expression of FTO and PLAG1 Genes in Childhood Obesity: Insight into Anthropometric Parameters and Glucose–Lipid Metabolism

Czogała, Wojciech; Czogała, Małgorzata; Strojny, Wojciech; Wątor, Gracjan; Wołkow, Paweł; Wójcik, Małgorzata; Bik-Multanowski, Mirosław; Tomasik, Przemysław; Wędrychowicz, Andrzej; Kowalczyk, Wojciech; Miklusiak, Karol; Łazarczyk, Agnieszka; Hałubiec, Przemysław; Skoczeń, Szymon

doi:10.3390/nu13051683

Cited by 16 publications

(16 citation statements)

References 83 publications

(98 reference statements)

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“…In our previous research, we demonstrated differences in the methylation and expression of the FTO gene between healthy and obese children [55]. In the current study, we showed that the expression and methylation level of the FTO gene and the expression of the PLAG1 gene are related to the concentrations of selected adipokines and gastrointestinal tract hormones.…”

Section: Discussionsupporting

confidence: 56%

“…However, the expression and methylation of the FTO gene have not been described yet. In our previous study, we found that the obese children showed significantly higher expression of the FTO gene in comparison to the healthy controls, as well as significantly higher methylation values for the FTO gene [55], which confirmed a considerable role of the FTO gene in the development of obesity.…”

Section: Fto Genementioning

confidence: 56%

“…According to the GWAS Catalog [86,87], PLAG1 polymorphisms are associated with traits such as birth weight [52], lean body mass [53] and BMI-adjusted hip circumference [54]. In our previous research, we showed that PLAG1 expression correlated positively with body fat percent in children [55]. The possible involvement of PLAG1 in fat tissue growth suggests that it could also influence the amount of synthesized adipokines.…”

Section: Plag1 Genementioning

confidence: 99%

“…Out of the two studied genes, the analysis included the methylation data only for the FTO gene. The PLAG1 methylation was not included in the calculations due to the fact that the CpG sites within this gene, which we initially intended to investigate, proved to be of no influence on the expression of the gene [55].…”

Section: Limitationsmentioning

confidence: 99%

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FTO and PLAG1 Genes Expression and FTO Methylation Predict Changes in Circulating Levels of Adipokines and Gastrointestinal Peptides in Children

et al. 2021

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Adipokines and gastrointestinal tract hormones are important metabolic parameters, and both epigenetic factors and differential gene expression patterns may be associated with the alterations in their concentrations in children. The function of the FTO gene (FTO alpha-ketoglutarate dependent dioxygenase) in the regulation of the global metabolic rate is well described, whereas the influence of protooncogene PLAG1 (PLAG1 zinc finger) is still not fully understood. A cross-sectional study on a group of 26 children with various BMI values (15.3–41.7; median 28) was carried out. The aim was to evaluate the dependencies between the level of methylation and expression of aforementioned genes with the concentration of selected gastrointestinal tract hormones and adipokines in children. Expression and methylation were measured in peripheral blood mononuclear DNA by a microarray technique and a restriction enzyme method, respectively. All peptide concentrations were determined using the enzyme immunoassay method. The expression level of both FTO and PLAG1 genes was statistically significantly related to the concentration of adipokines: negatively for apelin and leptin receptor, and positively for leptin. Furthermore, both FTO methylation and expression negatively correlated with the concentration of resistin and visfatin. Cholecystokinin was negatively correlated, whereas fibroblast growth factor 21 positively correlated with methylation and expression of the FTO gene, while FTO and PLAG1 expression was negatively associated with the level of cholecystokinin and glucagon-like peptide-1. The PLAG1 gene expression predicts an increase in leptin and decrease in ghrelin levels. Our results indicate that the FTO gene correlates with the concentration of hormones produced by the adipose tissue and gastrointestinal tract, and PLAG1 gene may be involved in adiposity pathogenesis. However, the exact molecular mechanisms still need to be clarified.

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Section: Discussionsupporting

confidence: 56%

Section: Fto Genementioning

confidence: 56%

Section: Plag1 Genementioning

confidence: 99%

Section: Limitationsmentioning

confidence: 99%

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FTO and PLAG1 Genes Expression and FTO Methylation Predict Changes in Circulating Levels of Adipokines and Gastrointestinal Peptides in Children

et al. 2021

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“…Both hereditary and epigenetic factors influence childhood obesity. In a cross-sectional study conducted by Czogała et al, six obese (aged 6.6-17.7 years) and 10 healthy (aged 11.4-16.9 years) children were followed to analyze the expression of the FTO gene (FTO alpha-ketoglutarate dependent dioxygenase), and protooncogene PLAG1 (PLAG1 zinc finger) in the development of childhood obesity [ 23 ]. Because these genes regulate fat tissue cell metabolism and are strongly related to body weight, the goal of this study was to examine the genome expression and methylation of the FTO and PLAG1 genes in overweight and obese children.…”

Section: Reviewmentioning

confidence: 99%

A Systematic Review of the Long-Term Trajectory of Hemodynamics and Body Composition in Childhood Obesity

Suri¹,

Suri²,

Kumar³

et al. 2021

Cureus

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Obesity has long been cited as a significant risk factor for high blood pressure, with long-term exposure linked with an increased incidence of carotid artery atherosclerosis. However, as obesity is becoming more common in young-aged children, it is important to recognize combinational factors like lifestyle, socioeconomic, and genetic factors as a cause. Increasing weight during childhood, on the other hand, is a clinically significant challenge for many physicians. It is critical to identify these risk factors since early prevention (primordial prevention) or treatment (primary prevention) to reverse the potential risks is much more likely to be effective. The objective of this review was to explore the relationship between lifestyle, anthropometric, and genetic factors and cardiometabolic risk factors. We are hopeful that our findings may aid in the primary prevention of hypertension in children and the prevention of any related cardiovascular complications that may arise later in life as a result of obesity. The synthesis of this systematic review includes a total of 15 studies using defined criteria, published up to October 2021. In addition, a total of 2,397 articles were found through an initial electronic database search and included a total of 38,182 participants. Studies explored included one or more of the following cardiovascular risk factors: body mass index (BMI), systolic blood pressure (SBP), diastolic blood pressure (DBP), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and triglycerides (TG). The findings of this study support the notion that childhood obesity may be a risk factor for a variety of adult cardiovascular disease risk factors. Several behavioral, genetic, and anthropometric factors are linked to the development of obesity during early ages, overall creating challenges in cardiovascular health during adulthood. As a result, addressing the risk factors for childhood hypertension would be advantageous for the primary prevention of its sequelae in adulthood.

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Frizzled receptors and SFRP5 in lipid metabolism: Current findings and potential applications

Chu

Nguyen

2023

Progress in Molecular Biology and Translational Science

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Methylation and Expression of FTO and PLAG1 Genes in Childhood Obesity: Insight into Anthropometric Parameters and Glucose–Lipid Metabolism

Cited by 16 publications

References 83 publications

FTO and PLAG1 Genes Expression and FTO Methylation Predict Changes in Circulating Levels of Adipokines and Gastrointestinal Peptides in Children

FTO and PLAG1 Genes Expression and FTO Methylation Predict Changes in Circulating Levels of Adipokines and Gastrointestinal Peptides in Children

A Systematic Review of the Long-Term Trajectory of Hemodynamics and Body Composition in Childhood Obesity

Frizzled receptors and SFRP5 in lipid metabolism: Current findings and potential applications

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